Advertisement

Cereal Research Communications

, Volume 35, Issue 1, pp 15–22 | Cite as

The Effect of Liquid and Solid Medium on Production of Winter Triticale (× Triticosecale Wittm.) Anther-Derived Embryos and Plants

  • A. PonitkaEmail author
  • A. Ślusarkiewicz-Jarzina
Article

Abstract

The efficiency of anther culture induction in solid and liquid medium C17 was compared for nine triticale genotypes. For all genotypes androgenic embryos and green plants efficiency was higher on liquid medium. On liquid medium 22.2–135.9 and on solid medium 27.5–121.3 androgenic embryos/100 anthers were produced. The embryos were transferred to a regeneration medium 190-2 and also a higher number of green plants (ranging from 3.0 to 31.4/100 anthers) were obtained in liquid culture compared with the yield of plants (1.6–21.0) on solid medium.

Keywords

anther culture winter triticale liquid and solid medium androgenic plants 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arzani, A., Darvey, N.L. 2001. The effect of colchicine on triticale anther-derived plants: Microspore pre-treatment and haploid-plant treatment using a hydroponic recovery system. Euphytica 122:235–241.CrossRefGoogle Scholar
  2. Arzani, A., Darvey, N.L. 2002. Androgenetic response of heterozygous triticale populations using a greenhouse hydroponic system. Euphytica 127:53–60.CrossRefGoogle Scholar
  3. Charmet, G., Bernard, S. 1984. Diallel analysis of androgenetic plant production in hexaploid Triticale (× Triticosecale Wittmack). Theor. Appl. Genet. 69:55–61.CrossRefGoogle Scholar
  4. Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., Bi, F.Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18:659–668.Google Scholar
  5. Chu, C.C., Hill, R.D. 1988. An improved anther culture method for obtaining higher frequency of pollen embryoids in Triticum aestivum L. Plant Sci. 55:175–181.CrossRefGoogle Scholar
  6. Eudes, F., Amundsen, E. 2005. Isolated microspore culture of Canadian 6x triticale cultivars. Plant Cell Tiss. Org. Cult. 82:233–241.CrossRefGoogle Scholar
  7. Guo, Y.D., Sewón, P., Pulli, S. 1999. Improved embryogenesis from anther culture and plant regeneration in timothy. Plant Cell Tiss. Org. Cult. 57:85–93.CrossRefGoogle Scholar
  8. Hassawi, D.S., Liang, G.H. 1990. Effect of cultivar, incubation temperature, and stage of microspore development on anther culture in wheat and triticale. Plant Breed. 105:332–336.CrossRefGoogle Scholar
  9. Henry, Y., De Buyser, J. 1981. Float culture of wheat anthers. Theor. Appl. Genet. 60:77–79.CrossRefGoogle Scholar
  10. Immonen, S., Robinson, J. 2000. Stress treatments and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci. 150:77–84.CrossRefGoogle Scholar
  11. Inagaki, M.N., Pfeiffer, W.H., Mergoum, M., Mujeeb-Kazi, A., Lukaszewski, A.J. 1997. Effects of D-genome chromosomes on cross ability of hexaploid triticale (× Triticosecale Wittmack) with maize. Plant Breed. 116:387–389.CrossRefGoogle Scholar
  12. Inagaki, M.N., Hash, C.T. 1998. Production of haploids in bread wheat, durum wheat and hexaploid triticale crossed with pearl millet. Plant Breed. 117:485–487.CrossRefGoogle Scholar
  13. Karsai, I., Bedő, Z., Hayes, P.M. 1994. Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat. Plant Cell Tiss. Org. Cult. 39:49–53.CrossRefGoogle Scholar
  14. Kiviharju, E.M., Tauriainen, A.A. 1999. 2,4-Dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses: plant regeneration from A. sativa L. Plant Cell Rep. 18:582–588.CrossRefGoogle Scholar
