Identification, gene postulation and molecular tagging of a stripe rust resistance gene in synthetic wheat CI142

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most serious diseases of wheat (Triticum aestivum L.) worldwide. Of 94 Triticum durum/Aegilops tauschii synthetic wheat accessions tested, CI142 (Garza/Boy//Ae. squarrosa 271) was found to be resistant to 6 Chinese PST races. The resistance to stripe rust in CI142 was proven to be controlled by a single dominant gene, tentatively designated YrC142. Gene postulation showed that the pathogenic specificity of CI142 is different from 21 other lines possessing known resistance genes, such as Yr10, Yr15, Yr24, and Yr26, located on chromosome 1B. Bulked segregant analysis (BSA) and F2 segregation analysis of the CI142/Mingxian 169 cross were used to analyse the SSR markers linked to YrC142. Five SSR markers were found to be closely associated with YrC142 in the order Xwmc419-YrC142-Xgwm273, Xbarc187-Xgwm18-Xwmc626, in which the relative genetic distances of these SSR loci to the gene YrC142 were 5.4, 0.8, 0.8, 1.0, and 2.4 cM, respectively. Two SSR markers (Xgwm273−162 and Xgwm18−168) distinguished YrC142 from Yr10, Yr15, Yr24, and Yr26, suggesting that these 2 SSR markers may be used as diagnostic ones for the gene in a wheat breeding program against stripe rust. Based on these findings, YrC142 is most likely a new gene or a new allele at the Yr26 locus, which provides an opportunity to diversify stripe rust-resistant resources for wheat breeding programs.

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Correspondence to Z. Y. Zhang.

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Wang, L.M., Zhang, Z.Y., Liu, H.J. et al. Identification, gene postulation and molecular tagging of a stripe rust resistance gene in synthetic wheat CI142. CEREAL RESEARCH COMMUNICATIONS 37, 209–215 (2009). https://doi.org/10.1556/CRC.37.2009.2.7

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Keywords

  • Triticum durum/Aegilops tauschii synthetic wheat
  • stripe rust
  • resistance gene
  • gene postulation
  • SSR marker