Cereal Research Communications

, Volume 36, Issue 1, pp 43–51 | Cite as

Effect of Dicamba on Wheat Haploid Embryo Development

  • S. ProdanovicEmail author
  • F. Matzk
  • D. Zoric


An interspecies zygote derives after wheat × maize crosses. This zygote aborts in absence of auxin stimulation. Treatment with dicamba is widely used to promote wheat haploid embryo development. We analyzed the effect of dicamba on wheat haploid embryo development. Four treatments related to the time of dicamba treatment were carried out: 1 day before pollination and 1, 5 and 9 days after pollination. It was concluded that wheat haploid embryos developed only in spikes treated with dicamba one or five days after pollination with maize. Dicamba treatment of wheat spikes prior to pollination hinders zygote formation and embryo development. Dicamba treatment 9 days after pollination is too late because young embryos have already aborted.


dicamba embryo haploid wheat × maize 


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  1. Campbell, A.W., Griffin, W.B., Burritt, D.J., Conner, A.J. 2001. The importance of light intensity for pollen tube growth and embryo survival in wheat × maize crosses. Annals of Botany 87:517–522.CrossRefGoogle Scholar
  2. Filippov, M., Miroshnichenko, D., Vernikovskaya, D., Dolgov, S. 2006. The effect of auxins, time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat. Plant Cell, Tissue and Organ Culture 84:100192–100201.CrossRefGoogle Scholar
  3. Fischer-Iglesias, C., Sundberg, B., Neuhaus, G., Jones, A.M. 2001. Auxin distribution and transport during embyonic pattern formation in wheat. Plant J. 26:115–129.CrossRefGoogle Scholar
  4. Gernand, D., Rutten, T., Varshney, A., Rubtsova, M., Prodanovic, S., Bruess, C., Kumlehn, J., Matzk, F., Houben A. 2005. Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatization and DNA fragmentation. Plant Cell 17:2431–2438.CrossRefGoogle Scholar
  5. Inagaki, M., Tahir, M. 1990. Comparison of haploid production frequencies in wheat varieties crossed with Hordeum bulbosum L. and maize. Jap. J. Breed. 40:165–174.CrossRefGoogle Scholar
  6. Kaushik, N., Sirohi, M., Khanna, V.K. 2004. Influence of age of the embryo and method of hormone application on haploid embryo formation in wheat × maize crosses. Proceedings of the 4th International Crop Science Congress. Brisbane, Australia, 26 Sep–1 Oct 2004. p. 771.Google Scholar
  7. Kisana, N.S., Nkongolo, K.K., Quick, J.S., Johnson, D.L. 1993. Production of double haploids by anther culture and wheat × maize method in wheat breeding programme. Plant Breeding 110:96–102.CrossRefGoogle Scholar
  8. Knox, R.E., Clarke, J.M., DePauw, R.M. 2000. Dicamba and growth condition effects on doubled haploid production in durum wheat crossed with maize. Plant Breeding 119:289–298.CrossRefGoogle Scholar
  9. Kumlehn, J., Lorz, H., Kranz, E. 1999. Monitoring individual development of isolated wheat zygotes: a novel approach to study early embryogenesis. Protoplasma 208:156–162.CrossRefGoogle Scholar
  10. Laurie, D.A., Bennett, M.D. 1986. Wheat × maize hybridization. Can. J. Genet. Cytol. 28:313–316.CrossRefGoogle Scholar
  11. Laurie, D.A., Bennett, M.D. 1988. The production of haploid wheat plants from wheat × maize crosses. Theor. Appl. Genet. 76:393–397.CrossRefGoogle Scholar
  12. Lefebvre, D., Devaux, P. 1996. Doubled haploid of wheat from wheat × maize crosses: genotypic influence, fertility and inheritance of the 1BL-1RS chromosome. Theor. Appl. Genet. 93: 1267–1273.CrossRefGoogle Scholar
  13. Magalhaes, A.C., Ashton, F.M. 1969. Effect of dicamba on oxygen uptake and cell membrane permeability in leaf tissue of Cyperus rotundus L. Weed Research 9:48–52.CrossRefGoogle Scholar
  14. Martinez, M., Cuadrado, C., Laurie, D.A., Romero, C. 2005. Synaptic behaviour of hexaploid wheat haploids with different effectiveness of the diploidizing mechanism. Cytogenetic and Genome Research 109:210–214.CrossRefGoogle Scholar
  15. Matzk, F., Mahn, A. 1994. Improved techniques for haploid production in wheat using chromosome elimination. Plant Breeding 113:125–129.CrossRefGoogle Scholar
  16. Mehta, Y.R., Angra, D.C. 2000. Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat × maize hybrids. Genet. Mol. Biol. (Sao Paulo) 23:617–622.CrossRefGoogle Scholar
  17. Mochida, K., Tsujimoto, H., Sasakuma, T. 2004. Confocal analysis of chromosome behavior in wheat × maize zygotes. Genome 47:199–205.CrossRefGoogle Scholar
  18. Rines, H.W., Dahleen, L.S. 1990. Haploids of plants produced by application of maize pollen to emasculated oat florets. Crop Science 30:1073–1078.CrossRefGoogle Scholar
  19. Rober-Kleber, N., Albrechtova, J.T.P., Fleig, S., Huck, N., Michalke, W., Wagner, E., Speth, V., Neuhaus, G., Fisher-Iglesias, C. 2003. Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development. Plant Physiol. 131:1–11.CrossRefGoogle Scholar
  20. Satyavathi, V.V., Jauhar, P.P., Elias, E., Rao, M.B. 2004. Effect of growth regulators on in vitro plant regeneration in durum wheat. Crop Science 44:1839–1846.CrossRefGoogle Scholar
  21. Savaskan, C., Ellerbrook, C., Fish, L.S., Snape, J.W. 1997. Doubled haploid production in Turkish durum wheats using crosses with maize. Plant Breeding 116:299–301.CrossRefGoogle Scholar
  22. Sidhu, P. K., Howes, N.K., Aung, T., Zwer, P.K., Davies, P.A. 2006. Factors affecting oat haploid production following oat × maize hybridization. Plant Breeding 125:243–247.CrossRefGoogle Scholar
  23. Suenaga, K. 1994. Doubled haploid system using the inter-generic crosses between wheat (Triticum aestivum L.) and maize (Zea mays L.). Bull. Natt. Inst. Agrobiol. Resour. 9:83–139.Google Scholar
  24. Verma, V., Bains, N.S., Mangat, G.S., Nanda, G.S., Gosal, S.S., Singh, K. 1999. Maize genotypes show striking differences for induction and regeneration of haploid wheat embryos in the wheat × maize system. Crop Science 39:1722–1727.CrossRefGoogle Scholar
  25. Zenkteler, M., Nitzsche, W. 1984. Wide hybridization experiments in cereals. Theor. Appl. Genet. 68:311–315.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2008

Authors and Affiliations

  1. 1.Faculty of AgricultureUniversity of BelgradeBelgradeSerbia
  2. 2.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany

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