  15. Ma, R., Guo, Y-D., Pulli, S. 2004. Comparison of anther and microspore culture in the embryogenesis and regeneration of rye (Secale cereale). Plant Cell Tiss. Org. Cult. 76:147–157.CrossRefGoogle Scholar
  16. Monostori, T., Puolimatka, M., Pauk, J. 1998. Triticale (× Triticosecale Wittmack) in vitro androgenesis in isolated microspore culture. Növénytermelés 47:371–382.Google Scholar
  17. Oleszczuk, S., Sowa, S., Zimny, J. 2004. Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (× Triticosecale Wittmack) cv. Bogo. Plant Cell Rep. 22:885–893.PubMedGoogle Scholar
  18. Pauk, J., Puolimatka, M., Tóth, K.L., Monostori, T. 2000. In vitro androgenesis of triticale in isolated microspore culture. Plant Cell. Tiss. Org. Cult. 61:221–229.CrossRefGoogle Scholar
  19. Ponitka, A., Ślusarkiewicz-Jarzina, A., Wędzony, M., Marcińska, I., Woźna, J. 1999. The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (× Triticosecale Wittm.). J. Appl. Genet. 40:165–174.Google Scholar
  20. Pratap, A., Sathi, G.S., Chaudhary, H.K. 2005. Relative efficiency of different Gramineae genera for haploid induction in triticale and triticale × wheat hybrids through the chromosome elimination technique. Plant Breed. 124:147–153.CrossRefGoogle Scholar
  21. Puolimatka, M., Pauk, J. 2000. Effect of induction duration and medium composition on plant regeneration in wheat (Triticum aestivum L.) anther culture. J. Plant Physiol. 156:197–203.CrossRefGoogle Scholar
  22. Sharma, C.C., Wang, W.C., Sapra, V.T. 1982. Effect of genotype, media and temperature pretreatment on callus initiation in triticale, wheat and rye anther cultures. Cereal Res. Comm. 10:143–150.Google Scholar
  23. Sozinov, A., Lukyanyuk, S., Ignatova, S. 1981. Anther cultivation and induction of haploid plants in triticale. Z. Pflanzenzüchtg. 86:272–285.Google Scholar
  24. Ślusarkiewicz-Jarzina, A., Ponitka, A. 1997. Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale. J. Appl. Genet. 38:253–258.Google Scholar
  25. Ślusarkiewicz-Jarzina, A., Ponitka, A. 2003. Efficient production of spontaneous and induced doubled haploid triticale plants derived from anther culture. Cereal Res. Comm. 31:289–296.Google Scholar
  26. Wang, P., Chen, Y. 1983. Preliminary study on production of height of pollen H2 generation in winter wheat grown in the field. Acta Agron. Sin. 9:283–284.Google Scholar
  27. Wang, X., Hu, H. 1984. The effect of potato II medium for triticale anther culture. Plant Sci. Lett. 36:237–239.CrossRefGoogle Scholar
  28. Warzecha, R., Sowa, S., Salak-Warzecha, K., Oleszczuk, S., Śliwińska, E., Zimny, J. 2005. Doubled haploids in production of male sterility maintaining triticale (Triticosecale Wittmack) lines. Acta Physiol. Plant. 27:245–250.CrossRefGoogle Scholar
  29. Wędzony, M., Marcińska, I., Ponitka, A., Ślusarkiewicz-Jarzina, A., Woźna, J. 1998. Production of doubled haploids in triticale (× Triticosecale Wittm.) by means of crosses with maize (Zea mays L.) using picloram and dicamba. Plant Breed. 117:211–215.CrossRefGoogle Scholar
  30. Zhou, H., Zheng, Y., Konzak, C.F. 1991. Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10:63–66.CrossRefGoogle Scholar
  31. Zhuang, J.J., Xu, J. 1983. Increasing differentiation frequencies in wheat pollen callus. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement. Science Press, Beijing, p. 431.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

Authors and Affiliations

  1. 1.Institute of Plant GeneticsPolish Academy of SciencesPoznańPoland

Personalised recommendations