Expression of the 1D×5 High Molecular Weight Glutenin Subunit Protein in Transgenic Rice
We generated and analysed transgenic rice lines synthesizing substantial amounts of high-molecular-weight glutenin subunit (HMW-GS) from wheat. Particle bombardment has been used to transform rice cultivars (Orysa sativa L.) with a cassette carrying the gene of 1D×5 HMW glutenin subunit. Twelve independent lines were recovered and PCR results on genomic DNS confirmed the integration of the transgene into it. Five lines set seeds. Seeds were analysed by SDS-PAGE and Western blot and we proved the presence of foreign protein in the starchy endosperm. The amount of 1D×5 HMW-GS in rice endosperm represents 0.75–3.18% of the alcohol soluble proteins. These results are the first example of significantly changing storage protein composition of rice exploiting the method of gene technology. This alteration may have considerable effect on the functional properties, including strength and stability of the dough made of transgenic rice flour.
Keywordswheat storage proteins HMW glutenin expression functional properties rice endosperm
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