The barley Lent 1 gene promoter drives expression specifically in outer floret organs at anthesis in transgenic wheat
Genetic engineering of cereal crops could be refined and made more publicly acceptable by the use of promoters that direct transgene expression to particular organs at defined stages of plant development. A fusion between the barley Lem1 promoter and green fluorescence protein was used to transform bread wheat. Expression analyses showed that the Lem1 promoter is active in the organs surrounding the wheat floret at anthesis. It is not active in other vegetative and seed tissues. The organ specificity and developmental regulation of the Lem1 promoter suggest that it would be useful for engineering Fusarium head blight resistance in wheat.
Index wordsfloret development genetic transformation organ specific promoters Triticum aestivum L.
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