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Cereal Research Communications

, Volume 36, Issue 3, pp 477–487 | Cite as

Characterization of HMW-GS Genes Dx5t and Dy12t from Aegilops tauschii Accession with Subunit Combination Dx5t + Dy12t

  • Z. H. YanEmail author
  • Z. F. Guo
  • D. C. Liu
  • S. F. Dai
  • Y. M. Wei
  • Y. L. Zheng
Open Access
Article

Abstract

Aegilops tauschii is the generally accepted D genome diploid donor of hexaploid wheat. The significance of Ae. tauschii HMW-GS genes on bread-making properties of bread wheat has been well documented. Among them, Ae. tauschii HMW-GS Dx5t+Dy12t was thought as the pair with potentially value in endowing synthetic hexaploid wheat with good end-use qualities. In this paper, we isolated and sequenced genes Dx5t and Dy12t from Ae. tauschii accession As63. Amino acid sequence comparison indicated that Dy12t from Ae. tauschii is more similar to Dy10 rather than Dy12 of bread wheat. The sequence of Dx5t in Ae. tauschii accession As63 showed higher similarity to that of Dx5 in bread wheat than others. However, it is notable that Dx5t lacked the additional cysteine residue in Dx5, which is responsible for good bread-making quality in common wheat. Moreover, compared to Dx5, Dx5t has an extra hexpeptide repetitive motif unit (SGQGQQ) as well as five amino acid substitutions.

