Cereal Research Communications

, Volume 42, Issue 2, pp 338–345 | Cite as

Influence of Precipitation Amount during Grain Filling on Nitrogen Uptake and Grain Yield of Spring Barley Fertilized by Ammonium Injection

  • O. SedlářEmail author
  • J. Balík
  • J. Černý
  • L. Peklová
  • K. Kubešová


The aim of this study was to observe the influence of the amount of precipitation during the grain filling period on nitrogen uptake and grain yield of spring barley fertilized by ammonium injection. Conventional nitrogen fertilization before sowing and CULTAN (Controlled Uptake Long Term Ammonium Nutrition) injection fertilization were compared during a 5-year small-plot field experiment under the conditions of Central Europe. In contrast to conventional nitrogen fertilization, with the CULTAN treatment there was observed no significantly negative effect of below-average precipitation during the grain filling period on post-heading (BBCH 51) nitrogen uptake from the soil with applications of 80 and 130 kg N.ha−1, grain yield, nitrogen uptake efficiency (NUpE) and thousand-grain weight with an application of 80 kg N.ha−1, nor there was a significantly positive effect on the contribution of nitrogen translocation to total nitrogen in the grain with an application of 130 kg N.ha−1. CULTANtreated plants achieved a significantly higher harvest index than conventionally treated plants with an application of 80 kg N.ha−1. Below average precipitation during the grain filling period had a significantly negative effect on nitrogen use efficiency (NUE) with both treatments. Using the CULTAN treatment leads to significantly lower flag leaf area compared to conventional treatment with an application of 80 kg N.ha−1. The application of 130 kg N.ha−1 brings no benefits to spring barley production.


