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Cereal Research Communications

, Volume 36, Issue 2, pp 225–234 | Cite as

Molecular, Serological and Transmission Electron Microscopic Analysis of the Barley Yellow Dwarf Virus-PAV and the Cereal Yellow Dwarf Virus-RPV in Canary Seed (Phalaris canariensis L.)

  • H. Ilbagi
  • F. Rabenstein
  • A. Habekuss
  • F. Ordon
  • A. Citir
  • O. Cebeci
  • H. BudakEmail author
Open Access
Article

Abstract

Canary seed (Phalaris canariensis L.) is a cereal crop belonging to the tribe Phalarideae of the Poaceae family. A prevailing virus infection, which causes dwarfing of plants and yellowing of leaves, was observed in canary seed fields in the Tekirdag province of Turkey. The aim of this study was to identify, clone and sequence the cereal viruses naturally occurring on P. canariensis by employing serological tests as DAS-ELISA and TAS-ELISA tests combined with transmission electron microcopy (TEM) and molecular analysis. One hundred and one plant samples showing symptoms were collected and tested serologically using polyclonal antisera against Barley yellow dwarf virus -PAV (BYDV-PAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV). The results of both immunoassays showed that 48% of the samples were infected with BYDV-PAV, 2% with CYDV-RPV and 14% were mixed infection. 36% of the samples were uninfected or infected at level below detection. Aphid transmission experiments revealed that barley (cv. Rubina) exhibited characteristic of CYDV-RPV. Investigations of infected canary grass seedlings using transmission electron microscopy (TEM) supported the findings of serological tests and revealed the presence of isometric particles of approximately 25 nm in diameter. These results were also confirmed by using BYDV-PAV and CYDV-RPV specific primers. Sequence analysis of cDNA and probable translation products revealed a high level of homology to BYDV-PAV and CYDV-RPV isolates found in other plant species. The sequence data obtained from this research were deposited in the EMBL/GenBank Data Libraries under the accession nos. EGO19056 and EF372272.

Keywords

cereals viruses cDNA DAS-ELISA TAS-ELISA 

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Copyright information

© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • H. Ilbagi
    • 1
  • F. Rabenstein
    • 2
  • A. Habekuss
    • 3
  • F. Ordon
    • 3
  • A. Citir
    • 1
  • O. Cebeci
    • 4
  • H. Budak
    • 4
    Email author
  1. 1.Department of Plant Protection, Faculty of AgricultureNamık Kemal UniversityTekirdagTurkey
  2. 2.Federal Centre for Breeding Research on Cultivated PlantsInstitute of Resistance Research and Pathogen DiagnosticsQuedlinburgGermany
  3. 3.Federal Centre for Breeding Research on Cultivated PlantsInstitute of Epidemiology and Resistance ResourcesQuedlinburgGermany
  4. 4.Sabanci University Biological Sciences and Bioengineering ProgramIstanbulTurkey

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