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Cereal Research Communications

, Volume 33, Issue 4, pp 727–732 | Cite as

Effect of 2,4-D Concentration on the Androgenic Response in Anther Culture of Barley

  • Hanna KruczkowskaEmail author
  • Helena Pawlowska
  • Barbara Skucińska
Article

Abstract

The aim of the study was to determine the effect of various 2,4-D concentrations on the androgenic response and plant development in anther culture of barley. Anthers were either plated directly on the FHG medium supplemented with 20 mg dm−3 of 2,4-D, or pretreated with 0.7 mol dm−3 mannitol and plated on an induction medium containing various concentrations of 2,4-D: 0.2, 2.0, or 20 mg dm−3. The concentration of 20 mg dm−3 was used for 3 days only; subsequently, anthers were transferred onto the medium containing 0.2 mg dm−3 of 2,4-D. It was shown that osmotic stress cannot be replaced with the high concentration of 2,4-D. The most numerous embryos, embryogenic calli, and plants (including green plants) were obtained with 0.7mol dm−3 mannitol pretreatment and 0.2 mg dm−3 of 2,4-D in the induction medium. The auxin concentration also affected the dynamics of embryogenic structure formation and plant development from embryogenic structures. The concentrations higher than 0.2 mg dm−3 slowed down embryogenic structure formation and plant development, and they increased the proportion of calli as compared to embryos in plant regeneration.

Key words

barley haploid anther culture 2,4-dichlorophenoxyacetic acid 

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References

  1. Cai Q., Szarejko I., Polok K., Maluszyński M. (1992). The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breeding 109: 218–226.CrossRefGoogle Scholar
  2. Hoekstra S., van Bergen S., van Brouwershaven I. R., Schilperoort R. A. Heidekamp F. (1996). The interaction of 2,4-D application and mannitol pretreatment in anther and microspore culture of Hordeum vulgare L. cv. Igri. J. Plant Physiol. 148: 696–700.CrossRefGoogle Scholar
  3. Hu H. (1997). In vitro induced haploids in wheat. In: In vitro Haploid Production in Higher Plants, vol. 4 (S. M. Jain, S. K. Sopory, R. E. Veilleux, eds.). Kluwer Acad. Publishers, Dordrecht: 73–97.CrossRefGoogle Scholar
  4. Indrianto A., Heberle-Bors E., Touraev A. (1999). Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores. Plant Sci. 143: 71–79.CrossRefGoogle Scholar
  5. Jähne-Gärtner A., Lörz H. (1996). In vitro regeneration systems of barley (Hordeum vulgare L.). Plant Tissue Culture and Biotechnology 2 (1): 11–23.Google Scholar
  6. Kasha K.J., Simion E., Oro R. Yao Q. A., Hu T. C, Carlson A. R. (2001). An improved in vitro technique for isolated microspore culture of barley. Euphytica 120: 379–385.CrossRefGoogle Scholar
  7. Kruczkowska H., Pawłowska H., Skucinska B. (2002). Influence of anther pretreatment on the efficiency of androgenesis in barley. J. Appl. Genet. 43: 287–296.PubMedGoogle Scholar
  8. Kuhlmann U., Foroughi-Wehr B. (1989). Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78–81.CrossRefGoogle Scholar
  9. Li H., Devaux P. (2001). Enhancement of microspore culture efficiency of recalcitrant barley genotypes. Plant Cell Rep. 20: 475–481.CrossRefGoogle Scholar
  10. Marsolais A. A., Kasha K. J. (1985). Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium. Can. J. Bot. 63: 2209–2218.CrossRefGoogle Scholar
  11. Ohnoutková L., Novotný J., Müllerová E., Vagera J., KuČera L. (2000). Is a cold pretreatment really needed for induction of in vitro androgenesis in barley and wheat? In: Biotechnological Approaches for Utilization of Gametic Cells (B. Bohanec, ed.). COST 824, Bled, Slovenia 1–5 July 2000: 33–37.Google Scholar
  12. Powell W. (1988). Diallel analysis of barley anther culture response. Genome 30: 152–157.CrossRefGoogle Scholar
  13. Szarejko I. (1991). Kultury pylnikowe jqczmienia (Hordeum vulgare L.) i ocena genetyczna podwojonych haploiddw (Anther culture of Hordeum vulgare L. and genetic evaluation of microspore derived doubled haploids). Prace Naukowe US 1232, Katowice: 1–124.Google Scholar
  14. Szarejko I. (2003). Anther culture for doubled haploid production in barley (Hordeum vulgare L). In: Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (eds), Doubled Haploid Production in Crop Plants. Kluwer Acad. Publ., the Netherlands: 35–42.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Hanna Kruczkowska
    • 1
    Email author
  • Helena Pawlowska
    • 1
  • Barbara Skucińska
    • 1
  1. 1.Department of Plant Breeding and Seed ScienceAgricultural University of CracowKrakówPoland

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