Keywords

Ae. tauschii HMW-GS sequence analysis 

References

  1. Anderson, O.D., Békés, F., Gras, P.W., Khul, J., Tam, A. 1996. HMW glutenins: Structure function relationships step by step. In: Wrigley, C.W. (ed.), Proc. 6 th Intern. Workshop on Gluten Proteins, pp. 195–198.Google Scholar
  2. Belton, P.S. 1999. On the elasticity of wheat gluten. J. Cereal Sci. 29:103–107.CrossRefGoogle Scholar
  3. Békés, F., Anderson, O.D., Gras, P.W., Gupta R.B., Tam, A., Wrigley, C.W., Appels, R. 1994. The contribution to mixing properties of 1D HMW glutenin subunits expressed in a bacterial system. In: Henry, R.J., Ronalds, J.A. (eds.), Improvement of Cereal Quality by Genetic Engineering. Plenum Press, New York, pp. 97–103.CrossRefGoogle Scholar
  4. Branlard, G., Dardevet, M. 1985. Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J. Cereal Sci. 3:345–354.CrossRefGoogle Scholar
  5. Campbell, W.P., Wrigley, C.W., Cressey, P.J., Slack, C.R. 1987. Statistical correlations between quality attributes and grain protein composition for 71 hexaploid wheats used as breading parents. Cereal Chem. 65:293–299.Google Scholar
  6. Cressey, P.J., Campbell, W.P., Wrigley, C.W., Griffin, W. 1987. Statistical correlation between quality attributes and grain protein composition for 60 advanced lines of crossed bred wheat. Cereal Chem. 64:299–301.Google Scholar
  7. Feeney, K.A., Wellner, N., Gilbert, S.M., Halford, N.G., Tatham, A.S., Shewry, P.R., Belton, P.S. 2003. Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolym. Biospectrosc. 72:123–131.CrossRefGoogle Scholar
  8. Hsam, S.L.K., Kieffer, R., Zeller, F.J. 2001. Significance of Aegilops tauschii glutenin genes on breadmaking properties of wheat. Cereal Chem. 78:521–525.CrossRefGoogle Scholar
  9. Kihara, H. 1944. Discovery of the DD-analyser, one of the ancestors of Triticum vulgare. Agric. Hortic. 19:13–14.Google Scholar
  10. Kumar, S., Tamura, K., Jakobsen, B.I., Nei, M. 2001. MEGA2: Molecular evolutionary genetics analysis software. Arizona State University, Tempe, Arizona, USA.Google Scholar
  11. Kreis, M., Shewry, P.R., Forde, B.G., Miflin, B.J. 1985. Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barely and rye. Oxford Surv. Plant Mol. Cell Boil. 2:253–317.Google Scholar
  12. Lafiandra, D., D’ovidio, R., Proceddu, E., Margiotta, B., Colaprico, G. 1993. New data supporting high Mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12. J. Cereal Sci. 18:197–205.CrossRefGoogle Scholar
  13. Lagudah, E.S., Brienl, O., Halloran, G.M. 1988. Influence of gliadin composition and high molecular weight subunit of glutenin on dought properties in an F 3 population of a bread wheat cross. J. Cereal Sci. 7:33–42.CrossRefGoogle Scholar
  14. Lagudah, E.S., Halloran, G.M. 1988. Phylogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat. I variation in HMW subunits of glutenin and gliadins. Theor. Appl. Genet. 75:592–598.CrossRefGoogle Scholar
  15. Lagudah, E.S., Macritchie, F., Halloran, G.M. 1987. The influence of high-molecular-weight subunits of glutenin from Triticum tauschii on four quality of synthetic hexaploid wheat. J. Cereal Sci. 5:129–138.CrossRefGoogle Scholar
  16. Lawrence, G.J., Shepherd, K.W. 1981. Chromosomal location of genes controlling seed protein in species related to wheat. Theor. Appl. Genet. 59:25–31.CrossRefGoogle Scholar
  17. Lorenzo, A., Kronstad, W.E., Vieira, L.C.E. 1987. Relationship between high molecular weight glutenin subunit and loaf volume in wheat as measured by the Sodium dodecyl sulfate sedimentation test. Crop Sci. 27:253–257.CrossRefGoogle Scholar
  18. Machie, A.M., Lagudah, E.S., Sharp, P.J., Lafiandra, D. 1996a. Molecular and biochemical characterization of HMW-GSs from T. tauschii and the D genome of hexaploid wheat. J. Cereal Sci. 23:213–225.CrossRefGoogle Scholar
  19. Machie, A.M., Sharp, P.J., Lagudah, E.S. 1996b. The nucleotide and derived amino acid sequence of a HMW-GS gene from Triticum tauschii and comparison with those from the D genome of bread wheat. J. Cereal Sci. 24:73–78.CrossRefGoogle Scholar
  20. McFadden, E.S., Sears, E.R. 1946. The origin of Triticum spelta and its free-threshing hexaploid relatives. J. Hered. 37:81–89, 107–116.CrossRefGoogle Scholar
  21. Ng, P.K.W., Bushuk, W. 1988. Statistical relationship between high molecular weight subunits of glutenin and bread making quality of Canadian-grown wheat. Cereal Chem. 65: 408–413.Google Scholar
  22. Payne, P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu. Rev. Plant Physiol. 38:141–153.CrossRefGoogle Scholar
  23. Payne, P.I., Harris, P.A., Law, C.N., Holt, L.M., Blackman, J.A. 1980a. The high molecular-weight subunits of glutenin: structure, genetics and relationship to bread-making quality. Annu. Technol. Agr. 29:309–320.Google Scholar
  24. Payne, P.I., Law, C.N., Mudd, E.E. 1980b. Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 58:113–120.CrossRefGoogle Scholar
  25. Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex gene loci, Glu-Al, Glu-Bl and Glu-Dl which code for high molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.Google Scholar
  26. Peña, R.J., Zarco-Hernandez, J., Mujeeb-Kazi, A. 1995. Glutenin subunit compositions and bread-making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum × Triticum tauschii (coss.) Schmal crosses. J. Cereal Sci. 21:15–23.CrossRefGoogle Scholar
  27. Pogna, N.E., Mellini, F., Dal Belin Peruffo, A. 1987. Glutenin subunits of Italian common wheats of good bread making quality and comparative effects of the 2 and 5, 10 and 12 on flour quality. In: Borghi, B. (ed.), Agriculture hard wheat agronomic technological, biochemical and genetical aspects Commission of the European community CEC Rep ECR 1172 EN, Brrussels pp. 53–69.Google Scholar
  28. Shewry, P.R., Halford, N.G., Tatham, A.S., Popineau, Y., Lafiandra, D., Belton, P.S. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv. Food Nutr. Res. 45:221–302.Google Scholar
  29. Weegels, P.L., Hamer, R.J., Schofield, J.D. 1996. Critical review: Functional properties of wheat glutenin. J. Cereal Sci. 23:1–18.CrossRefGoogle Scholar
  30. William, M.D.H.M., Peña, R.J., Mujeeb-kazi, A. 1993. Seed protein and isozyme variations in Triticum tauschii (Aegiloips squarrosa). Theor. Appl. Genet. 87:257–263.CrossRefGoogle Scholar
  31. Wrigley, C.W., Békés, F., Cavanagh, C.R., Martinov, S., Bushuk, S. 2006. The Gluten Composition of Wheat Varieties and Genotypes. PART II. Composition table for the HMW subunits of glutenin. (https://doi.org/www.aaccnet.org)
  32. Yan, Y.M., Hsan, S.L.K., Yu, J.Z., Jiang, Y., Zeller, F.J. 2003. Allelic variation of the HMW-GSs in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis. Euphytica 130:377–385.CrossRefGoogle Scholar
  33. Yan, Z.H. 2005. Molecular cloning of high molecular weight glutenin subunit genes from Aegilops species. Higher Education Press, Beijing, China, pp. 26–33.Google Scholar
  34. Yan, Z.H., Wan, Y.F., Liu, K.F., Zheng, Y.L., Wang, D.W. 2002. Identification of a novel HMW-GS and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin 47:220–225.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Z. H. Yan
    • 1
    Email author
  • Z. F. Guo
    • 1
    • 2
  • D. C. Liu
    • 1
  • S. F. Dai
    • 1
  • Y. M. Wei
    • 1
  • Y. L. Zheng
    • 1
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityDujiangyan CityChina
  2. 2.Shenyang Agricultural UniversityShenyang CityChina

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