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Cereal Research Communications

, Volume 35, Issue 4, pp 1551–1562 | Cite as

Barley (Hordeum vulgare L.) Marker Linkage Map: A Case Study of Various Marker Types and of Mapping Population Structure

  • I. KarsaiEmail author
  • P. Szűcs
  • K. Mészáros
  • K. Puskás
  • Z. Bedő
  • O. Veisz
Open Access
Article

Abstract

A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.

Keywords

linkage map molecular markers barley Hordeum vulgare L. 

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Copyright information

© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • I. Karsai
    • 1
    Email author
  • P. Szűcs
    • 1
  • K. Mészáros
    • 1
  • K. Puskás
    • 1
  • Z. Bedő
    • 1
  • O. Veisz
    • 1
  1. 1.Agricultural Research Institute of the Hungarian Academy of SciencesMartonvásárHungary

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