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Cereal Research Communications

, Volume 41, Issue 3, pp 468–481 | Cite as

Application of a Rapid Electrophoresis Technique Analysing the Glutenin Subunit Composition of Wheat Genotypes

  • A. KovácsEmail author
  • M. Rakszegi
  • L. Láng
  • W. Ma
  • F. Békés
  • Z. Bedő
Quality and Utilization
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Abstract

The characterization of the old Hungarian varieties and landraces is an important part of Hungarian cereal research and breeding. Analysis of these germplasms with the most up-to-date methodologies results a broad scale of diversity of glutenin alleles, which proves their genetic heterogenicity. Exploitation of this attribute is an untapped possibility for developing modern varieties in our breeding programs. The previous research work revealed this diversity by SDS-PAGE analysis and MALDI-TOF technology. The powerful tool, the high throughput lab-on-a chip technique can facilitate the effectiveness of this function and decreases the cost of the analysis. This study demonstrates the application of this technique for analysing the old varieties. The allelic composition and their effects on bread making quality concluded by means of functional analysis.

Keywords

wheat glutenin lab-on-a-chip MALDI-TOF-MS SE-HPLC 

Abbreviations

HMW GS

high molecular weight glutenin subunits

LMW GS

low molecular weight glutenin subunits

LOC

lab-on-a-chip

MALDI-TOF-MS

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

SDS-PAGE

sodium dodecyl sulphate polyacrylamide gel electrophoresis

SE-HPLC

size-exclusion high performance liquid chromatography

UPP

unextractable polymeric protein

BEU

Brabender Extensograph Unit

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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Kovács
    • 1
    Email author
  • M. Rakszegi
    • 1
  • L. Láng
    • 1
  • W. Ma
    • 2
  • F. Békés
    • 3
  • Z. Bedő
    • 1
  1. 1.Centre for Agricultural Research of HASAgricultural InstituteMartonvásárHungary
  2. 2.State Agricultural Biotechnology CentreMurdoch UniversityPerthAustralia
  3. 3.FBFD PTY LTDBeecroftAustralia

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