Cereal Research Communications

, Volume 38, Issue 1, pp 1–14 | Cite as

Characterization and classification of γ-gliadin multigene sequences from Aegilops section Sitopsis

  • Z. Huang
  • H. Long
  • Y. M. WeiEmail author
  • P. F. Qi
  • Z. H. Yan
  • Y. L. ZhengEmail author
Open Access


The most abundant seed storage proteins of wheat are gliadins and glutenins. Gliadins, including α/β, γ and ω types, are normally monomeric proteins and account for about 50% of the gluten proteins. In this study, 55 sequences of γ-gliadin genes were obtained from species of Sitopsis section, the deduced B genome donors of wheat. Despite the high sequence similarities to the known γ-gliadin genes, extensive variations were also found. Using the extensive sequence information deposited in database and obtained in this study, a comprehensive classification of the γ-gliadin multigene families were performed based on the primary structures and phylogenic analysis. All the γ-gliadin genes analyzed could be divided into 2 types, which contain 8 and 9 cysteines, respectively. Type I (with 8 cysteines) and type II (with 9 cysteines) are further classified to 7 and 4 groups, respectively, and several subgroups are also identified. The genes derived from A, B and D genomes of common wheat were clustered distinctly, indicating that there was apparent genomic specificity in γ-gliadins genes. Besides the high homology between γ-gliadin genes from Sitopsis species and B genome of wheat, some unique groups or subgroups were also identified in Sitopsis section, suggesting that it could be considered as a valuable source of γ-gliadin genes. The comparison of deduced primary structures of each group and/or subgroup was conducted, from which their evolution and quality properties were also speculated.


wheat Aegilops seed storage protein γ-gliadin 


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© Akadémiai Kiadó, Budapest 2010

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Authors and Affiliations

  1. 1.Dujiangyan Campus, Sichuan Agricultural UniversityDujiangyan, SichuanChina
  2. 2.Chengdu Institute of Biology ChengduChinese Academy of SciencesSichuanChina
  3. 3.Triticeae Research InstituteSichuan Agricultural UniversityYaan, SichuanChina
  4. 4.Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of AgricultureThe People’s Republic of ChinaYaan, SichuanChina

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