Advertisement

Cereal Research Communications

, Volume 35, Issue 1, pp 1–10 | Cite as

Fine Location of the S-5 Locus Responsible for Wide Compatibility in Rice Using SSR Markers

  • X. J. Zhang
  • Y. Z. Chen
  • Y. P. Wei
  • W. L. Lu
  • H. H. Liao
  • Y. F. Liu
  • X. Q. Yang
  • X. Y. Li
  • L. Yang
  • L. S. Li
  • R. B. LiEmail author
Article

Abstract

Partial abortion of gametes possessing S-5j in S-5i/S-5j genotype at locus S-5 is responsible for hybrid sterility between indica and japonica subspecies in rice (Oryza sativa L.), while a single wide compatibility (WC) allele S-5n can restore normal hybrid fertility between the two groups. In this study, Pei’ai 64S, one of the most popular WC line widely used for subspecific hybrid rice breeding program in South China was studied for location of its S-5 locus. Twenty SSR (Simple Sequence Repeat) markers derived from Cornell SSR linkage map and 9 developed using sequences from GenBank database were employed to perform bulked segregant analysis of the mapping population derived from a three-way cross (Pei’ai 64S/T8//Akihikari) to tag fine location of the hybrid sterility locus, S-5. This S-5 locus was mapped on chromosome 6 approximately 0.2 cM from GXR6 and RM276 SSR markers. This tight linkage of the markers and the S-5 locus would be very useful for efficient marker-assisted selection for WC varieties and for map-based cloning of the gene.

