Cereal Research Communications

, Volume 35, Issue 1, pp 119–128 | Cite as

Protein Concentration and Composition in Wheat Flour Mill Streams

  • A. OkrajkováEmail author
  • M.-L. Prieto-Linde
  • Z. Muchová
  • E. Johansson
Open Access


Protein concentration and composition varies in different part of the wheat grain and also in different mill streams. This investigation aimed at determining protein concentration, composition and disulfide crosslinkings in different wheat flour mill streams. Flour mill streams were obtained by milling grains of two Slovakian bread wheat cultivars in a Brabender Quadromat Senior test mill. Protein concentration, amount of protein groups, amount and size distribution of polymeric and monomeric proteins, free thiol groups and disulfide crosslinkings were measured. The results showed that the break flour contained high amounts of gliadins and glutenin subunits, SDS-extractable large polymeric proteins and disulfide crosslinkings. The shorts contained high amounts of SDS-extractable and SDS-unextractable smaller monomeric proteins, SDS-unextractable larger monomeric proteins and free thiol groups. The bran contained high protein concentration, high amounts of high-molecular-weight glutenin subunits, SDS-extractable and SDS-unextractable smaller monomeric proteins and SDS-unextractable larger monomeric proteins.


disulfide crosslinkings protein concentration protein composition wheat mill streams 


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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Okrajková
    • 1
    Email author
  • M.-L. Prieto-Linde
    • 2
  • Z. Muchová
    • 1
  • E. Johansson
    • 2
  1. 1.Department of Storing and Processing Plant ProductsSlovak University of AgriculturalNitraSlovakia
  2. 2.Department of Crop ScienceThe Swedish University of Agricultural SciencesAlnarpSweden

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