A high-molecular-weight glutenin subunit gene 1DX2.1 from Xinjiang rice wheat
- 1 Downloads
Xinjiang rice wheat (Triticum petropavlovskyi Udacz. et Migush, 2n=6x=42, AABBDD) is one of the endemic Chinese wheats, only distributing in Xinjiang and Xizang (Tibet), China. A novel high-molecular-weight (HMW) glutenin subunit gene 1DX2.1 was isolated and characterized from Xinjiang rice wheat accession Daomai2. The complete open reading frame (ORF) of 1Dx2.1 is 2508 bp, encoding 836 amino acids. The primary structure of 1DX2.1 consists of three distinct domains, a non-repetitive N-terminal domain with 89 residues, a non-repetitive C-terminal domain with 42 residues and a large central repetitive domain with 684 residues. In the N-terminal of 1DX2.1, there is an R (arginine) at position 75, whereas there is a Q (glutamine) in other known x-type subunits. Four cysteine residues are observed in 1DX2.1 with three in the N-terminal region and one in the C-terminal region. The number and distribution of cysteines in 1DX2.1 are identical to those in x-type subunits except for 1DX5, which possesses an extra cysteine residue. Differences between the repetitive domain of 1DX2.1 and those of known HMW subunits resulted from substitutions, insertions or/and deletions involving single or more amino acid residues. The phylogenetic tree, which was constructed on the basis of amino acid sequences, and indicated that 1DX2.1 was highly related to 1Dx2.lt, then to 1DX2 and 1DX5.
Keywordhigh-molecular-weight glutenin subunit gene sequence analysis Triticum petropavlovsky
Unable to display preview. Download preview PDF.
- Gupta, R. B., and F. MacRitchie. 1994. Allelic variation of glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheat. I. Its additive and interaction effects on dough properties. J. Cereal Sci. 19:9–17.Google Scholar
- Halford, N.G, J. Forde, O.D. Anderson, F.C. Greene, P.R. Shewry. 1987. The nucleotide and deduced amino acid sequence of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosome 1A and 1D. Theor. Appl. Genet. 75:117–126.CrossRefGoogle Scholar
- Halford, N.G., J. M. Field, H. Blair, P. Urwin, K. Moore, L. Robert, R. Thompson, R. B. Flavell, A.S. Tatham, and P.R. Shewry. 1992. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat Triticum aestivum L.) indicates quantitative effects on grain quality. Theor. Appl. Genet. 83:373–378.CrossRefGoogle Scholar
- Mackie, A.M., P.J. Sharp, E.S. Lagudah. 1996. The nucleotide and derived amino acid sequence of a HMW glutenin gene from Triticum. tauschii and comparison with those from the of D. genome of bread wheat. J. Cereal Sci. 24:73–78.Google Scholar
- Nei, N., S. Kumar, 2000. Molecular evolution and phylogenetics. Oxford University Press, UK.Google Scholar
- Payne, P.I. and G.J. Lawrence. 1983. Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1, which code for the high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Comm. 11:29–35.Google Scholar
- Shewry, P.R., A.S. Tatham, P. Barro, and P. Lazzeri. 1995. Biotechnology of breadmaking: unraveling and manipulating the multi-protein gluten complex. Biotechnology. 13:1185–1190.Google Scholar
- Shewry, P.R, S.M. Gilbert, A.W.J. Savage, AS. Tatham, Y.-F. Wan, P.S. Belton, N. Wellner, R. D’Ovidio, F. Bekes, N.G. Halfád. 2003. Sequence and properties of HMW subunit lBx20 from pasta wheat (Triticum durum) which is assodated with poor end use properties. Theor. AppL Genet 106:744–750.CrossRefGoogle Scholar
- Wei, Y.M., Y.J.L. Zheng, D.C. Liu, Y.H. Zhou, X.J. Lan. 2002. HMW-glutenin and gliadin vatiations in Tibetan weedrace, Xinjiang rice wheat and Yunnan hulled wheat Genet Resour Crop Ev. 49:327–330.Google Scholar
- Wei, Y.M., Y.L. Zheng, D.C. Liu, Y.H. Zhou, D.C. Liu, X.J. Lan, Z.H. Yan, Z.Q. Zhang. 2001. Genetic divasity of Gli-1, Gli-2 and Glu-1 alleles among Chinese endemic wheats. Acta Bot Sin. 43:834–839Google Scholar
- Yan, Z., Y. Wan, K. Liu, Y.L. Zheng, D.W. Wang. 2002. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Sci Bull. 47:220–225.Google Scholar
- Yang, W.Y., C. Yen, J.L. Yang. 1992. Cytogenetic study on the origin of some special Chinese landraces of common wheat. Wheat Infor Serv. 75:14–20.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.