Cereal Research Communications

, Volume 36, Issue 3, pp 409–415 | Cite as

Identification and Localization of Molecular Markers Linked to the Lr52 Leaf Rust Resistance Gene of Wheat

  • M. Tar
  • L. PurnhauserEmail author
  • M. Csősz


Growing resistant wheat cultivars is considered to be an efficient and environmentally safe approach in reducing damage caused by leaf rust disease. Among the numerous leaf rust resistance genes of wheat, Lr52 is a very effective one with a broad spectrum resistance. The objective of this study was to identify and map molecular markers closely linked to the Lr52 resistance gene. Out of 280 RAPD, 44 SSR and 8 STS markers tested, three showed close linkage to the Lr52. In our study, one SSR marker (Xwmc149) showed a close linkage (11.3 cM) to the Lr52 gene. Another SSR (Xgwm234) and an STS (Xtxw200) markers, having close linkage to a recently identified leaf rust resistance gene found in PI 289824, also showed a close linkage (7.2 and 3.6 cM, respectively) to the Lr52. Matchings in the mapping distances of these markers linked to these resistance genes still remain open the possibility that the gene found in PI 289824 is identical to the Lr52, or simply this locate very close to it.


gene mapping LrW microsatellite Puccinia triticina RAPD SSR STS Triticum aestivum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Dyck, P.L., Jedel, P.E. 1989. Genetics of resistance to leaf rust in two accessions of common wheat. Can. J. Plant Sci. 69:531–534.CrossRefGoogle Scholar
  2. Guyomarc’h, H., Sourdille, P., Charmet, G., Edwards, K., Bernard, M. 2002. Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor. Appl. Genet. 104:1164–1172.CrossRefGoogle Scholar
  3. Gupta, K., Balyan, S., Edwards, J., Isaac, P., Korzun, V., Röder, M.S., Gautier, M.F., Joudrier, P., Schlatter, R., Dubcovsky, J., De La Pena, C., Khairallah, M., Penner, G., Hayden, J., Sharp, P., Keller, B., Wang, C., Hardouin, P., Jack, P., Leroy, P. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor. Appl. Genet. 105:413–422.CrossRefGoogle Scholar
  4. Hiebert, C., Thomas, J., McCallum, B. 2002. Determining the chromosomal location of the wheat leaf-rust resistance gene LrW. Can. J. Plant Pathol 24:92–94.CrossRefGoogle Scholar
  5. Hiebert, C., Thomas, J., McCallum, B. 2005. Locating the broadspectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method. Theor. Appl. Genet. 110:1453–1457.CrossRefGoogle Scholar
  6. Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12:172–175.CrossRefGoogle Scholar
  7. Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daley, M., Lincoln, S., Newburg, L. 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181.CrossRefGoogle Scholar
  8. McIntosh, R.A., Yamazaki, Y., Devos, K.M., Dubcovsky, J., Rogers, W.J., Appels, R. 2003. Catalogue of gene symbols for wheat.
  9. McIntosh, R.A., Devos, K.M., Dubcovsky, J., Rogers, W.J., Morris, C.F., Appels, R., Somers, D.J., Anderson, D.J. 2007. V. Catalogue of gene symbols for wheat: 2007 supplement.
  10. Obert, D.E., Fritz, A.K., Moran, J.L., Singh, S., Rudd, J.C., Menz, M.A. 2005. Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat Theor. Appl. Genet. 110:1439–1444.CrossRefGoogle Scholar
  11. Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.PubMedPubMedCentralGoogle Scholar
  12. Rogers, S.O., Bendich, A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissue. Plant Mol. Biol. 5:69–76.CrossRefGoogle Scholar
  13. Sayre, K.D., Singh, R.P., Huerta-Espino, J., Rajaram, S. 1998. Genetic progress in reducing losses to leaf rust in CIMMYT-derived Mexican spring wheat cultivars. Grop Science 38:654–659.Google Scholar
  14. Schachermayr, G., Messmer, M., Feuillet, C., Winzeler, H., Winzeler, M., Keller, B. 1995. Identification of molecular markers linked to the Agropyron elongatum -derived leaf rust resistance gene Lr24 in wheat. Theor. Appl. Genet. 90:982–990.CrossRefGoogle Scholar
  15. Somers, D.J., Isaac, P., Edwards, K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.CrossRefGoogle Scholar
  16. Song, Q.J., Shi, J.R., Singh, S., Fickus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.G. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110:550–560.CrossRefGoogle Scholar
  17. Stakman, E.C., Stewart, D.M., Loegering, W.Q. 1962. Identification of physiologic races of Puccinia graminis var. tritici. Agricultural Research Service E617. (United States Department of Agriculture: Washington D.C.)Google Scholar
  18. Thomas, J., Riedel, E., Penner, G. 2001. An efficient method for assigning traits to chromosomes. Euphytica 119:217–221.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2008

Authors and Affiliations

  1. 1.Cereal Research Non-Profit CompanySzegedHungary

Personalised recommendations