PCR Identification of Fusarium graminearum Isolated from Wheat Grain


Species-specific PCR assay was used for the identification of Hungarian Fusarium graminearum isolates in pure mycelial culture. The Fg16F/Fg16R primer pair of the three known species-specific primers appeared to be the most appropriate one to identify F. graminearum.

Two methods were used for comparative determination of the amplicon size of F. graminearum strains: traditional agarose gel electrophoresis, and chip electrophoresis. Our results have shown that the chip electrophoresis is an easy-to-use, time-efficient substitute for conventional agarose gel electrophoresis; moreover it provides a more precise size determination of amplicons. Amplicon size ranging from 415 bp to 421 bp in tested isolates may be associated with genetic diversity in the Hungarian population of F. graminearum.

The PCR assay described in this study can be used for the routine detection and identification of F. graminearum without isolation and morphological investigation of this fungus.


  1. Agilent 2100 Bioanalyzer. Application Compendium. 2005. Agilent Technologies. Printed in Germany, 95 pp.

  2. Binder, E.M. 2007. Managing the risk of mycotoxins in modern feed production. Animal Feed Science and Technology 133:149–166.

    CAS  Article  Google Scholar 

  3. Bottalico, A., Perrone, G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology 108:611–624.

    CAS  Article  Google Scholar 

  4. Burgess, L.W., Summerell, B.A., Bullock, S., Backhouse, D. 1994. Laboratory Manual for Fusarium Research. Third Edition, Sydney. 133 pp.

  5. Desjardins, A.E. 2006. Fusarium Mycotoxins. Chemistry, Genetics and Biology. APS Press, St. Paul, Minesota, USA. 260 pp.

    Google Scholar 

  6. Edwards, S.G., O’Callaghan, J., Dobson, D.W. 2002. PCR-based detection and quantification of mycotoxigenic fungi. Mycological Research 106:1005–1025.

    CAS  Article  Google Scholar 

  7. Jurado, M., Vázquez, C., Patino, B., González-Jaén, M.T. 2005. PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Systematic and Applied Microbiology 28:562–568.

    CAS  Article  Google Scholar 

  8. Láday, M., Juhász, Á., Mulé, G., Szécsi, Á., Logrieco, A. 2004. Mitochondrial DNA diversity and lineage determination of European isolates of Fusarium graminearum (Gibberella zeae). European Journal of Plant Pathology 110:545–550.

    Article  Google Scholar 

  9. Leslie, J.F., Summerell, B.A., Bullock, S. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, USA. 388 pp.

    Book  Google Scholar 

  10. Logrieco, A., Mulé, G., Moretti, A., Bottalico, A. 2002. Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe. European Journal of Plant Pathology 108:597–609.

    CAS  Article  Google Scholar 

  11. Miller, J.D., Trenholm, H.L. 1994. Mycotoxins in Grain. Compounds other than Aflatoxin. Eagon Press, St. Paul, Minesota, USA. 552 pp.

    Google Scholar 

  12. Mulé, G., Gonzalez-Jaén, M.T., Hornok, L., Nicholson, P., Waalwijk, C. 2005. Advances in molecular diagnosis of toxigenic Fusarium species. Food Additives and Contaminants 22:316–323.

    Article  Google Scholar 

  13. Nicholson, P., Simpson, D.R., Weston, G., Rezanoor, H.N., Lees. A.K., Parry, D.W., Joyce, D. 1998. Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 53:17–37.

    CAS  Article  Google Scholar 

  14. Nicholson, P., Chandler, E., Draeger, R. C., Gosman, N.E., Simpson, D. R., Thomsett, M., Wilson, A.H. 2003. Molecular tools to study epidemiology and toxicology of fusarium head blight of cereals. European Journal of Plant Pathology 109:691–703.

    CAS  Article  Google Scholar 

  15. O’Donnell, K., Kistler, H.C., Tacke, B.K., Casper, H.H. 2000. Gene genealogies reveal global phylogenetic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of National Academy of Sciences USA 97:7905–7910.

    Article  Google Scholar 

  16. O’Donnell, K., Ward, T.J., Geiser, D.M., Kistler, H.C., Aoki, T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genetics and Biology 41:600–623.

    Article  Google Scholar 

  17. Schilling, A.G., Möller, E.M., Geiger, H.H. 1996. Polymerase chain reaction-based assays for speicies-specific detection of Fusarium culmorum, Fusarium graminearum and Fusarium avenaceum. Phytopathology 86:515–522.

    CAS  Article  Google Scholar 

  18. Sudakin, D.L. 2003. Trichothecenes in the environment: relevance to human health. Toxicology Letters 143:97–107.

    CAS  Article  Google Scholar 

  19. Szécsi, Á., Mesterházy, Á. 1998. A medium for selective isolation and identification of Fusarium spp. from cereal grains and maize kernels. Acta Phytopathologica et Entomologica Hungarica 33:79–87.

    Google Scholar 

  20. Szécsi, Á., Bartók, T., Varga, M., Magyar, D., Mesterházy, Á. 2005. Determination of trichotecene chemotypes of Fusarium graminearum strains isolated in Hungary. Journal of Phytopathology 153:445–448.

    Article  Google Scholar 

  21. Waalwijk, C., Kastelein, P., deVries, I., Kerényi, Z., van der Lee, T., Hesselink, T., Köhl, J., Kema, G. 2003. Major changes in Fusarium spp. in wheat in the Netherlands. European Journal of Plant Pathology 109:743–754.

    CAS  Article  Google Scholar 

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Koncz, Z., Huszti, K., Naár, Z. et al. PCR Identification of Fusarium graminearum Isolated from Wheat Grain. CEREAL RESEARCH COMMUNICATIONS 36, 623–630 (2008). https://doi.org/10.1556/CRC.36.2008.4.11

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  • Fusarium graminearum
  • PCR
  • species-specific primers
  • chip electrophoresis
  • wheat grain