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Cereal Research Communications

, Volume 43, Issue 2, pp 236–248 | Cite as

Morpho-physiological and Molecular Variability in Salt Tolerant and Susceptible Popular Cultivars and their Derivatives at Seedling Stage and Potential Parental Combinations in Breeding for Salt Tolerance in Rice

  • K. ChattopadhyayEmail author
  • D. Nath
  • R. L. Mohanta
  • B. C. Marndi
  • D. P. Singh
  • O. N. Singh
Physiology

Abstract

Saltol, a major QTL for salt exclusion, was derived from ‘Pokkali’, a salt-tolerant rice cultivar. Apart from Pokkali, many genotypes with wide variation for salinity tolerance offer ample scope for identifying new genes or QTLs underlying various tolerance mechanisms. Such genes could be aggregated into high-yielding backgrounds to reinforce a breeding programme. To identify potential donors for salt tolerance and prospective parental combinations for developing high-yielding salt-tolerant cultivars, ten genotypes were subjected to salt stress and evaluated for morpho-physiological traits and marker-allele polymorphism in the Saltol-QTL region. Although the salt-susceptible high-yielding varieties clustered together in a 3-D plot, principal component analysis showed marked spatial isolation among the tolerant genotypes. Unlike Pokkali and its derivative FL496, Rahspunjar maintained a higher level of K+ despite high Na+ influx in shoots. The wider genetic distances observed at both phenotypic and genotypic levels suggest the possibility of getting transgressive segregants among the offspring of crosses between Rahspunjar and Gayatri or Swarna Sub1. Similarly, SR 26B, which coped with the stress by diluting the Na+ load by maintaining a higher growth rate, differed from Pokkali or Nona Bokra: these two coped with the stress by regulating the transmission of Na+ from roots to photosynthetically active sites. The F2:3 population derived from Savitri × SR 26B showed wide morpho-physiological diversity for salt tolerance. SR 26B was the most distant genotype from Pokkali in the Saltol QTL region and was salt tolerant despite the absence of Pokkali alleles in this region.

Keywords

salt tolerance principal component analysis ion exclusion Saltol QTL genetic diversity 

Abbreviations

SL

shoot length (cm)

RL

root length (cm)

DWS

dry weight of shoots per plant (mg)

DWR

dry weight of roots per plant (mg)

SNA

shoot Na+ concentration (µg/mg)

SNAC

shoot Na+ content (mg)

SK

shoot K+ concentration (µg/mg)

SKC

shoot K+ content per plant (mg)

SNA/K

shoot Na+/K+ ratio

RNA

root Na+ concentration (µg/mg)

RNAC

root Na+ content per plant (mg)

RK

root K+ concentration (µg/mg)

RKC

root K+ content per plant (mg)

RNA/K

root Na+/K+ ratio

TNAC

total Na+ content per plant (mg)

TKC

total K+ content per plant (mg)

PNA/K

plant Na+/K+ ratio

GTH

growth rate (%)

SES

standard evaluation system score (on a scale from 1 to 9)

CV

coefficient of variance

CD

critical difference

SEM

standard error of mean

MSS

mean sum of squares

SD

standard deviation

QTL

quantitative trait locia

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • K. Chattopadhyay
    • 1
    Email author
  • D. Nath
    • 1
  • R. L. Mohanta
    • 1
  • B. C. Marndi
    • 1
  • D. P. Singh
    • 1
  • O. N. Singh
    • 1
  1. 1.Central Rice Research InstituteCuttackIndia

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