Tagging pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala
Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production. Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome.
Unable to display preview. Download preview PDF.
- Blaise, F., Rémy, E., Meyer, M., Zhou, L., Narcy, J.-P., Roux, J., Balesdent, M.H., Rouxel, T. 2007. A critical assessment of Agrobacterium tumefaciens -mediated transformation as a tool of pathogenicity gene discovery in the phytopathogenic fungus Leptosphaeria maculans. Fungal Genet. Biol. 44:123–138.CrossRefGoogle Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.