Cereal Research Communications

, Volume 34, Issue 2–3, pp 1029–1036 | Cite as

Anther culture response in heterozygous triticale (× Triticosecale Wittmack) populations

  • Magdalena GutEmail author
  • Andrzej Bichoński
  • Wojciech Mikulski
  • Hanna Bielerzewska


The subject of research was the anther culture response of 186 lines of winter crop triticale coming from two types of crossings: A×B and (A×B)×C. The number of embryoids and green regenerants obtained from particular lines were adopted as property indicators. The examined lines were characterised by a relatively good ability to create either single embryoids or conglomerated embryoids, but the number of green regenerants obtained later on from such structures was generally low. It was found that there were no significant differences between these objects both with regard to the ability to create embryoids and green regenerants in the case of anthers collected from hybrids from simple crossings, whilst there were significant differences (with p=0.05) in the case of hybrids originated from crossings of the type (A×B)×C. The coefficients of heritability of the ability to create embryoids was h 2 =0,682 for anthers coming from hybrids of the type: A×B and h 2 =0,371 for anthers collected from hybrids of the (A×B)×C type. However genetic conditioning of the ability to regenerate green plants was 0.699 and 0.522 respectively. It may thus be supposed that the ability to create embryoids passed onto the next generation would be better with the forms originating from crossing of the A×B type, and the heritability of the ability to create green plants would be similar with both types of hybrids. For both of the groups compared, also the coefficients of variability CV(p) and CV(g) were calculated as well as the coefficients of correlation between the number of embryoids and the number of green regenerants obtained from them at a later phase.

Key words

androgenesis heritability winter triticale 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arzani A., Darvey N.L., 2002. Androgenetic response of heterozygous triticale populations using a greenhouse hydroponic system. Euphytica 127: 53–60CrossRefGoogle Scholar
  2. Baker J., Bendelow V.M., Kaufmann M.L. 1968. Inheritance and interrelationship among yield and several quality traits in common wheat. Crop. Sci. 8: 725:728.Google Scholar
  3. Bullock W.P., Beamier P.S., Schaeffer G.W., Bottino P.S. 1982. Anther culture of wheat (Triticum aestivum L.) F 1’ s and their reciprocal crosses. Theor. Appl. Genet. 59:333–340Google Scholar
  4. Chu C.C., Wang C.C., Sun C.S., Hsu C., Yin K.C., Chu C.Y., Bi F.Y., 1975. Establishment of an effect medium for anther culture of rice through comparative experiment on the nitrogen sources. Sci. Sinica 18: 659–668.Google Scholar
  5. Foroughi-Wehr B., Friedt W., Wenzel G. 1982. On the genetic improvement of androgenic haploid formation in Hordeum vulgare L., Theor. Appl. Genet. 62:233–239CrossRefGoogle Scholar
  6. Gonzales M., Hernandez I., Jouve N., 1997. Analysis of anther culture response in hexaploid triticale. Plant Breed. 105:332–336Google Scholar
  7. Immonen S., Robinson J., 2000. Stress treatments and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci. 150:77–84CrossRefGoogle Scholar
  8. Liu W., Zheng M.Y., Polle E. A., Konzak C. F, 2002. Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis. Crop Science 42:686–692CrossRefGoogle Scholar
  9. Lazar M.D., Schaeffer G.W., Baenziger P.S., 1984. Cultivar and cultivar-environment effects on the development of callus and polyhaploid plants from anther cultures of wheat. Theor. Appl. Genet. 67:273–277.CrossRefGoogle Scholar
  10. Luckett D.J., Darvey N.L., 1992. Utilisation of microspore culture in wheat and barley improvement. Aust. J. Bot. 40:807–828CrossRefGoogle Scholar
  11. Liu W., Zheng M.Y., Polle E.A., Konzak C.F. 2002. Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis Crop Science 42:686–692CrossRefGoogle Scholar
  12. Marciniak K. 2001. Uzyskiwanie form haploidalnych i linii podwojonych haploidów pszenżyta heksaploidalnego (× Triticosecale Wittm.). Praca doktorska.Google Scholar
  13. Picard E., De Buyser J., 1977. High production of embryoids in anther culture of pollen derived homozygous spring wheat. Ann. Amelor. Plantes 25:483–488Google Scholar
  14. Schumann G., 1990. In vitro production of haploids in triticale. In Y.P.S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry. Vol.13 Wheat, pp. 382–402 Springer Verlag, HeidelbergGoogle Scholar
  15. Ślusarkiewicz-Jarzina A., Ponitka A., Adamski T., Surma M., 1996. Combining abilities of anther culture response in hexaploid triticale. J. Appl. Genet. 37 A: 208–212.Google Scholar
  16. Van Bergen S, Kottenhagen MJ, Van der Meulen RM, Wang M 1999. The role of abscisic acid in induction of androgenesis: a comparative study between Hordeum vulgare L cvs. Igri and Digger. J Plant Growth Regul 18: 135–143CrossRefGoogle Scholar
  17. Wang, M. Van Bergen S. Van Duijn, B. 2000. Insights into a key developmental switch and its importance for efficient plant breeding. Plant Physiol., Vol. 124, pp. 523–530CrossRefGoogle Scholar
  18. Wang P., Chen Y.R., 1983. Preliminary study on prediction of height of pollen H2 generation in winter wheat grown in the field. Acta Agron. Sin., 9,283–284.Google Scholar
  19. Wędzony M. 2003. Protocol for anther culture in hexaploid triticale (× Triticosecale Wittm.) Doubled haploid Production in Crop Plants 123–128.Google Scholar
  20. Zhang X.Q., Wang X.P., Ross K., Hu H., Gustafson J.P., 2001. Rapid introduction of disease resistance from rye into common wheat by anther culture of a 6 × triticale × nullitetrasomic wheat. Plant Breeding 120:39–42).CrossRefGoogle Scholar
  21. Zhuang J.J. and Jia X., 1983. Increasing differentiation frequencies in cheat pollen fallus. In: Hu H. & Vega MR (eds) Cell and Tissue Culture Techniques for Cereal Crop Improvement (p.431) Science Press, Beijing.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Magdalena Gut
    • 1
    Email author
  • Andrzej Bichoński
    • 2
  • Wojciech Mikulski
    • 3
  • Hanna Bielerzewska
    • 3
  1. 1.Department of Quality Evaluation and Cereals Breeding Methods KrakowKrakowPoland
  2. 2.Plant Breeding Station PolanowicePolanowicePoland
  3. 3.Plant Breeding Station SzelejewoPoznanPoland

Personalised recommendations