Genetic analysis of grain yield and leaf chlorophyll content in common wheat
Chlorophyll content is positively correlated with photosynthetic rate. However, little is known about the genetic correlation between grain yield and chlorophyll content in the same wheat mapping population. The primary goal of the study was to detect the genetic basis of grain yield and chlorophyll content and their possible roles in the genetic improvement of grain yield in wheat. Here, quantitative trait loci (QTLs) for grain yield and chlorophyll content were studied using a set of 168 doubled haploid (DH) lines derived from a cross between two elite Chinese wheat cultivars, Huapei 3×Yumai 57. The DH population and parents were evaluated for grain yield and chlorophyll content in three environments. A total of 11 additive QTLs and 6 pairs of epistatic QTLs were detected for grain yield and chlorophyll content. Loci, such as Xcfd53, Xwmc718, and Xwmc215 on chromosomes (e.g. 2D, 4A, and 5D) simultaneously controling grain yield and chlorophyll content, showed tight linkages or pleiotropisms. Three novel major QTLs, qGY5D, qChla5D, and qChlb5D, closely linked with the PCR marker Xwmc215 on chromosome 5D, accounted for 10.32%, 12.95%, and 23.29% of the phenotypic variance, respectively. The favorable alleles came from Yumai 57.
Keywordschlorophyll content grain yield marker-assisted selection quantitative trait loci Triticum aestivum L.
- Cao, W.D., Jia, J.Z., Jin, J.Y. 2004. Identification and interaction analysis of QTL for chlorophyll content in wheat seedlings. Plant Nutr. Ferti. Sci. 10:473–478.Google Scholar
- Fang, P., Yu, X.M., Zhu, R.Q., Wu, P. 2004. QTLs for rice leaf chlorophyll content under low N stress. Pedosphere 14:145–150.Google Scholar
- Guo, C.Q., Bai, Z.A., Liao, P.A., Jin, W.K. 2004. New high quality and yield wheat variety Yumai 57. China Seed Industry, 4:54.Google Scholar
- Hai, Y., Kang, M.H. 2007. Breeding of Huapei 3 new wheat variety with high yield and early maturing. Henan Agric. Sci. 5:36–37.Google Scholar
- Huang, X.Q., Chloutier, S., LyCad, L., Radovanovic, N., Humphreys, D.G., Noll, J.S., Somers, D.J., Brown, P.D. 2006. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor. Appl. Genet. 113:753–766.CrossRefGoogle Scholar
- Porra, R.J., Thompson, W.A., Kriedemann, P.E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta 975:384–394.CrossRefGoogle Scholar
- Shen, B., Zhuang, J.Y., Zhang, K.Q., Dai, W.M., Lu, Y., Fu, L.Q., Ding, J.M., Zheng, K.L. 2007. QTL mapping of chlorophyll content in rice. Agric. Sci. Sinic. 6:17–24.Google Scholar
- Yang, G., Li, S., Feng, L., Kong, J., Li, H., Li, Y. 2006. Analysis of QTL underlying the traits relative to the chlorophyll content of the flag leaf in rice. J. Wuhan Univ. 52:751–756.Google Scholar
- Zhang, Z.L. 1990. Guide to plant physiology experiments. Higher Education Press, Beijing.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.