Identification and Localization of Molecular Markers Linked to the Lr52 Leaf Rust Resistance Gene of Wheat

Abstract

Growing resistant wheat cultivars is considered to be an efficient and environmentally safe approach in reducing damage caused by leaf rust disease. Among the numerous leaf rust resistance genes of wheat, Lr52 is a very effective one with a broad spectrum resistance. The objective of this study was to identify and map molecular markers closely linked to the Lr52 resistance gene. Out of 280 RAPD, 44 SSR and 8 STS markers tested, three showed close linkage to the Lr52. In our study, one SSR marker (Xwmc149) showed a close linkage (11.3 cM) to the Lr52 gene. Another SSR (Xgwm234) and an STS (Xtxw200) markers, having close linkage to a recently identified leaf rust resistance gene found in PI 289824, also showed a close linkage (7.2 and 3.6 cM, respectively) to the Lr52. Matchings in the mapping distances of these markers linked to these resistance genes still remain open the possibility that the gene found in PI 289824 is identical to the Lr52, or simply this locate very close to it.

References

  1. Dyck, P.L., Jedel, P.E. 1989. Genetics of resistance to leaf rust in two accessions of common wheat. Can. J. Plant Sci. 69:531–534.

    Article  Google Scholar 

  2. Guyomarc’h, H., Sourdille, P., Charmet, G., Edwards, K., Bernard, M. 2002. Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor. Appl. Genet. 104:1164–1172.

    Article  Google Scholar 

  3. Gupta, K., Balyan, S., Edwards, J., Isaac, P., Korzun, V., Röder, M.S., Gautier, M.F., Joudrier, P., Schlatter, R., Dubcovsky, J., De La Pena, C., Khairallah, M., Penner, G., Hayden, J., Sharp, P., Keller, B., Wang, C., Hardouin, P., Jack, P., Leroy, P. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor. Appl. Genet. 105:413–422.

    CAS  Article  Google Scholar 

  4. Hiebert, C., Thomas, J., McCallum, B. 2002. Determining the chromosomal location of the wheat leaf-rust resistance gene LrW. Can. J. Plant Pathol 24:92–94.

    Article  Google Scholar 

  5. Hiebert, C., Thomas, J., McCallum, B. 2005. Locating the broadspectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method. Theor. Appl. Genet. 110:1453–1457.

    CAS  Article  Google Scholar 

  6. Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12:172–175.

    Article  Google Scholar 

  7. Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daley, M., Lincoln, S., Newburg, L. 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181.

    CAS  Article  Google Scholar 

  8. McIntosh, R.A., Yamazaki, Y., Devos, K.M., Dubcovsky, J., Rogers, W.J., Appels, R. 2003. Catalogue of gene symbols for wheat. https://doi.org/wheat.pw.usda.gov/ggpages/wgc/2003/

  9. McIntosh, R.A., Devos, K.M., Dubcovsky, J., Rogers, W.J., Morris, C.F., Appels, R., Somers, D.J., Anderson, D.J. 2007. V. Catalogue of gene symbols for wheat: 2007 supplement. https://doi.org/wheat.pw.usda.gov/ggpages/awn/53/Textfile/WGC.html

  10. Obert, D.E., Fritz, A.K., Moran, J.L., Singh, S., Rudd, J.C., Menz, M.A. 2005. Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat Theor. Appl. Genet. 110:1439–1444.

    CAS  Article  Google Scholar 

  11. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.

    PubMed  PubMed Central  Google Scholar 

  12. Rogers, S.O., Bendich, A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissue. Plant Mol. Biol. 5:69–76.

    CAS  Article  Google Scholar 

  13. Sayre, K.D., Singh, R.P., Huerta-Espino, J., Rajaram, S. 1998. Genetic progress in reducing losses to leaf rust in CIMMYT-derived Mexican spring wheat cultivars. Grop Science 38:654–659.

    Google Scholar 

  14. Schachermayr, G., Messmer, M., Feuillet, C., Winzeler, H., Winzeler, M., Keller, B. 1995. Identification of molecular markers linked to the Agropyron elongatum -derived leaf rust resistance gene Lr24 in wheat. Theor. Appl. Genet. 90:982–990.

    CAS  Article  Google Scholar 

  15. Somers, D.J., Isaac, P., Edwards, K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.

    CAS  Article  Google Scholar 

  16. Song, Q.J., Shi, J.R., Singh, S., Fickus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.G. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110:550–560.

    CAS  Article  Google Scholar 

  17. Stakman, E.C., Stewart, D.M., Loegering, W.Q. 1962. Identification of physiologic races of Puccinia graminis var. tritici. Agricultural Research Service E617. (United States Department of Agriculture: Washington D.C.)

    Google Scholar 

  18. Thomas, J., Riedel, E., Penner, G. 2001. An efficient method for assigning traits to chromosomes. Euphytica 119:217–221.

    CAS  Article  Google Scholar 

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Correspondence to L. Purnhauser.

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Tar, M., Purnhauser, L. & Csősz, M. Identification and Localization of Molecular Markers Linked to the Lr52 Leaf Rust Resistance Gene of Wheat. CEREAL RESEARCH COMMUNICATIONS 36, 409–415 (2008). https://doi.org/10.1556/CRC.36.2008.3.5

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Keywords

  • gene mapping
  • LrW
  • microsatellite
  • Puccinia triticina
  • RAPD
  • SSR
  • STS
  • Triticum aestivum