High molecular weight glutenin and 1BL.1RS translocation employing the standard sodium dodecyl sulphate (SDS-PAGE) and acid (A-PAGE) polyacrylamide gel electrophoresis methods were classified in 43 Slovak wheat cultivars registered between 1976 and 2006. Total number of 9 alleles was detected at all Glu-1 loci. The most frequent HMW-GS alleles were “Null” for Glu-1A, 7+9 for Glu-1B and 5+10 for Glu-1D, respectively. At the same time these alleles also constituted the most frequent HMW-GS genotype and phenotype-0, 7+9, 5+10. Such HMW-GS combination was found in 48.8% of all genotypes analyzed in Triticum aestivum L. ssp. aestivum. Eleven different HMW-GS genotype-phenotype combinations were found, occurring at various frequencies.
Bradová, J., Šašek, A. 2005. Diversity of gliadins and HMW glutenïn subunits in Czech registered wheat varieties. Czech J. Genet. Plant Breed. 41 (Special Issue):160–163.
Buonocore, F., Hickman, D.R., Caporale, C., Porceddu, E., Lafiandra, D., Tatham, A.S., Shewry, P.R. 1996. Characterization of a novel high Mr glutenin subunit encoded by chromosome 1D of bread wheat. J. Cereal Sci. 23:55–60.
Černý, J., Šašek, A., Velj, P., Hanišová, A. 1995. Common wheat (Triticum aestivum L.) marking by determination of approximate dependence of frequency of allelic gliadin genes quality grade of agronomic character. Sci. Agric. Bohemoslovaca 26:245–258.
Cooke, R.J. 1995. Gel electrophoresis for the identification of plant varieties. J. Chromatography A 698:281–299.
Cooke, R.J. 1998. Seed storage protein diversity in wheat varieties. Plant Varieties and Seeds 11:159–167.
Draper, S.R. 1987. ISTA variety committee. Report of the working group for biochemical tests for cultivar identification 1983–1986. Seed Sci. Technol. 15:431–434.
Gálová, Z., Smolková, H., Michalík, I., Gregová, E. 1998. Predikcia pekárskej kvality zrna pšenice podl’a elektroforetického spektra HMW glutenínových subjednotiek. Rostlinná výroba 44:111–116.
Gröger, S., Oberforster, M., Werteker, M., Grausgruber, H., Lelley, T. 1997. HMW glutenin subunit composition and bread making quality of Austrian grown wheats. Cereal Res. Comm. 25:955–962.
Ivanov, P., Todorov, I., Stoeva, I., Ivanova, I. 1998. Storage proteins characterization of a group of new Bulgarian high breadmaking quality wheat lines. Cereal Res. Comm. 26:447–454.
Johansson E., Henrikson, P., Svensson, G., Heneeen, W.K. 1993. Detection, chromosomal location and evaluation of the functional value of a novel high Mr glutenin subunit found in Swedish wheats. J. Cereal Sci. 17:237–245.
Johansson, E., Svensson, G., Heneeen, W.K. 1995. Composition of high-molecular-weight glutenin subunits in Swedish wheats. Acta Agric. Scand. Sect. B, Soil and Plant Sci. 45:112–117.
Juhász, A., Larroque, O.R., Tamás, L., Hsam, S.L., Zeller, F.J., Békés, F., Bedő, Z. 2003. Bánkúti 1201 — an old Hungarian wheat variety with special storage protein composition. Theor. Appl. Genet. 107:697–704.
Kárpáti, M., Brieber, K., Kuroczi, L.G., Czirák, L., Lászity, R. 1995. Relationship between high molecular weight subunits of glutenin and breadmaking quality of Hungarian grown wheats. Acta Alimentaria 24:47–54.
Kolster, P., Krechting, K.F., van Gelder, W.M.J. 1993. Variation in type and relative amounts of the high molecular weight glutenin subunits in Dutch wheat varieties. J. Sci. Food Agric. 61:167–174.
Kolster, P., Vereijken, J.M. 1993. Evaluating HMW glutenin subunits to improve bread-making quality of wheat. Cereal Food World 2:76–83.
Lookhart, G.L., Hagman, K., Kasarda, D.D. 1993. High-molecular-weight glutenin subunits of the most commonly grown wheat cultivars in the U. S. in 1984. Plant Breed. 110:48–62.
Lukow, O.M., Payne, P.I., Tkachuk, P. 1989. The HMW glutenin subunit composition of Canadian wheat cultivars and their association with bread-making quality. J. Sci. Food. Agric. 46:451–460.
Morgunov, A.I., Rogers, W.J., Sayers, E.J., Metakovsky, E.V. 1990. The high-molecular weight glutenin subunit composition of Soviet wheat varieties. Euphytica 51:41–52.
Payne, P.I., Corfield, K.G., Blackmann, J.A. 1979. Identification of a high-molecular weight subunit of glutenin whose presence correlates with breadmaking quality in wheats of related pedigree. Theor. Appl. Genet. 55:153–159.
Payne, P.I., Lawrence, G.J. 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1, which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.
Rodriguez-Quijano, M., Vazquez, J.F., Carillo, J.M. 1990. Variation of high molecular weight glutenin subunits in Spanish landraces of Triticum aestivum ssp. vulgare and ssp. spelta. J. Genet. Breed. 44:121–126.
Sontag-Strohm, T. 1997. Gliadin and glutenin subunit alleles on group 1 chromosome in Finnish spring wheats. Acta Agric. Scand. Sect. B, Soil and Plant Sci. 47:98–105.
Uhlen, A.K. 1990. The composition of high molecular weight glutenin subunits in Norwegian wheats and their relation to bread-making quality. Norw. J. Agric. Sci. 4:1–17.
Wrigley, C.W. 1992. Identification of Cereal Varieties by Gel Electrophoresis of the Grain Proteins. Heidelberg, Springer-Verlag, pp. 17–41.
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Gregová, E., Mihálik, D., Šliková, S. et al. Allelic Variation of HMW Glutenin Subunits and 1BL.1RS Translocation in Slovak Common Wheats. CEREAL RESEARCH COMMUNICATIONS 35, 1675–1683 (2007). https://doi.org/10.1556/CRC.35.2007.4.14
- genetic diversity