Advertisement

Cereal Research Communications

, Volume 36, Supplement 6, pp 217–222 | Cite as

Evaluating the ability of the barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes

  • Cahid Cakir
  • Steven R. ScofieldEmail author
Session 2 Genetics and Genomics in Fusarium Resistance

Abstract

Virus-induced gene silencing (VIGS) is an important tool for rapid assessment of gene function in plants. The ability of the Barley stripe mosaic virus (BSMV) VIGS system to simultaneously silence two genes was assessed by comparing the extent of down-regulation of the wheat PDS and SGT1 genes after wheat plants were infected with BSMV-VIGS constructs carrying 178bp fragments of the PDS and SGT1 genes linked in cis or after infection with single-gene silencing BSMV constructs carrying PDS or SGT1 alone or with a mixture of the single-gene constructs. Although significant variation occurred in these studies it was clearly demonstrated that two wheat genes can be silenced simultaneously by a single BSMV-VIGS construct.

Keywords

virus-induced gene silencing VIGS wheat functional genomics 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., Schulze-Lefert, P. 2002. The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance. Science 295:2073–2076.CrossRefGoogle Scholar
  2. Baulcombe, D.C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr Opin Plant Biol. 2:109–113.CrossRefGoogle Scholar
  3. Burch-Smith, T.M., Anderson, J.C., Martin, G.B., Dinesh-Kumar, S.P. 2004. Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J. 39:734–746.CrossRefGoogle Scholar
  4. Petty, I.T., Hunter, B.G., Wei, N., Jackson, A.O. 1989. Infectious barley stripe mosaic virus RNA transcribed in vitro from full-length genomic cDNA clones. Virology 171:342–349.CrossRefGoogle Scholar
  5. Pogue, G.P., Lindbo, J.A., Garger, S.J., Fitzmaurice, W.P. 2002. Making an ally from an enemy: plant virology and the new agriculture. Annu. Rev. Phytopathol. 40:45–74.CrossRefGoogle Scholar
  6. Scofield, S.R., Huang, L., Brandt, A.S., Gill, B.S. 2005. Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway. Plant Physiol. 138:2165–2173.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.USDA-ARS Crop Production and Pest Control UnitWest LafayetteUSA

Personalised recommendations