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Cereal Research Communications

, Volume 42, Issue 3, pp 503–513 | Cite as

Molecular Characterization of a Novel HMW Glutenin Subunit Dx2.3*t from Aegilops tauschii

  • B. Feng
  • Z. B. Xu
  • X. Wang
  • F. Jiang
  • G. J. Zhao
  • C. Xiang
  • T. WangEmail author
Open Access
Quality and Utilization

Abstract

High molecular weight (HMW) glutenin subunits are important seed storage proteins in wheat and its related species. Novel HMWglutenin subunits in Aegilops tauschii accession of TA2484 were detected and characterized. SDS-PAGE analysis revealed the y-type subunit from TA2484 displayed similar electrophoretic mobility compared to that of 1Dy12 subunit. However, the electrophoretic mobility of x-type subunit was faster than that of 1Dx2 subunit. The primary structure of the two cloned subunits from TA2484 was similar to that of the x- and y-type subunits reported before. However, the 148 residues of the x-type subunit, which contained the sequence element GHCPTSLQQ, in the middle of the repetitive domain was quite different from other x-type subunits. Moreover, the 68 residues in this region were identical to those of the y-type subunits from the same accession. Consequently, 1Dx2.3*t (x-type subunit of TA2484) contains an extra cystenin residue located at the repetitive domain, which is novel compared to the x-type subunits reported so far. Phylogenetic analysis indicated that two subunits from accession TA2484 were in the x- and y-type subunit cluster, but bootstrapping value of 100% gave high support for the spilt between two subunits (1Dx2.3*t and 1Dy12.3*t) and their alleles, respectively. A hypothesis on the genetic mechanism generating this novel sequence of 1Dx2.3*t subunit is suggested.

Keywords

Aegilops tauschii HMW glutenin subunit 1Dx2.3*t phylogenetic analysis 

Supplementary material

42976_2014_4203503_MOESM1_ESM.pdf (1.2 mb)
Supplementary material, approximately 1282 KB.

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • B. Feng
    • 1
  • Z. B. Xu
    • 1
  • X. Wang
    • 1
  • F. Jiang
    • 1
    • 2
  • G. J. Zhao
    • 1
    • 2
  • C. Xiang
    • 1
    • 2
  • T. Wang
    • 1
    Email author
  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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