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Cereal Research Communications

, Volume 35, Issue 4, pp 1543–1549 | Cite as

Expression of the 1D×5 High Molecular Weight Glutenin Subunit Protein in Transgenic Rice

  • M. Oszvald
  • B. Jenes
  • S. Tömösközi
  • F. Békés
  • L. TamásEmail author
Open Access
Article

Abstract

We generated and analysed transgenic rice lines synthesizing substantial amounts of high-molecular-weight glutenin subunit (HMW-GS) from wheat. Particle bombardment has been used to transform rice cultivars (Orysa sativa L.) with a cassette carrying the gene of 1D×5 HMW glutenin subunit. Twelve independent lines were recovered and PCR results on genomic DNS confirmed the integration of the transgene into it. Five lines set seeds. Seeds were analysed by SDS-PAGE and Western blot and we proved the presence of foreign protein in the starchy endosperm. The amount of 1D×5 HMW-GS in rice endosperm represents 0.75–3.18% of the alcohol soluble proteins. These results are the first example of significantly changing storage protein composition of rice exploiting the method of gene technology. This alteration may have considerable effect on the functional properties, including strength and stability of the dough made of transgenic rice flour.

Keywords

wheat storage proteins HMW glutenin expression functional properties rice endosperm 

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Copyright information

© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. Oszvald
    • 1
    • 2
  • B. Jenes
    • 2
  • S. Tömösközi
    • 1
  • F. Békés
    • 3
  • L. Tamás
    • 4
    Email author
  1. 1.Department of Applied Biotechnology and Food ScienceBudapest University of Technology and EconomicsBudapestHungary
  2. 2.Plant Transformation GroupAgricultural Biotechnology CenterGödöllőHungary
  3. 3.CSIROPlant IndustryCanberraAustralia
  4. 4.Department of Plant Physiology and Molecular Plant BiologyEötvös Loránd UniversityBudapestHungary

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