Comparison of Hungarian and Bulgarian Isolates of Maize Dwarf Mosaic Virus

Abstract

Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen. Maize dwarf mosaic virus (MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.

References

  1. Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L., Zurcher, E.J. (eds) 1996 onwards. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20 th August 1996. URL

  2. Frenkel, M.J., Jilka, J.M., McKern, N.M., Strike, P.M., Clark Jr., J.M., Shukla, D.D., Ward, C.W. 1991. Unexpected sequence diversity in the amino-terminal ends of the coat proteins of strains of sugarcane mosaic virus. Journal of General Virology 72:237–242.

    CAS  Article  Google Scholar 

  3. Janson, B.F., Ellett, C.W. 1963. A new corn disease in Ohio. Plant Dis. Rep. 47:1107–1108.

    Google Scholar 

  4. Jarjees, M.M., Uyemoto, J.K. 1984. Serological relatedness of strains of maize dwarf mosaic and sugarcane mosaic viruses as determined by microprecipitin and enzyme-linked immunosorbent assays. Ann. Appl. Biol. 104:497–501.

    Article  Google Scholar 

  5. Kong, P., Steinbiss, H.-H. 1998. Complete nucleotide sequence and analysis of the putative polyprotein of maize dwarf mosaic virus genomic RNA (Bulgarian isolate). Archives of Virology 143:1791–1799.

    CAS  Article  Google Scholar 

  6. Kovachevsky, I., Markov, M., Yankulova, M., Trifonov, D., Stoyanov, D., Kacharmazov, V. 1995. Virus and Virus-like Diseases of Crop Plants. PSSA, Sofia, pp. 143–148. (in Bulgarian)

    Google Scholar 

  7. Markov, M. 1972. Studies on maize mosaic in Bulgaria. I. Identification of the virus. Plant Science 9:171–179. (in Bulgarian with English and Russian summary)

    Google Scholar 

  8. Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. II. Establishing the host plants of the virus. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 55–67. (in Bulgarian with English and Russian summary)

    Google Scholar 

  9. Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. III. Virus purification. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 139–145. (in Bulgarian with English and Russian summary)

    Google Scholar 

  10. Pirone, T.P., Blanc, S. 1996. Helper-dependent vector transmission of plant viruses. Ann. Rev. Phytopathology 34:227–247.

    CAS  Article  Google Scholar 

  11. Rosenkranz, E. 1987. New hosts and taxonomic analysis of the Mississippi native species tested for reaction to maize dwarf mosaic and sugarcane mosaic viruses. Phytopathology 77:598–607.

    Article  Google Scholar 

  12. Seifers, D.L., Salomon, R., Marie-Jeanne, V., Alliot, B., Signoret, P., Haber, S., Loboda, A., Ens, W., She, Y.-M., Standing, K.G. 2000. Characterization of a novel potyvirus isolated from maize in Israel. Phytopathology 90:505–513.

    CAS  Article  Google Scholar 

  13. Shepherd, R.J. 1965. Properties of a mosaic virus of corn and Johnson grass and its relation to the sugarcane mosaic virus. Phytopathology 55:1250–1256.

    Google Scholar 

  14. Shukla, D.D., Tosic, M., Jilka, J., Ford, R.E., Toler, R.W., Langham, M.A.C. 1989. Taxonomy of potyviruses infecting maize, sorghum, and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79:223–229.

    CAS  Article  Google Scholar 

  15. Shukla, D.D., Frenkel, M.J., McKern, M.N., Ward, C.W., Jilka, J., Tosic, M., Ford, R.E. 1992. Present status of sugarcane mosaic subgroup of potyviruses. Archives of Virology Suppl. 5:363–373.

    CAS  Article  Google Scholar 

  16. Shukla, D.D., Ward, C.W., Brunt, A.A. 1994. The Potyviridae. CAB International, Wellingford, UK.

    Google Scholar 

  17. Snazelle, T.E., Bancroft, J.B., Ullstrup, A.J. 1971. Purification and serology of maize dwarf mosaic and sugarcane mosaic viruses. Phytopathology 61:1059–1063.

    Article  Google Scholar 

  18. Szirmai, J., Paizs, L-né 1963. Maize mosaic or stripe. Növénytermelés 12:43–50. (in Hungarian, with English summary)

    Google Scholar 

  19. Tóbiás, I., Palkovics, L. 2004. An unusual feature at the N-terminal end of the coat protein of Maize dwarf mosaic virus isolated in Hungary. Journal of Phytopathology 152:445–447.

    Article  Google Scholar 

  20. White, J.L., Kaper, J.M. 1989. A simple method for detection of viral satellite RNAs in small tissue samples. Journal of Virological Methods 23:83–94.

    CAS  Article  Google Scholar 

  21. Williams, L.E., Alexander, L.J. 1965. Maize dwarf mosaic virus, a new corn disease. Phytopathology 58:802–804.

    Google Scholar 

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Correspondence to I. Tóbiás.

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Tóbiás, I., Bakardjieva, N. & Palkovics, L. Comparison of Hungarian and Bulgarian Isolates of Maize Dwarf Mosaic Virus. CEREAL RESEARCH COMMUNICATIONS 35, 1643–1651 (2007). https://doi.org/10.1556/CRC.35.2007.4.11

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Keywords

  • Maize dwarf mosaic virus
  • corn
  • coat protein
  • sequence similarity
  • SCMV subgroup