Polyclonal Antibodies to Grain Gliadins and Their Application in Wheat Quality Prediction

Abstract

Gliadins were extracted from three wheat varieties, viz. Nongda 99260037 (good quality), Henan 9023 (media quality) and Nongda 98123 (poor quality), and α-, β-, γ-, ω -gliadins were purified from Nongda 99260037 using preparative SDS-PAGE. The total gliadins and α-, β-, γ-, ω -gliadins were used as antigens to immunise BALB/C mice, and the corresponding polyclonal antibodies were prepared, designated as Anti-A, Anti-B, Anti-C, Anti-A α, Anti-A β, Anti-A γ and Anti-A ω, respectively. Binding of the polyclonal antibodies with 8 varieties, that varied in quality properties, showed correlations with some wheat quality parameters. Correlation coefficients between antibodies against total gliadins or γ -, and ω -gliadin of Nongda 99260037 and quality parameters were higher than other antibodies. Of seven polyclonal antibodies tested, three (Anti-A γ, Anti-A ω and Anti-A) displayed significant positive or negative associations between antibody binding and dough development time, strength, valorimenter value and stability time, but no significant correlations were observed with water absorption. These results suggest that polyclonal antibodies could be used for rapid prediction and screening of wheat quality parameters.

References

  1. Andrews, J.L., Blundell, M.J., Skerritt, J.H. 1993. A simple antibody-based test for dough strength. III. Further simplification and collaborative evaluation for wheat quality screening. Cereal Chem. 70:241–246.

    CAS  Google Scholar 

  2. Andrews, J.L., Skerritt, J.H. 1996. Wheat dough extensibility screening using a two-site enzyme-linked immunosorbent assay (ELISA) with antibodies to low molecular weight glutenin subunits. Cereal Chem. 73:650–657.

    CAS  Google Scholar 

  3. Belton, P.S. 1999. On the elasticity of wheat gluten. J. Cereal Sci., 29:103–107.

    CAS  Article  Google Scholar 

  4. Branlard, G., Dardevet, M. 1985. Diversity of grain proteins and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics. J. Cereal Sci. 3:329–343.

    CAS  Article  Google Scholar 

  5. Brett, G.M., Mills, E.N.C., Tatham, A.S., Fido, R.J., Shewry, P.R., Morgan, M.R.A. 1993. Immunochemical identification of LMW subunits of glutenin associated with bread-making quality of wheat flours. Theor. Appl. Genet. 86:442–448.

    CAS  Article  Google Scholar 

  6. Feeney, K.A., Tatham, A.S., Gilbert, S.M., Fido, R.J., Halford, N.G., Shewry, P.R. 2001. Synthesis, expression and characterization of peptides comprised of perfect repeat motifs based on a wheat seed storage protein. Biochem. Biophys. Acta 1546:346–355.

    CAS  PubMed  Google Scholar 

  7. Festenstein, G.N., Hay, F.C., Shewry, P.R. 1987. Immunological relationship of the prolamin storage protein of barley, wheat, rye and oat. Biochem. Biophys. Acta 912:317–383.

    Google Scholar 

  8. Fido, R.J., Bekest, F., Grast, P.W., Tatham, A.S. 1997. Effects of α-, β-, γ- and ω -gliadins on the dough mixing properties of wheat flour. J. Cereal Sci. 26:271–277.

    CAS  Article  Google Scholar 

  9. Flate, N.E.S., Uhlen, A.K. 2003. Association between allelic variation at the combined Gli-1, Gli-3 loci and protein quality in common wheat (Triticum aestivum L.). J. Cereal Sci. 37:129–137.

    Article  Google Scholar 

  10. Fritschy, F., Windemann, H., Baumgartner, E. 1985. Quantitative of wheat gliadins in foods by enzyme linked immunosorbent assay. Z. Lebensm. Uniters Forsch., 181:379–385.

    CAS  Article  Google Scholar 

  11. Hill, A.S., Giersch, T.M., Loh, C.S., Skerritt, J.H. 1999. Immunoassay for wheat processing quality: Utilization of a sandwich assay incorporating an immobilized single-chain fragment. J. Agric. Food Chem. 47:4484–4490.

