Cereal Research Communications

, Volume 34, Issue 2–3, pp 895–902 | Cite as

Potential uses of microsatellites in marker-assisted selection for improved bread-making quality in wheat

  • D. ObrehtEmail author
  • B. Kobiljski
  • S. Denčić
  • M. Djan
  • L. J. Vapa


Implementation of marker assisted selection (MAS) in conventional breeding programs could allow assessment of the genetic potential of specific genotypes prior to their phenotypic evaluation. Furthermore, it could identify important trait alleles or marker-trait associations for further determination of a precise position for the loci of interest. Potential uses of microsatellite markers in molecular evaluation of bread-making quality was tested in a sample of 69 wheat genotypes that were genotyped with 3 microsatellites linked to previously mapped QTLs for loaf volume and Hagberg falling number on chromosome 3A. A total of 19 alleles were found, with an average of 6.33 alleles per loci, and average PIC value of 0.40. Specific SSR alleles were tested for association with bread-making related parameters. The association study approach, which uses statistical analysis of marker and phenotypic data, showed significant association of a specific allele at the GWM674 locus with Hagberg falling number in wheat.

Index words

wheat SSRs bread-making quality 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Anderson, J.A., Sorels M.E., Tanksley S.D. (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci. 33: 453–459.CrossRefGoogle Scholar
  2. 2.
    Bai G., Das M.K., Carver B.F., Xu X., Krenzer E.G. (2004) Covariation for microsatellite marker alleles associated with Rht8 and coleoptile length in winter wheat. Crop Sci. 44: 1187–1194.CrossRefGoogle Scholar
  3. 3.
    Breseghello F., Sorrells M.E. (2005) Linkage disequilibrium and association mapping in soft winter wheat cultivars. Plant&Aminal Genomes XIII Conference, San Diego, USA, January 15–19. 2005., P361.Google Scholar
  4. 4.
    Campbell B.T., Baenziger P.S., Gill K.S., Eskridge K.M., Budak H., Erayman M., Dweikat I., Yen Y. (2003) Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A of wheat. Crop Sci. 43: 1493–1505.CrossRefGoogle Scholar
  5. 5.
    Clarke B.C., Phongkham T., Gianibelli M.C., Beasley H., Bekes F. (2003) The characterisation and mapping of a family of LMW-gliadin genes: effect on dough properties and bread volume. Theor. Appl. Genet. 106: 629–635.CrossRefGoogle Scholar
  6. 6.
    Eisemann B., Banks P., Butler D., Christopher M., DeLacy I., Jordan D., Mace E., McGowan P., McIntyre, Poulsen D., Rodgers D. and Sheppard J. (2004) Pedigree-based genome mapping for marker-assisted selection and recurrent parent recovery in wheat and barley. 4th Inter. Crop Sci. Congress, Brisbane, Australia.
  7. 7.
    Gebhardt C., Ballvora A., Walkemeier B., Oberhagemann P., Schuler K. (2004) Assessing genetic potential in germplasm collections of crop plants by marker-trait association: a case study for potatoes with quantitative variation of resistance to late blight and maturity type. Molecular Breeding 13: 93–102.CrossRefGoogle Scholar
  8. 8.
    Gross C., Gay G., Perretant M.-R., Gervais L., Bernard M., Dedryver F., Charmet G. (2002) Study of the relationship between pre-harvest sprouting and grain colour by quantitative trait loci analysis in white x red grain bread-wheat cross. Theor. Appl. Genet. 104: 39–47.CrossRefGoogle Scholar
  9. 9.
    Hogg A.C., Beecher B., Matrin J.M., Meyer F., Talbert L., Lanning S., Giroux M.J. (2005) Hard wheat milling and bread baking traits affected by the seed-specific overexpression of puroindolines. Crop Sci. 45: 871–878.CrossRefGoogle Scholar
  10. 10.
    ICC (1999) Standard Methods (Complete Edition including 1/7 Supplements). International Association of Cereal Science and Technology, Schwechat, Austria.Google Scholar
  11. 11.
    Ivandić V., Thomas W.T.B., Nevo E., Zhang Z., Forster B.P. (2003) Associations of simple sequence repeats with quantitative trait variation including biotic and abiotic stress tolerance in Hordeum spontaneum. Plant Breed. 122: 300–304.CrossRefGoogle Scholar
  12. 12.
    Kobiljski B., Denčić S., Hristov N., Mladenov N, Quarrie S., Stephenson P. and Kirby J. (2005) The potential of microsatellites for marker-assisted breeding for improved grain yield in wheat. Proc. 7 th Inter. Wheat Conf., Mar Del Plata, Argentina. pp.100.Google Scholar
  13. 13.
    Law C.N., Bhandari D.G., Salmon S.E., Greenwell P.W., Foot I.M., Cauvain S.P., Sayers E.J., Worland A.J. (2005) Novel genes on chromosome 3A influencing bread-making quality in wheat, including a new gene for loaf volume, Lvl 1. J. Cereal Sci. 41: 317–326.CrossRefGoogle Scholar
  14. 14.
    Mansour K. (1993) Sprout damage in wheat and its effect on wheat flour products. pp 8–9. In: Walker-Simons M.K., Ried J.L. (eds) Preharvest sprouting in cereals. Amer Assn of Cereal Chemists, USA.Google Scholar
  15. 15.
    Plaschke J., Ganal M. W., Roder M. S. (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91: 1001–1007.CrossRefGoogle Scholar
  16. 16.
    Roder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M-H., Leroy P., Ganal M.W. (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.PubMedPubMedCentralGoogle Scholar
  17. 17.
    Sanguinetti C.J., Dias Neto E., Simpson A.J.G. (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17: 915–919.Google Scholar
  18. 18.
    Schmidt A.L., Chunji L., Martin D., Kelly A., McIntyre L. (2004) Molecular markers for selected quality traits in Australian hexaploid bread wheat. New directions for a diverse planet: Proceedings for the 4th International Crop Science Congress, Brisbane, Australia, 26 September–1 October 2004.
  19. 19.
    Shah M.M., Gill K.S., Baenziger P.B., Yen Y., Kaeppler S.M., Ariyarathne H.M. (1999) Molecular mapping of loci for agronomic traits on chromosome 3A of bread wheat. Crop Sci. 39: 1728–1732.CrossRefGoogle Scholar
  20. 20.
    Simko I. (2004) One potato, two potato: Haplotype association mapping in autotetraploids. Trends in Plant Science 9: 441–448.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • D. Obreht
    • 1
    Email author
  • B. Kobiljski
    • 1
    • 2
  • S. Denčić
    • 1
    • 2
  • M. Djan
    • 1
  • L. J. Vapa
    • 1
  1. 1.Faculty of Natural SciencesInstitute of Field and Vegetable CropsNovi SadSerbia and Montenegro
  2. 2.Institute of Field and Vegetable CropsNoviSerbia and Montenegro

Personalised recommendations