Cereal Research Communications

, Volume 41, Issue 3, pp 440–447 | Cite as

Prediction of Protein Fractions Distribution in Wheat (Triticum aestivum L.) Mill Products

  • L. Hřivna
  • L. Homola
  • I. BurešováEmail author
  • P. Smutná
Quality and Utilization


The research was conducted to describe distribution of protein fractions in wheat (Triticum aestivum L.) mill products (semolina, flour and bran) and evaluate the possibility of prediction of protein fractions distribution from values of bread-making quality (protein and gluten content, Zeleny sedimentation volume) evaluated on wholemeal and specific flour. The content of protein fractions was determined by size-exclusion high performance liquid chromatography. Significantly highest glutenin content was found in flour (5.01%). The investigated mill products did not differ in gliadin content, the content of albumin/globulin fraction reached the highest values in bran (3.60%). The model of prediction of glutenin and gliadin content in mill products explained 31–62% and 83–92% of the original variability. The protein fractions distribution in wheat mill products could be satisfactorily predicted from known values of protein and gluten content evaluated on wholemeal and Zeleny sedimentation volume evaluated on specific flour.


Triticum aestivum L. protein fractions flour semolina bran 


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  1. Belderok, B., Mesdag, J., Donner, D.A. 2000. Bread-Making Quality of Wheat. A Century of Breeding in Europe. Part One: Developments in bread-making processes. Part Two: Breeding for bread-making quality in Europe. Kluwer Academic Publishers, Dordrecht, The Netherlands, 416 pp.Google Scholar
  2. Belitz, H.D., Grosch, W., Schieberle, P. 2009. Food Chemistry (4th ed.). Springer, Berlin, Germany, pp. 670–745.Google Scholar
  3. Burešová, I., Hrivna, L., Dvořáková, P., Sedláčková, I. 2012. Practical application of size-exclusion high performance liquid chromatography in wheat analysis. Cereal Res. Commun. 40:552–561.CrossRefGoogle Scholar
  4. Ciaffi, M., Tozzi, L., Lafiandra, D. 1996. Relationship between flour protein composition determined by size-exclusion high-performance liquid chromatography and dough rheological parameters. Cereal Chem. 73:346–351.Google Scholar
  5. Dachkevitch, T., Autran, J.C. 1989. Prediction of baking quality of bread wheats in breeding programs by SE — HPLC. Cereal Chem. 66:448–456.Google Scholar
  6. Dendy, D.A.V., Dobraszczyk, B.J. 2001. Cereals and Cereal Products: Chemistry and Technology. Aspen Publishers, Inc., Gaithersburg, Germany, 429 pp.Google Scholar
  7. Edwards, M.A., Osborne, B.G., Henry, R.J. 2008. Effect of endosperm starch granule size distribution on milling yield in hard wheat. J. Cereal Sci. 48:180–192.CrossRefGoogle Scholar
  8. Gianibelli, M.C., Laroque, O.R., MacRitchie, F., Wrigley, C.W. 2001. Biochemical, genetic and molecular characterization of wheat endosperm proteins. Cereal Chem. 78:635–646.CrossRefGoogle Scholar
  9. Goesaert, H., Brijs, K., Veraverbeke, W.S., Courtin, C.M., Gebruers, K., Delcour, J. A. 2005. Wheat flour constituents: How they impact bread quality, and how to impact their functionality. Trends in Food Science and Technology 16:12–30.CrossRefGoogle Scholar
  10. Greffeuille, V., Abecassis, J., Rousset, M., Oury, F.X., Faye, A., Bar L’Helgouac’h, C., Lullien-Pellerin, V. 2006. Grain characterization and milling behaviour of near-isogenic lines differing by hardness. Theor. Appl. Genet. 114:1–12.CrossRefGoogle Scholar
  11. Hamer, R.J., Hoseney, R.C. 1998. Interactions: The Key to Cereal Quality. American Association of Cereal Chemists, St. Paul, MN, USA, 173 pp.Google Scholar
  12. ICC 1994. ICC standard No. 155 Determination of wet gluten quantity and quality (Gluten Index ac. to Perten) of whole wheat meal and wheat flour (Triticum aestivum).Google Scholar
  13. ICC 2000. ICC standard No. 167 Determination of crude protein in grain and grain products for food and feed by the Dumas Combustion Principle.Google Scholar
  14. Inglett, G.E. 1974. Wheat: Production and Utilization. AVI Publishing Company, Inc., Westport, CT, USA, 500 pp.Google Scholar
  15. ISO 2007. ISO standard No. 5529 Wheat — Determination of the sedimentation index — Zeleny test.Google Scholar
  16. Labuschagne, M.T., Koen, E., Dessalegn, T. 2004. Use of size-exclusion high-performance liquid chromatography for wheat quality prediction in Ethiopia. Cereal Chem. 81:533–537.CrossRefGoogle Scholar
  17. Park, S.H., Wilson, J.D., Seabourn, B.W. 2009. Starch granule size distribution of hard red winter and hard red spring wheat: Its effect on mixing and breadmaking quality. J. Cereal Sci. 49:98–105.CrossRefGoogle Scholar
  18. Ranken, M.D., Kill, R.C., Baker, C. 1997. Food Industries Manual (24th ed.). Chapman and Hall, London, UK, pp. 172–210.CrossRefGoogle Scholar
  19. Shewry, P.R. 2009. Wheat. J. Exp. Bot. 60:1537–1553.CrossRefGoogle Scholar
  20. Wieser, H. 2007. Chemistry of gluten proteins. Food Microbiol. 24:115–119.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • L. Hřivna
    • 1
  • L. Homola
    • 1
  • I. Burešová
    • 2
    Email author
  • P. Smutná
    • 1
  1. 1.Faculty of AgronomyMendel University in BrnoBrnoCzech Republic
  2. 2.Department of Food Technology and Microbiology, Faculty of TechnologyTomas Bata University in ZlínZlínCzech Republic

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