Cereal Research Communications

, Volume 34, Issue 4, pp 1247–1254 | Cite as

Characterization of Hordeum vulgare accessions of Pakistan by Hordein SDS-PAGE

  • Rehan Naeem
  • Ibrar Ahmed
  • Rehana Asghar
  • Bushra MirzaEmail author


One hundred and sixty Pakistani Hordeum vulgare ssp. vulgare accessions were analyzed for genetic diversity on the basis of hordein, seed storage proteins. In total we have analyzed 7 Hor-1, 12 Hor-2 and 5 Hor-3 alleles for three hordein loci in barley accessions on SDS-PAGE. Out of 24 polymorphic alleles, three rare alleles (Hor 3.1, Hor 2.1, Hor 1.1) were detected. Abundant genetic variability was observed in Pakistani barley accessions for hordein loci. Genetic similarities calculated for all pair wise comparisons of H. vulgare accessions were used to form 83 banding patterns that represent the core collections of Pakistan, which included 50 unique patterns. Multivariate analysis conducted to generate similarity matrix using Jaccard’s coefficient (Jaccard, 1908) to estimate relatedness and divergence among 160 accessions ranged from 0.11 to 1.00, representing high level of genetic variability. Clustering was carried out to determine genetic diversity among the core collections that clustered them into three major clusters. In this study genetic diversity for cultivated barley belonging to different regions of Pakistan were in the order of Punjab> Balochistan> Northern Area> Sindh> A.J.K.> N.W.F.P.