    CAS  Article  Google Scholar 

  12. Khatkar, B.S., Fidot, R.J., Tatham, A.S., Schofield, J.D. 2002. Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality. J. Cereal Sci. 3:299–306.

    Article  Google Scholar 

  13. MacRitchie, F. 1984. Baking quality of wheat flours. Adv. Food Res. 29:201–277.

    CAS  Article  Google Scholar 

  14. MacRitchie, F. 1987. Evaluation of contributions from wheat protein fractions to dough mixing and bread-making. J. Cereal Sci. 6:259–268.

    CAS  Article  Google Scholar 

  15. MacRitchie, F., DuCros, D.L., Wrigley, C.W. 1990. Flour polypeptides related to wheat quality. Adv. Cereal Sci. Tech. 10:79–145.

    CAS  Google Scholar 

  16. Martinez, Ma del C., Ruiz, M., Carrillo, J.M. 2005. Effects of different prolamin alleles on durum wheat quality properties. J. Cereal Sci. 41:123–131.

    CAS  Article  Google Scholar 

  17. Metakovsky, E.V., Annicchiarico, P., Boggini, G., Pogna, N.E. 1997a. Relationships between gliadin alleles and dough strength in Italian common wheats. J. Cereal Sci. 25:229–236.

    CAS  Article  Google Scholar 

  18. Metakovsky, E.V., Felix, I., Branlard, G. 1997b. Association between dough quality (W value) and certain gliadin alleles in French common wheat cultivars. J. Cereal Sci. 26:371–373.

    CAS  Article  Google Scholar 

  19. Payne, P.I., Seekings, J.A., Worland, A.J., Jarvis, M.G., Holt, L.M. 1987. Allelic variation of glutenin subunits and gliadins and its effect on bread-making quality in wheat: analysis of F5 progeny from Chinese Spring × Ch. Spring (Hope 1A). J. Cereal Sci. 6:103–118.

    Article  Google Scholar 

  20. Pogna, N.E., Boggini, G., Corbellini, M., Cattaneo, M., Peruffo, A.D.B. 1982. Association between gliadin electrophoretic bands and quality in common wheat. Can. J. Plant Sci. 4:913–918.

    Article  Google Scholar 

  21. Prabhasankar, P. 2002. Electrophoretic and immunochemical characteristics of wheat protein fractions and their relationship to chapati-making quality. Food Chem. 78:81–87.

    CAS  Article  Google Scholar 

  22. Skerritt, J.H. 1991a. A simple antibody-based test for dough strength. I. Development of the method and choice of antibodies. Cereal Chem. 68:467–474.

    Google Scholar 

  23. Skerritt, J.H. 1991b. Asimple antibody-based test for dough strength. II. Genotype and environmental effects. Cereal Chem. 68:475–481.

    Google Scholar 

  24. Song, X.Y., Cai, Q.F., Gao, W.S., Liu, G.T., Huang, T.C. 1998. Immunochemical assay for quality and immunological homology of storage proteins in different closely related genera and species of wheat. Acta Bot. Sin. 40:527–533.

    CAS  Google Scholar 

  25. Stenram, U., Heneen, W.K., Skerritt, J.H. 1991. Immunocytochemical localization of wheat storage protein in endosperm cells 30 days after anthesis. J. Exp. Bot. 42:1347–1355.

    CAS  Article  Google Scholar 

  26. Van Lonkhuijsen, H.J., Hamer, R.J., Schrender, C. 1992. Influence of specific gliadins on the bread making quality of wheat. Cereal Chem. 69:174–177.

    Google Scholar 

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Correspondence to X. Y. Song or Y. M. Yan.

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Li, Q.Y., Ji, K.M., Song, X.Y. et al. Polyclonal Antibodies to Grain Gliadins and Their Application in Wheat Quality Prediction. CEREAL RESEARCH COMMUNICATIONS 36, 117–124 (2008). https://doi.org/10.1556/CRC.36.2008.1.12

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Keywords

  • polyclonal antibodies
  • ELISA
  • gliadins
  • wheat quality