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Cereal Research Communications

, Volume 36, Issue 2, pp 235–246 | Cite as

Validation and Utilisation of Rht Dwarfing Gene Specific Markers

  • E. G. Pestsova
  • V. Korzun
  • A. BörnerEmail author
Article

Abstract

PCR assays specific for the GA insensitive dwarfing genes (alleles) Rht-B1b and Rht-D1b were employed to study a series of additional alleles of Rht-B1 and Rht-D1. The amplification profiles of Rht-B1b and Rht-B1d were not distinguishable from one another, whereas lines carrying Rht-B1c, Rht-B1e and Rht-B1f amplified a product analogous to that of the wild type. At the 4D locus, no discrimination was possible between Rht-D1b, Rht-D1c and Rht-D1d. As a result, the utilisation of these PCR assays is limited. Examples of the analysis of germplasm and spontaneous occurring off-types are presented.

Keywords

wheat GA insensitivity reduced height isogenic lines off-types PCR markers genebank collection 

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Copyright information

© Akadémiai Kiadó, Budapest 2008

Authors and Affiliations

  1. 1.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany
  2. 2.Lochow-Petkus GmbHBergenGermany

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