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Cereal Research Communications

, Volume 42, Issue 4, pp 568–577 | Cite as

Oxidative Stress of Maize Roots Caused by a Combination of both Salt Stress and Manganese Deprivation

  • H. Q. Zhao
  • L. Wang
  • J. Hong
  • X. Y. Zhao
  • X. H. Yu
  • L. Sheng
  • C. Z. Hang
  • Y. Zhao
  • A. A. Lin
  • W. H. SiEmail author
  • F. S. HongEmail author
Physiology
First Online:

Abstract

Salt stress impaired Mn imbalance and resulted in accumulation of ROS, and caused oxidative stress to plants. However, very little is known about the oxidative damage of maize roots caused by exposure to a combination of both salt stress and Mn deprivation. Thus the main aim of this study was to determine the effects of a combination of salt stress and Mn deprivation on antioxidative defense system in maize roots. Maize plants were cultivated in Hoagland’s media. They were subjected to 80 mM NaCl administered in the Mn-present Hoagland’s or Mn-deficient Hoagland’s media for 14 days. The findings indicated that the growth and root activity of maize seedlings cultivated in a combination of both salt stress and Mn deprivation were significantly inhibited; the compatible solute accumulation, malondialdehyde, carbonyl, 8-OHdG, and ROS were higher than those of the individual salt stress or Mn deprivation as expected. Nevertheless, the antioxidative enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase, glutathione-S-transferase and antioxidants such as ascorbic acid, glutathione and thiol were lower than those of the individual salt stress or Mn deprivation. In view of the fact that salt stress impaired Mn nutrition of maize seedlings, the findings suggested that Mn deprivation at the cellular level may be a contributory factor to salt-induced oxidative stress and related oxidative damage of maize roots.

Keywords

salt stress maize manganese deprivation reactive oxygen species antioxidant defense system roots 
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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • H. Q. Zhao
    • 1
    • 2
  • L. Wang
    • 3
  • J. Hong
    • 1
  • X. Y. Zhao
    • 1
  • X. H. Yu
    • 1
  • L. Sheng
    • 1
  • C. Z. Hang
    • 1
  • Y. Zhao
    • 1
  • A. A. Lin
    • 1
  • W. H. Si
    • 4
    Email author
  • F. S. Hong
    • 1
    Email author
  1. 1.Medical College of Soochow UniversitySuzhouChina
  2. 2.College of Life SciencesAnhui Agriculture UniversityHefeiChina
  3. 3.Library of Soochow UniversitySuzhouChina
  4. 4.Suzhou Polytechnical College of AgricultureSuzhouChina

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