Keywords

hybrid sterility Oryza sativa SSR marker wide compatibility 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allard, R.W. 1956. Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278.CrossRefGoogle Scholar
  2. Bai, D.L., Luo, X.H. 1995. The combination and exploitation of new two-line rice hybrid, Liangyou Peite. Hunan Agr. Sci. 6:6–8.Google Scholar
  3. Ikehashi, H., Araki, H. 1984. Variety screening of compatibility types revealed in F1 fertility of distant cross in rice. Jpn. J. Breed. 34:304–313.CrossRefGoogle Scholar
  4. Ikehashi, H., Araki, H. 1986. Genetics of F1 sterility in remote crosses of rice. In: Khush, G.S. (ed.), Rice Genetics. IRRI, P.O. Box 933, Manila, Philippines, pp. 119–130.Google Scholar
  5. Ikehashi, H., Araki, H., Yanagihara, S. 1991. Screening and analysis of wide compatibility loci in wide crosses of rice. In: Khush, G.S. (ed.), Rice Genetics II, 33–43. IRRI, P.O. Box 933, Manila, Philippines.Google Scholar
  6. Ikehashi, H., Wan, J. 1998. The wide compatibility system: current knowledge of its genetics and use for enhanced yield heterosis. In: Vermani, S.S., Siddiq, E.A., Muralidharan, K. (eds), Advances in Hybrid Rice Technology. IRRI, Manila, Philippines, pp. 67–77.Google Scholar
  7. Li, H., Tang, L., Zou, J. 1991. Marker-based analysis of wide compatibility in rice (in Chinese). Hybrid Rice 4:22–24.Google Scholar
  8. Liu, A., Zhang, Q., Li, H. 1992. Location of a gene for wide-compatibility in the RFLP linkage map. Rice Genet. Newsl. 9:134–136.Google Scholar
  9. McCouch, S.R., Teytelman, L., Xu, Y., Lobos, K.B., Clare, K., Walton, M., Fu, B., Maghirang, R., Li, Z., Xing, Y., Zhang, Q., Kono, I., Yano, M., Fjellstrom, R., DeClerck, G., Schneider, D., Cartinhour, S., Ware, D., Stein, L. 2002a. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Research 9:199–207.CrossRefGoogle Scholar
  10. McCouch, S.R., Teytelman, L., Xu, Y., Lobos, K.B., Clare, K., Walton, M., Fu, B., Maghirang, R., Li, Z., Xing, Y., Zhang, Q., Kono, I., Yano, M., Fjellstrom, R., DeClerck, G., Schneider, D., Cartinhour, S., Ware, D., Stein, L. 2002b. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.) (Supplement). DNA Research 9 Suppl.: 257–279.CrossRefGoogle Scholar
  11. Michelmore, R.W., Paran, I., Kesseli, R.V. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA 88:9828–9832.CrossRefGoogle Scholar
  12. Murray, M.G., Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321–4325.CrossRefGoogle Scholar
  13. Panaud, O., Chen, X., McCouch, S.R. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol. Gen. Genet. 252:597–607.PubMedGoogle Scholar
  14. Wan, J., Yanagihara, S., Kato, H., Ikehashi, H. 1993. Multiple alleles at a new locus causing hybrid sterility between a Korean Indica variety and a Javanica variety in rice (Oryza sativa L.). Jpn. J. Breed. 43:507–516.CrossRefGoogle Scholar
  15. Wan, J., Ikehashi, H. 1995a. Identification of a new locus S-16 causing hybrid sterility in native rice varieties (Oryza sativa L.) from Tai-hu lake region and Yunnan Province, China. Breed. Sci. 45,:461–470.Google Scholar
  16. Wan, J., Ikehashi, H. 1995b. A new locus hybrid sterility in remote crosses of cultivated rice (Oryza sativa L.) 6. Breed. Sci. 45, Suppl. 2: 191.Google Scholar
  17. Wan, J., Yanagihara, S., Kato, H., Ikehashi, H. 1996. Two new loci for hybrid sterility in rice (Oryza sativa L.). Theor. Appl. Genet. 92:83–90.CrossRefGoogle Scholar
  18. Wang, Z.Y., Tanksley, S.T. 1989. Restriction fragment length polymorphism in Oryza sativa L. Genome 32:1113–1118.CrossRefGoogle Scholar
  19. Wu, K.S., Tanksley, S.D. 1993. Abundance, polymorphism and genetic mapping of microsatellite in rice. Mol. Gen. Genet. 241:225–235.CrossRefGoogle Scholar
  20. Yanagihara, S., Kato, H., Ikehashi, H. 1992. A new locus for multiple alleles causing hybrid sterility between an Aus variety and Javanica varieties in rice (Oryza sativa L.) Japan. J. Breed. 42:793–801.CrossRefGoogle Scholar
  21. Yanagihara, S., McCouch, S. R., Ishikawa, K., Ogi, Y., Maruyama, K., Ikehashi, H. 1995. Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in indica/japonica hybrids rice (O. sativa L.). Theor. Appl. Genet. 90:182–188.CrossRefGoogle Scholar
  22. Yuan, L.P. 1992. The strategy of the development of hybrid rice breeding. In: Yuan, L.P. (ed.), Current Status of Two Line Hybrid Rice Research. Agricultural Publishing LTD, Beijing, pp. 1–5.Google Scholar
  23. Zheng, K., Shen, P., Qing, H., Wang, J. 1992. Tagging genes for wide compatibility in rice via linkage to SSR markers. Chinese J. Rice Sci. 6:145–150.Google Scholar
  24. Zou, J., Nie, Y., Pan, Q., Fu, C. 1992. The tentative utilization of wide compatibility strain 02428 in indica/Japonica hybrid rice. In: Yuan, L.P. (ed.), Current Status of Two Line Hybrid Rice Research. Agricultural Publishing LTD, Beijing, pp. 333–339.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

Authors and Affiliations

  • X. J. Zhang
    • 1
    • 5
  • Y. Z. Chen
    • 1
    • 2
    • 5
  • Y. P. Wei
    • 3
  • W. L. Lu
    • 1
    • 5
  • H. H. Liao
    • 1
    • 5
  • Y. F. Liu
    • 1
    • 4
    • 5
  • X. Q. Yang
    • 4
  • X. Y. Li
    • 4
  • L. Yang
    • 1
    • 5
  • L. S. Li
    • 1
    • 2
  • R. B. Li
    • 1
    • 5
    Email author
  1. 1.Guangxi Crop Genetic Improvement and Biotechnology LabGuangxi Academy of Agricultural SciencesNanningChina
  2. 2.Agricultural CollegeGuangxi UniversityNanningChina
  3. 3.Agro-information Research InstituteGuangxi Academy of Agricultural SciencesNanningChina
  4. 4.Rice Research InstituteGuangxi Academy of Agricultural SciencesNanningChina
  5. 5.Guangxi Key Laboratory of Subtropical Bioresource Conversation and UtilizationGuangxi UniversityNanningChina

Personalised recommendations