Combined Effect of Water Stress and Infection with the Necrotrophic Fungal Pathogen Drechslera tritici-repentis on Growth and Antioxidant Activity in Wheat

Abstract

Treatment with various concentrations (0, 5, 15 and 20%) of PEG was used to simulate water stress, followed by inoculation with Drechslera tritici-repentis (DTR) at two different points of time (6 and 72 h after the PEG treatment) in two DTR resistant (M-3 and Mv Magvas) and two sensitive (Bezostaya 1 and Glenlea) wheat varieties. The reduction in biomass production due to the PEG treatments was more pronounced in the shoots than in the roots. While in the case of Bezostaya 1 5% PEG reduced the level of infection, 20% PEG treatment lowered the tolerance level of M-3. DTR infection may be more efficient in inducing antioxidative defence systems than water stress. However, there was no direct correlation between the activity of the individual antioxidant enzymes and the drought or DTR tolerance of wheat plants.

References

  1. Ádám, A., Bestwick, C.S., Barna, B., Mansfield, J.W. 1995. Enzymes regulating the accumulation of active oxygen species during the hypersensitive reaction of bean to Pseudomonas syringae pv. phaseolica. Planta 197:240–249.

    Article  Google Scholar 

  2. Blum, A. 1988. Plant Breeding for Stress Environments. CRC Press, Boca Raton, pp. 15–24.

    Google Scholar 

  3. Bridger, G.M., Yang, W., Falk, D.E., McKersie, B.D. 1994. Cold acclimation increases tolerance of activated oxygen in winter cereals. J. Plant Physiol. 144:235–240.

    CAS  Article  Google Scholar 

  4. Danna, C.H., Bartoli, C.G., Sacco, F., Ingala, L.R., Santa-María, G.E., Guiamet, J.J., Ugalde, R.A. 2003. Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity. Plant Physiol. 132:2116–2125.

    CAS  Article  Google Scholar 

  5. De Wolf, E.D., Effertz, R.J., Ali, S., Francl, L.J. 1998. Vistas of tan spot research. Canadian J. Plant Pathol. 20:349–444.

    Article  Google Scholar 

  6. Duveiller, E., Dubin, H.J., Reeves, J., McNab, A. 1997. Helminthosporium blights of wheat: spot blotch and tan spot. Proceedings of an International Workshop held at CIMMYT, El Batan, Mexico.

  7. El-Tayeb, M.A. 2006. Differential response of two Vicia faba cultivars to drought: growth, pigments, lipid peroxidation, organic solutes, catalase and peroxidase activity. Acta Agron. Hung. 54:25–37.

    CAS  Article  Google Scholar 

  8. Gaudet, D.A., Laroche, A., Frick, M., Huel, R., Puchalski, B. 2003. Plant development affects the cold-induced expression of plant defence-related transcripts in winter wheat. Physiol. Mol. Plant Pathol. 62:175–184.

    CAS  Article  Google Scholar 

  9. Hoagland, D.R., Arnon, D.I. 1950. The water-culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ. 347:1036–1043.

    Google Scholar 

  10. Hoffmann, B., Burucs, Z. 2005. Adaptation of wheat (Triticum aestivum L.) genotypes and related species to water deficiency. Cereal Res. Comm. 33:681–687.

    Article  Google Scholar 

  11. Janda, T., Szalai, G., Tari, I., Páldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays L.) plants. Planta 208:175–180.

    CAS  Article  Google Scholar 

  12. Janda, T., Szalai, G., Rios-Gonzalez, K., Veisz, O., Páldi, E. 2003. Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. 164:301–306.

    CAS  Article  Google Scholar 

  13. Janda, T., Kósa, E., Pintér, J., Szalai, G., Marton, C.L., Páldi, E. 2005. Antioxidant activity and chilling tolerance of young maize inbred lines and their hybrids. Cereal Res. Comm. 33:541–548.

    CAS  Article  Google Scholar 

  14. Janda, T., Szalai, G., Leskó, K., Yordanova, R., Apostol, S., Popova, L.P. 2007. Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light. Phytochemistry 68:1674–1682.

    CAS  Article  Google Scholar 

  15. Kocsy, G., Galiba, G., Brunold, C. 2001. Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol. Plant. 113:158–164.

    CAS  Article  Google Scholar 

  16. Kovács, G., Sorvari, S., Scott, P., Toldi, O. 2007. Pyrophosphate:fructose 6-phosphate 1-phosphotransferase is involved in the mobilization of sugar reserves in the taproots of cold- and drought-stressed carrot plants. Acta Agron. Hung. 55:71–82.

    Article  Google Scholar 

  17. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405–410.

    CAS  Article  Google Scholar 

  18. Murray, T.D., Parry, D.W., Cattlin, N.D. 1998. A Colour Handbook of Diseases of Small Grain Cereal Crops. Manson Publishing Ltd, London.

    Google Scholar 

  19. Sanchez-Urdaneta, A.B., Pena-Valdivia, C.B., Trejo, C., Aguirre, J.R., Cardenas, E. 2005. Root growth and proline content in drought sensitive and tolerant maize (Zea mays L.) seedlings under different water potentials. Cereal Res. Comm. 33:697–704.

    Article  Google Scholar 

  20. Sarker, A.M., Rahman, M.S., Paul, N.K. 1999. Effect of soil moisture on relative leaf water content, chlorophyll, proline and sugar accumulation in wheat. J. Agron. Crop Sci. 183:225–229.

    CAS  Article  Google Scholar 

  21. Smith, I.K., Vierheller, T.L., Thorne, C.A. 1988. Assay of glutathione reductase in crude tissue homogenates using 5,5-dithiobis(2-nitrobenzoic acid). Anal. Biochem. 175:408–413.

    CAS  Article  Google Scholar 

  22. Stubbs, R.W., Prescot, J.M., Sarri, E.E., Dubin, H.J. 1986. Cereal Dis. Method. Manual. CIMMYT, Mexico, pp. 1–46.

    Google Scholar 

  23. Yordanov, I., Velikova, V., Tsonev, T. 2000. Plant responses to drought, acclimation, and stress tolerance. Photosyntetica 38:171–186.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to T. Janda.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Janda, T., Cséplő, M., Németh, C. et al. Combined Effect of Water Stress and Infection with the Necrotrophic Fungal Pathogen Drechslera tritici-repentis on Growth and Antioxidant Activity in Wheat. CEREAL RESEARCH COMMUNICATIONS 36, 53–64 (2008). https://doi.org/10.1556/CRC.36.2008.1.6

Download citation

Keywords

  • abiotic stress
  • antioxidant enzymes
  • biotic stress
  • drought
  • tan spot
  • Triticum aestivum L.