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Cereal Research Communications

, Volume 43, Issue 1, pp 97–107 | Cite as

The Prevalence of Wheat and Spelt Sensitivity in a Randomly Selected Australian Population

  • N. T. Vu
  • J. Chin
  • J. A. Pasco
  • A. Kovács
  • L. W. Wing
  • F. BékésEmail author
  • D. A. I. Suter
Quality and Utilization

Abstract

To determine the prevalence of wheat sensitivity in a randomly selected Australian population, a study was carried out on sera samples of the Geelong Osteoporosis Study (GOS) age-stratified cohorts of men and women who were randomly selected from electoral rolls for the Barwon Statistical Division (n = 1145). The human sera were analysed by ELISA-based method using the ImmunoCAP 100 instrument (Phadia, Sweden). IgE from human sera were bound to the wheat (f4) and milk (f2) allergens that have been previously coated on the ImmunoCAPs. The number of IgE wheat and milk RAST positive individuals was determined. In order to relate the increased IgE immuno-reactivity to allergen symptoms, a questionnaire was established and sent to the blood donors and 974 individuals responded. Of these (n = 974) 147 individuals (15.1%) reported symptoms caused by wheat consumption, 179 (15.6%) and 112 (9.8%) sera showed RAST positive results (IgE > 60 response units) in wheat and milk RAST tests, respectively. However, only 2.5% of those participants with symptoms related to wheat had positive IgE values indicating that the relationship is complex: a large proportion (12.7%) of the investigated population might suffer from other wheat related disorders (i.e. not IgE mediated), such as celiac disease, non-celiac reaction to gluten, reaction to fructans for those with irritable bowel syndrome as well as other factors. For the 13.2% who showed raised IgE antibody levels without symptoms we postulate that these individuals have latent wheat sensitivity with the potential of developing symptoms sooner or later.

A comparative study to investigate the immune reactivity of human IgE against wheat and spelt antigens was carried out using the sera of 73 patients found to be RAST positive for wheat. Of these 63% (n = 50) showed a higher IgE immune reactivity against wheat, while 30% (n = 24) showed higher IgE response against spelt antigens, the remaining 7% have indifferent responses against both antigens. Since the provided Phadia wheat and spelt antigens used in this study originated from Europe, Australian wheat and spelt varieties were also used to prepare antigens in order to investigate the response of Australian sera to local wheats. It was found that the immune reactivity of IgE wheat positive sera from a normal Australian population is lower for spelts compared to wheats regardless of their origin but much lower against an Australian spelt containing a mutation in its expansin gene. Aclinical feeding trial would be necessary to confirm if this difference in immune reactivity between spelt and wheat is consistent with a difference in allergenicity.

Keywords

wheat related health disorders prevalence of wheat sensitivity IgE RAST test expansin 

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • N. T. Vu
    • 1
  • J. Chin
    • 1
    • 2
  • J. A. Pasco
    • 3
  • A. Kovács
    • 4
  • L. W. Wing
    • 5
  • F. Békés
    • 6
    Email author
  • D. A. I. Suter
    • 6
  1. 1.Faculty of Agriculture and EnvironmentUniversity of SydneyEveleighAustralia
  2. 2.Xion BiotechnologyRhodesAustralia
  3. 3.School of MedicineDeakin UniversityGeelongAustralia
  4. 4.Agricultural Institute Centre for Agricultural Research of HASMartonvásárHungary
  5. 5.Bondi JunctionSydneyAustralia
  6. 6.George Weston Foods P/LNorth RydeAustralia

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