Chlorophyll content is positively correlated with photosynthetic rate. However, little is known about the genetic correlation between grain yield and chlorophyll content in the same wheat mapping population. The primary goal of the study was to detect the genetic basis of grain yield and chlorophyll content and their possible roles in the genetic improvement of grain yield in wheat. Here, quantitative trait loci (QTLs) for grain yield and chlorophyll content were studied using a set of 168 doubled haploid (DH) lines derived from a cross between two elite Chinese wheat cultivars, Huapei 3×Yumai 57. The DH population and parents were evaluated for grain yield and chlorophyll content in three environments. A total of 11 additive QTLs and 6 pairs of epistatic QTLs were detected for grain yield and chlorophyll content. Loci, such as Xcfd53, Xwmc718, and Xwmc215 on chromosomes (e.g. 2D, 4A, and 5D) simultaneously controling grain yield and chlorophyll content, showed tight linkages or pleiotropisms. Three novel major QTLs, qGY5D, qChla5D, and qChlb5D, closely linked with the PCR marker Xwmc215 on chromosome 5D, accounted for 10.32%, 12.95%, and 23.29% of the phenotypic variance, respectively. The favorable alleles came from Yumai 57.
Börner, A., Schumann, E., Furste, A., Coster, H., Leithold, B., Röder, M.S., Weber, W.E. 2002. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor. Appl. Genet. 105:921–936.
Cao, W.D., Jia, J.Z., Jin, J.Y. 2004. Identification and interaction analysis of QTL for chlorophyll content in wheat seedlings. Plant Nutr. Ferti. Sci. 10:473–478.
Churchill, G.A., Doerge, R.W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138:963–971.
Fang, P., Yu, X.M., Zhu, R.Q., Wu, P. 2004. QTLs for rice leaf chlorophyll content under low N stress. Pedosphere 14:145–150.
Groos, C., Robert, N., Bervas, E., Charmet, G. 2003. Genetic analysis of grain protein content, grain yield and thousand-kernel weight in bread wheat. Theor. Appl. Genet. 106:1032–1040.
Guo, C.Q., Bai, Z.A., Liao, P.A., Jin, W.K. 2004. New high quality and yield wheat variety Yumai 57. China Seed Industry, 4:54.
Hai, Y., Kang, M.H. 2007. Breeding of Huapei 3 new wheat variety with high yield and early maturing. Henan Agric. Sci. 5:36–37.
Hanocq, E., Laperche, A., Jaminon, O., Lainé, A.L., LeGouis, J. 2007. Most significant genome regions involved in the control of earliness traits in bread wheat, as revealed by QTL meta-analysis. Theor. Appl. Genet. 114:569–584
Huang, X.Q., Kempf, H., Ganal, M.W., Röder, M.S. 2004. Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:933–943.
Huang, X.Q., Chloutier, S., LyCad, L., Radovanovic, N., Humphreys, D.G., Noll, J.S., Somers, D.J., Brown, P.D. 2006. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor. Appl. Genet. 113:753–766.
Knapp, S.J., Stroup, W.W., Ross, W.M. 1985. Exact convidence intervals for heritability on a progeny mean basis. Crop Sci. 25:192–194.
Kuchel, H., Williams, K., Langridge, P., Eagles, H.A., Jefferies, S.P. 2007. Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theor. Appl. Genet. 115:1015–1027.
Li, S.S., Jia, J.Z., Wei, X.Y., Zhang, X.C., Li, L.Z., Chen, H.M., Fan, Y.D., Sun, H.Y., Zhao, X.H., Lei, T.D., Xu, Y.F., Jiang, F.S., Wang, H.G., Li, L.H. 2007. An intervarietal genetic map and QTL analysis for yield traits in wheat. Mol. Breeding 20:167–178.
Marza, F., Bai, G.H., Carver, B.F., Zhou, W.C. 2006. Quantitative trait loci for yield and related traits in the wheat population Nin7840 × Clark. Theor. Appl. Genet. 112:688–698.
