Oxidative Stress of Maize Roots Caused by a Combination of both Salt Stress and Manganese Deprivation
Salt stress impaired Mn imbalance and resulted in accumulation of ROS, and caused oxidative stress to plants. However, very little is known about the oxidative damage of maize roots caused by exposure to a combination of both salt stress and Mn deprivation. Thus the main aim of this study was to determine the effects of a combination of salt stress and Mn deprivation on antioxidative defense system in maize roots. Maize plants were cultivated in Hoagland’s media. They were subjected to 80 mM NaCl administered in the Mn-present Hoagland’s or Mn-deficient Hoagland’s media for 14 days. The findings indicated that the growth and root activity of maize seedlings cultivated in a combination of both salt stress and Mn deprivation were significantly inhibited; the compatible solute accumulation, malondialdehyde, carbonyl, 8-OHdG, and ROS were higher than those of the individual salt stress or Mn deprivation as expected. Nevertheless, the antioxidative enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase, glutathione-S-transferase and antioxidants such as ascorbic acid, glutathione and thiol were lower than those of the individual salt stress or Mn deprivation. In view of the fact that salt stress impaired Mn nutrition of maize seedlings, the findings suggested that Mn deprivation at the cellular level may be a contributory factor to salt-induced oxidative stress and related oxidative damage of maize roots.
Keywordssalt stress maize manganese deprivation reactive oxygen species antioxidant defense system roots
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- Able A.J., Guest D.I., Sutherland M.W. 1998. Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoopspores of Phytophthora parasitica var. nicotianae. Plant Physiol 117:491–499.PubMedPubMedCentralCrossRefGoogle Scholar
- Arnon D.I., Hoagland D.R. 1940. Crop production in artificial solutions and in soil with special reference to factors affecting yields and absorption of inorganic nutrients. Soil Sci. 50:463–484.Google Scholar
- Crawford N.M., Kahn M.L., Leustek T., Long S.R. 2000. Nitrogen and sulfur. In: Buchanan B.B., Gruissem W., Jones R.L. (eds), Biochemistry and Molecular Biology of Plants. ASPP. Rockville, USA, pp. 786–849.Google Scholar
- Hoque M.A., Banu M.N.A., Okuma E., Amako K., Nakamura Y., Shimoishi Y., Murata Y. 2007b. Exogenous proline and glycinebe-taine increase NaCl-induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. J. Plant Physiol. 164:1457–1468.PubMedCrossRefGoogle Scholar
- Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.Google Scholar
- Marschner H. 1995. Mineral Nutrition of Higher Plants, 2nd ed. Academic Press, New York, USA.Google Scholar
- Noreen Z., Ashraf M., Akram N.A. 2010. Salt-induced regulation of some key antioxidant enzymes and physio-biochemical phenomena in five diverse cultivars of turnip (Brassica rapa L.). J. Agron. Crop. Sci. 196:273–285.Google Scholar
- Rodríguez-Serrano M., Romero-Puertas M.C., Zabalza A., Corpas F.J., Gómez M., Del Río L.A., Sandalio L. 2006. Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell Environ. 29:1532–1544.PubMedCrossRefGoogle Scholar
- Zhao K.F., Li F.Z. 1999. Halophytes in China. Science Press, Beijing, China.Google Scholar
- Zou Q. 1995. Guide to Physiological and Biochemical Experiments. Agriculture Press, Beijing, China, pp. 30–31.Google Scholar
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