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Cereal Research Communications

, Volume 33, Issue 4, pp 793–800 | Cite as

A high-molecular-weight glutenin subunit gene 1DX2.1 from Xinjiang rice wheat

  • Qian-Tao Jiang
  • Yu-Ming Wei
  • Ji-Rui Wang
  • Ze-Hong Yan
  • You-Liang ZhengEmail author
Article
  • 1 Downloads

Abstract

Xinjiang rice wheat (Triticum petropavlovskyi Udacz. et Migush, 2n=6x=42, AABBDD) is one of the endemic Chinese wheats, only distributing in Xinjiang and Xizang (Tibet), China. A novel high-molecular-weight (HMW) glutenin subunit gene 1DX2.1 was isolated and characterized from Xinjiang rice wheat accession Daomai2. The complete open reading frame (ORF) of 1Dx2.1 is 2508 bp, encoding 836 amino acids. The primary structure of 1DX2.1 consists of three distinct domains, a non-repetitive N-terminal domain with 89 residues, a non-repetitive C-terminal domain with 42 residues and a large central repetitive domain with 684 residues. In the N-terminal of 1DX2.1, there is an R (arginine) at position 75, whereas there is a Q (glutamine) in other known x-type subunits. Four cysteine residues are observed in 1DX2.1 with three in the N-terminal region and one in the C-terminal region. The number and distribution of cysteines in 1DX2.1 are identical to those in x-type subunits except for 1DX5, which possesses an extra cysteine residue. Differences between the repetitive domain of 1DX2.1 and those of known HMW subunits resulted from substitutions, insertions or/and deletions involving single or more amino acid residues. The phylogenetic tree, which was constructed on the basis of amino acid sequences, and indicated that 1DX2.1 was highly related to 1Dx2.lt, then to 1DX2 and 1DX5.

Keyword

high-molecular-weight glutenin subunit gene sequence analysis Triticum petropavlovsky 

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© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Qian-Tao Jiang
    • 1
  • Yu-Ming Wei
    • 1
  • Ji-Rui Wang
    • 1
  • Ze-Hong Yan
    • 1
  • You-Liang Zheng
    • 1
    Email author
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityDujiangyanChina

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