McCartney, C.A., Somers, D.J., Humphreys, D.G., Lukow, O., Ames, N., Noll, J., Cloutier, S., McCallum, B.D. 2005. Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross ‘RL4452’ × ‘AC Domain’. Genome 48:870–88
Motzo, R., Giunta, F. 2007. The effect of breeding on the phenology of Italian durum wheats: From landraces to modern cultivars. Eur. J. Agron. 26:462–470.
Narasimhamoorthy, B., Gill, B.S., Fritz, A.K., Nelson, J.C., Brown-Guedira, G.L. 2006. Advanced backcross QTL analysis of a hard winter wheat × synthetic wheat population. Theor. Appl. Genet. 112:787–796.
Porra, R.J., Thompson, W.A., Kriedemann, P.E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta 975:384–394.
Quarrie, S.A., Pekic Quarrie, S., Radosevic, R., Rancic, D., Kaminska, A., Barnes, J.D., Leverington, M., Ceoloni, C., Dodig, D. 2006. Dissecting a wheat QTL for yield present in a range of environments: from the QTL to candidate genes. J. Experimental Botany 57: 2627–2637.
Rebetzke, G.J., Ellis, M.H., Bonnett, D.G., Richards, R.A. 2007. Molecular mapping of genes for coleoptile growth in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 114:1173–1183.
Rebetzke, G.J., Richards, R.A., Fischer, V.M., Mickelson, B.J. 1999. Breeding long coleoptile, reduced height wheats. Euphytica 106:159–168.
Shen, B., Zhuang, J.Y., Zhang, K.Q., Dai, W.M., Lu, Y., Fu, L.Q., Ding, J.M., Zheng, K.L. 2007. QTL mapping of chlorophyll content in rice. Agric. Sci. Sinic. 6:17–24.
Thomas, H., Smart, C.M. 1993. Crops that stay green. Annals Appl. Biol. 123:193–203.
Thomas, J.A., Jeffrey, A.C., Atsuko, K., David, M.K. 2005. Regulating the proton budget of higher plant photosynthesis. Proc. Natl. Acad. Sci. USA 102:9709–9713.
Wang, B., Lan, T., Wu, W.R., Li, W.M. 2003. Mapping of QTLs controlling chlorophyll content in rice (Oryza sativa L.). Acta Genet. Sin. 30:1127–1132.
Wang, D.L., Zhu, J., Li, Z.K., Paterson, A.H. 1999. Mapping QTLs with epistatic effects and QTL× environment interactions by mixed linear model approaches. Theor. Appl. Genet. 99:1255–1264.
Wang, F.H., Wang, G.X., Lia, X.Y., Huang, J.L., Zheng, J.K. 2008. Heredity, physiology and mapping of a chlorophyll content gene of rice (Oryza sativa L.). J. Plant Physiol. 165:324–330.
Yang, D.L., Jing, R.L., Chang, X.P., Li, W. 2007. Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat (Triticum aestivum). J. Integ. Plant Biol. 49:646–654.
Yang, G., Li, S., Feng, L., Kong, J., Li, H., Li, Y. 2006. Analysis of QTL underlying the traits relative to the chlorophyll content of the flag leaf in rice. J. Wuhan Univ. 52:751–756.
Yang, J., Zhu, J. 2005. Methods for predicting superior genotypes under multiple environments based on QTL effects. Theor. Appl. Genet. 110:1268–1274.
Zhang, K.P., Tian, J.C., Zhao, L., Liu, B., Chen, G.F. 2009. Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars. Genetica 135:257–265.
Zhang, Z.L. 1990. Guide to plant physiology experiments. Higher Education Press, Beijing.
Communicated by D. Habash
About this article
Cite this article
Zhang, K., Zhang, Y., Chen, G. et al. Genetic analysis of grain yield and leaf chlorophyll content in common wheat. CEREAL RESEARCH COMMUNICATIONS 37, 499–511 (2009). https://doi.org/10.1556/CRC.37.2009.4.3
- chlorophyll content
- grain yield
- marker-assisted selection
- quantitative trait loci
- Triticum aestivum L.