Validation and Utilisation of Rht Dwarfing Gene Specific Markers

Abstract

PCR assays specific for the GA insensitive dwarfing genes (alleles) Rht-B1b and Rht-D1b were employed to study a series of additional alleles of Rht-B1 and Rht-D1. The amplification profiles of Rht-B1b and Rht-B1d were not distinguishable from one another, whereas lines carrying Rht-B1c, Rht-B1e and Rht-B1f amplified a product analogous to that of the wild type. At the 4D locus, no discrimination was possible between Rht-D1b, Rht-D1c and Rht-D1d. As a result, the utilisation of these PCR assays is limited. Examples of the analysis of germplasm and spontaneous occurring off-types are presented.

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Correspondence to A. Börner.

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Pestsova, E.G., Korzun, V. & Börner, A. Validation and Utilisation of Rht Dwarfing Gene Specific Markers. CEREAL RESEARCH COMMUNICATIONS 36, 235–246 (2008). https://doi.org/10.1556/CRC.36.2008.2.4

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Keywords

  • wheat
  • GA insensitivity
  • reduced height
  • isogenic lines
  • off-types
  • PCR markers
  • genebank collection