drought stress water deficit remobilization Hordeum vulgare senescence 


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  1. Bahrani, A., Abad, H.H.S., Aynehband, A. 2011. Nitrogen remobilization in wheat as influenced by nitrogen application and post-anthesis water deficit during grain filling. African J. of Biotechnology 10:10585–10594.CrossRefGoogle Scholar
  2. Carfagna, S., Vona, V., Di Martino, V., Esposito, S., Rigano, C. 2011. Nitrogen assimilation and cysteine biosynthesis in barley: Evidence for root sulphur assimilation upon recovery from N deprivation. Environ. and Exp. Bot. 1:18–24.CrossRefGoogle Scholar
  3. Gaju, O., Allard, V., Martre, P., Snape, J.W., Heumez, E., Le Gouis, J., Moreau, D., Bogard, M., Griffiths, S., Orford, S., Hubbart, S., Foulkes, M.J. 2011. Identification of traits to improve the nitrogen-use efficiency of wheat genotypes. Field Crops Res. 123:139–152.CrossRefGoogle Scholar
  4. Guo, S., Zhou, Y., Shen, Q., Zhang, F. 2007. Effect of ammonium and nitrate nutrition on some physiological processes in higher plants — Growth, photosynthesis, photorespiration, and water relations. Plant Biol. 9:21–29.CrossRefGoogle Scholar
  5. Huang, M.L., Deng, X.P., Zhao, Y.Z., Zhou, S.L., Inanaga, S., Yamada, S., Tanaka, K. 2007. Water and nutrient use efficiency in diploid, tetraploid and hexaploid wheats. J. of Integrative Plant Biol. 49:706–715.CrossRefGoogle Scholar
  6. Inagaki, M.N., Mori, M., Nachit, M.M. 2010. Yield comparison for synthetic-derived bread wheat genotypes with different water uptake abilities under increasing soil water deficits. Cereal Res. Commun. 38:497–505.CrossRefGoogle Scholar
  7. Kaur, V., Behl, R.K. 2010. Grain yield in wheat as affected by short periods of high temperature, drought and their interaction during pre- and post-anthesis stages. Cereal Res. Commun. 38:514–520.CrossRefGoogle Scholar
  8. Kilic, H., Yagbasanlar, T. 2010. The effect of drought stress on grain yield, yield components and some quality traits of durum wheat (Triticum turgidum ssp. durum) cultivars. Not. Bot. Horti. Agrobo. 38:164–170.Google Scholar
  9. Kozlovský, O., Balík, J., Černý, J., Kulhánek, M., Kos, M., Prášilová, M. 2009. Influence of nitrogen fertilizer injection (CULTAN) on yield, yield components and quality of winter wheat grain. Plant Soil Environ. 55:536–543.CrossRefGoogle Scholar
  10. Krček, M., Slamka, P., Olšovská, K., Brestič, M., Benčíková, M. 2008. Reduction of drought stress effect in spring barley (Hordeum vulgare L.) by nitrogen fertilization. Plant Soil Environ. 54:7–13.CrossRefGoogle Scholar
  11. Ladha, J.K., Pathak, H., Krupnik, T.J., Six, J., van Kessel, C. 2005. Efficiency of fertilizer nitrogen in cereal production: Retrospects and prospects. Adv. Agron. 87:58–156.Google Scholar
  12. Li, A.G., Hou, Y.S., Wall, G.W., Trent, A., Kimball, B.A., Pinter, P.J. 2000. Free-air CO2 enrichment and drought stress effects on grain filling rate and duration in spring wheat. Crop Sci. 40:1263–1270.CrossRefGoogle Scholar
  13. Lopes, M.S., Nogues, S., Araus, J.L. 2004. Nitrogen source and water regime effects on barley photosynthesis and isotope signature. Functional Plant Biol. 31:995–1003.CrossRefGoogle Scholar
  14. Mori, M., Inagaki, M.N. 2012. Root development and water-uptake under water deficit stress in drought-adaptive wheat genotypes. Cereal Res. Commun. 40:44–52.CrossRefGoogle Scholar
  15. Pask, A.J.D., Sylvester-Bradley, R., Jamieson, P.D., Foulkes, M.J. 2012. Quantifying how winter wheat crops accumulate and use nitrogen reserves during growth. Field Crops Res. 126:104–118.CrossRefGoogle Scholar
  16. Peklová, L., Balík, J., Kozlovský, O., Sedlář, O., Kubešová, K. 2012. Influence of injection nitrogen fertilization on yield and seed composition of winter oilseed rape (Brassica napus L.). Plant Soil Environ. 58:508–513.CrossRefGoogle Scholar
  17. Samarah, N.H., Alqudah, A.M., Amayreh, J.A., McAndrews, G.M. 2009. The effect of late-terminal drought stress on yield components of four barley cultivars. J. of Agron. and Crop Sci. 195:427–441.CrossRefGoogle Scholar
  18. Schittenhelm, S., Menge-Hartmann, U. 2006. Yield formation and plant metabolism of spring barley in response to locally injected ammonium. J. of Agron. and Crop Sci. (Zeitschrift für Acker und Pflanzenbau) 192:434–444.CrossRefGoogle Scholar
  19. Sedlář, O., Balík, J., Kozlovský, O., Peklová, L., Kubešová, K. 2011a. Impact of nitrogen fertilizer injection on grain yield and yield formation of spring barley (Hordeum vulgare L.). Plant Soil Environ. 57:547–552.CrossRefGoogle Scholar
  20. Sedlář, O., Kozlovský, O., Peklová, L., Kubešová, K. 2011b. Vliv injektážní aplikace dusíku na vývoj porostu jarního ječmene (Effect of nitrogen injection application on growth progress of spring barley). Agrochémia (Agrochemistry) 15:11–14. (in Czech)Google Scholar
  21. Smirnoff, N. 1998. Plant resistance to environmental stress. Curr. Opin. Biotech. 9:214–219.CrossRefGoogle Scholar
  22. Sommer, K. 2005. The CULTAN-fertilization. Verlag Th. Mann, Gelsenkirchen, Germany, 218 pp. (in German)Google Scholar
  23. Spiertz, J.H.J. 2010. Nitrogen, sustainable agriculture and food security. A review. Agron. Sustain. Dev. 30:43–55.CrossRefGoogle Scholar
  24. Užík, M., Žofajová, A. 2012. Progress in accumulation and translocation of nitrogen in winter wheat cultivars released in the years 1921–2003. Cereal Res. Commun. 40:135–146.CrossRefGoogle Scholar
  25. Yang, J.C., Zhang, J.H. 2006. Grain filling of cereals under soil drying. New Phytol. 169:223–236.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • O. Sedlář
    • 1
    Email author
  • J. Balík
    • 1
  • J. Černý
    • 1
  • L. Peklová
    • 1
  • K. Kubešová
    • 1
  1. 1.Department of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural ResourcesCzech University of Life Sciences PraguePraha 6 — SuchdolCzech Republic

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