Barley germplasm hordein genetic diversity allele frequency SDS-PAGE 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Atanassov, P., Borries, C., Zaharieva, M., Monneveux, P. 2001. Hordein polymorphism and variation of agromorphological traits in a collection of naked barley. Genet. Resource. Crop Evol. 48: 353–360.CrossRefGoogle Scholar
  2. Bernardo, A., Luque, A., Cuadrado, A., Negro, A., Jouve, N., Soler, C. 1997. The assessment of genetic variation in Spanish primitive cultivars of barley by a combination of isozyme and hordeins. Genet. Resource. Crop Evol. 44(3): 217–226.CrossRefGoogle Scholar
  3. Bergal, P., Friedberg, L. 1940. Essai d’identification des orges cultivées en France. Annals Epiphyt. Phytogenetique 6: 2–4.Google Scholar
  4. Bothmer R. von. 1992. The wild species of Hordeum, relationships and potential use for improvement of cultivated barley. In: Barley: Genetics, Biochemistry, molecular biology and biotechnology, eds., P. R. Shewry, CAB International, Oxford, pp: 3–18.Google Scholar
  5. Cattivelli, L., Komjanc, M., Terzi, V., Odoardi, M. 1987. Identificazione delle varietà di orzo mediante elettroforesi delle ordeine. Sementi Elette 3: 3–8.Google Scholar
  6. Choumane, W., Achtar, S., Valkoun J., Weigand, F. 1998. Genetic variation in core and base barley collections from WANA as revealed by RAPDs. In: Triticaceae III, ed., A. A. Jaradat, Science Publishers Inc., New Hampshire, USA, pp: 159–163.Google Scholar
  7. Doll, H., Andersen, B. 1981. Preparation of barley storage protein, hordein, for analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 115: 61–66.CrossRefGoogle Scholar
  8. Echart-Almeida, C., Cavalli-Molina, S. 2000. Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties. Genet. Mol. Biol. 23(2): 425–433.CrossRefGoogle Scholar
  9. Erskine, W., Muehlbauer, F.J. 1991. Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. Theor. Appl. Genet. 83: 119–125.CrossRefGoogle Scholar
  10. Frankel, O. H., Brown, A.H.D. 1984. Plant genetic resources today: a critical appraisal. In: Crop genetic resources: conservation and evaluation, eds., J.H.W. Holden, J.T. Williams, G. Allen and U. Winchester, Massachusetts, pp. 249–268.Google Scholar
  11. Hintum, T.J.L. 1994. Comparison of marker systems and construction of a core collection in a pedigree of European spring barley. Theor. Appl. Genet. 89: 991–997.CrossRefGoogle Scholar
  12. Hoffman, D. L. Bregitzer, P. 1996. Identification of Reproducible PCR-RAPD Markers that Enable the Differentiation of Closely Related Six-Rowed Malting Barley (Hordeum vulgare L.) Cultivars. J. Am. Soc. Brew. Chem. 54(3): 172–176.Google Scholar
  13. Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vaudoise Sci. Nat. 44: 223–270.Google Scholar
  14. Laemmli, U. K. 1970. Cleavage of structural proteins assembly of the head of bacteriophage T4. Nature 22: 680–685.CrossRefGoogle Scholar
  15. Mackay, M.C. 1986. Utilizing wheat genetic resources in Australia. In: Proceedings of 5th Assembly Wheat Breed Soc., Australia, ed., R. McLean, Perth/Merredin, Australia, pp: 56–61.Google Scholar
  16. Marchylo, B.A., Laberge, D.E. 1981. Barley cultivar identification by electrophoretic analysis of hordein proteins. II. Catalogue of electrophoregram formulae for Canadian-grown barley cultivars. Can. J. Plant Sci. 60: 859–870.CrossRefGoogle Scholar
  17. Marchylo, B.A. 1987. Barley cultivar identification by SDS gradient PAGE analysis of hordein. Can. J. Plant Sci. 67: 927–944.CrossRefGoogle Scholar
  18. Nielsen, G., Johansen, H.B. 1986. Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isozyme loci of 47 reference varieties. Euphyt. 35: 717–728.CrossRefGoogle Scholar
  19. Radovic, D., Vapa, L. 1996. Hordein composition of Yugoslav barley cultivars. Cereal Res. Comm. 24(3): 331–337.Google Scholar
  20. Saghai-Maroof, M.A., Biyashev, R.M., Yang, G.P., Zhang, Q., Allard, R.W. 1994. Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics. Proc. Nat. Acad. Sci. 91: 5466–5470.CrossRefGoogle Scholar
  21. Shewry, P.R., Ellis, J.R.S., Pratt, H.M., Miflin, B.J. 1978a. A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties. J. Sci. Food Agric. 29: 433–441.CrossRefGoogle Scholar
  22. Shewry, P.R., Faulks, A.J., Pickering, R.A., Jones, I.T., Finch R.A., Miflin, B.J. 1980. The genetic analysis of barley storage proteins. Hered. 44: 383–389.CrossRefGoogle Scholar
  23. Thompson, J.A., Nelson R.L., Vodkin, L.O. 1998. Identification of diverse soybean germplasm using RAPD markers. Crop Sci. 38: 1348–1355.CrossRefGoogle Scholar
  24. Vapa, L., Radovic, D. 1998. Genetics and molecular biology of barley hordeins. Cereal Res. Comm. 26(1): 31–38.Google Scholar
  25. Virk, P.S., Newbury, H.J., Jackson, M.T., Ford-Lloyd, B.V. 1995. The identification of duplicate accessions within a rice germplasm collection using RAPD analysis. Theor. Appl. Genet. 90: 1049–1055.CrossRefGoogle Scholar
  26. Yin, O. Y., Ma, D.O., Ding, Y. 2003. Analysis of Genetic diversity of hordein in wild close relatives of barley from Tibet. Theor. Appl. Genet. 107: 837–842.CrossRefGoogle Scholar
  27. Zhang, Q., Sahgai-Maroof, M. A., Kleinhofs, A. 1993. Comparative diversity analysis of RFLPs and isozymes within and among populations of Hordeum vulgare ssp. spontaneum. Genetics 134: 901–911.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2006

Authors and Affiliations

  • Rehan Naeem
    • 1
  • Ibrar Ahmed
    • 1
  • Rehana Asghar
    • 1
    • 2
  • Bushra Mirza
    • 1
    Email author
  1. 1.Department of BiochemistryQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Department of BotanyUniversity of Arid AgricultureRawalpindiPakistan

Personalised recommendations