Cereal Research Communications

, Volume 41, Issue 2, pp 304–315 | Cite as

Genetic Characterization of Turkish Cultivated Emmer Wheat [Triticum turgidum L. ssp. dicoccon (Schrank) Thell.] Landraces Based on Isoenzyme Analysis

  • Ö. ÖzbekEmail author
  • B. Göçmen Taşkin
  • S. Keskin Şan
  • V. Eser
  • O. Arslan
Quality and Utilization


Nineteen landrace populations of Turkish cultivated emmer wheat [Triticum turgidum L. ssp. dicoccon (Schrank) Thell.] were characterized in terms of three isoenzyme [Endopeptidase-1, Aminopeptidase-1 and Aminopeptidase-2] systems, by isoelectric focusing gel electrophoresis. For overall loci, the mean number of alleles and effective alleles were observed as 2.00 and 1.37, respectively. The mean value of gene diversity and average gene diversity, in overall loci, were detected as 0.23 and 0.07, respectively. Actual genetic differentiation and gene flow between different populations were calculated as 0.19 and 0.11, respectively. Pearson’s correlation and multiple regression analyses indicated that eco-geographical variables have significant effects on isoenzyme genetic diversity. Landraces that have desirable agronomical and immunological resistance traits that makes them adaptable to climate change and different eco-geographical conditions are important genetic resources to utilise for the improvement of future crops of modern wheat varieties. There is a need to assess the genetic structure and genetic composition of important agronomical characters and to determine the magnitude of the genetic diversity currently conserved in the germplasm of landraces, both in farm fields and in ex situ collections and finally, strategies for the effective use of landraces, particularly of emmer wheat, should be planned and implemented in Turkey were discussed.


landraces cultivated emmer wheat Triticum turgidum L. ssp. dicoccon genetic variation Endopeptidase-1 Aminopeptidase-1 Aminopeptidase-2 IEF 


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  1. Ainsworth, C.C. 1983. Genetic control of hexokinase isozyme in wheat. Genetic Res. 42:219–227.CrossRefGoogle Scholar
  2. Ainsworth, C.C., Gale, M.D., Baird, S. 1984a. The genetic control of grain esterase in hexaploid wheat I Allelic variation. Theor. Appl. Genet. 68:219–210.CrossRefGoogle Scholar
  3. Ainsworth, C.C., Johnson, H.M., Jackson, E.A., Miller, T.E., Gale, M.D. 1984b. The chromosomal locations of leaf peroxidase genes in hexaploid wheat, rye, and barley. Theor. Appl. Genet. 69:205–210.CrossRefGoogle Scholar
  4. Ainsworth, C.C., Doherty, P., Edwards, K.G.K., Martinenssen, R.A., Gale, M.D. 1985. Allelic variation at a-amylase loci in hexaploid wheat. Theor. Appl. Genet. 70:400–406.CrossRefGoogle Scholar
  5. Asins, M., Carbonell, E. 1987. Concepts involved in measuring genetic variability and its importance in conservation of plant genetic resources. Evolutionary Trends in Plants 1:51–62.Google Scholar
  6. Berthaud, J., Pressoir, G., Ramirez-Corona, F., Bellon, M.R. 2002. Farmers’ management of maize landrace diversity. A case study in Oaxaca and beyond. In: Proc. 7th Int. Symp. on the Biosafety of Genetically Modified Organisms, Beijing, China, pp. 66–73.Google Scholar
  7. Chojecki, A.J.S., Gale, M.D. 1982. Genetic control of glucose phosphate isomerase in wheat and related species. Heredity 49:339–349.CrossRefGoogle Scholar
  8. Corazza, L., Pasquini, M., Perrino, P. 1986. Resistance to rusts and powdery mildew in some strains of Triticum monococcum L. and Triticum dicoccum Schubler cultivated in Italy. Genetica Agraria 40:243–254.Google Scholar
  9. Crawford, N.G. 2010. SMOGD: software for the measurement of genetic diversity. Molecular Ecology Resources 10:556–557.CrossRefGoogle Scholar
  10. Damania, A.B., Srivastrava, J.P. 1990. Genetic resources for optimal input technology ICARDA’s perspectives. In: El-Bassam, N., Dambrot, M., Loughman, B.C. (eds), Genetic Aspects of Plant Mineral Nutrition. Kluwer, Dordrecht, The Netherlands, pp. 425–430.CrossRefGoogle Scholar
  11. Hart, G.E., Langston, J.P. 1977. Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39:263–277.CrossRefGoogle Scholar
  12. Hedge, S.G., Valkoun, J., Waines, J.G. 2000. Genetic diversity in wild wheats and goat grass. Theor. Appl. Genet. 101:309–316.CrossRefGoogle Scholar
  13. Jaaska, V. 1997. Isoenzyme differences between the wild diploid and tetraploid wheats. Genetic Resources and Crop Evolution 44:137–146.CrossRefGoogle Scholar
  14. Jakubziner, M.M. 1969. Immunity of different wheat species. Agric. Biol. 4:837–847.Google Scholar
  15. Jost, L. 2008. GST and its relatives do not measure differentiation. Molecular Ecology 17:4015–4026.CrossRefGoogle Scholar
  16. Koebner, R.M. 1987. Genetic control of dipeptidase in the Triticeae. Theor. Appl. Genet. 74:387–390.CrossRefGoogle Scholar
  17. Koebner, R.M.D., Miller, T.E., Snape, W.J., Law, C.N. 1988. Wheat endopeptidase: genetic control, polymorphism, intra-chromosomal gene location, and alien variation. Genome 30:186–192.CrossRefGoogle Scholar
  18. Koebner, R.M.D., Martin, P.K. 1989. Chromosomal control of wheat and its close relatives. Theor. Appl. Genet. 78:657–661.CrossRefGoogle Scholar
  19. Lewontin, R.C. 1974. The Genetic Basis of Evolutionary Change. Columbia Univ. Press, New York and London.Google Scholar
  20. Li, Y.C., Krugman, T., Fahima, T., Beiles, A., Korol, A.B., Nevo, E. 2001. Spatiotemporal allozyme divergence caused by aridity stress in a natural population of wild wheat, Triticum dicoccoides, at the Ammiad microsite, Israel. Theor. Appl. Genet. 102:853–864.CrossRefGoogle Scholar
  21. McMillin, D.E., Allan, R.E., Roberts, D.E. 1986. Association of an isozyme locus and strawbreaker foot root resistance derived from Aegiolps ventricosa in wheat. Genetics 86:44–54.Google Scholar
  22. Medlinger, S., Zohary, D. 1995. The extent and structure of genetic variation in species of the Sitopsis group of Aegilops. Heredity 74:616–627.CrossRefGoogle Scholar
  23. Nei, M. 1973. Analysis differentiation in wild barley. Evolution of gene diversity in subdivided populations. Proc. Natl Acad. Sci. USA 70:3321–3323.CrossRefGoogle Scholar
  24. Nevo, E., Golenberg, E.M., Beiles, A., Brown, A.H.D., Zohary, D. 1982. Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet. 62:241–254.CrossRefGoogle Scholar
  25. Nevo, E., Beiles, A., Kaplan, D., Golenberg, E.M., Olsvig-whittaker, L., Nave, Z. 1986. Natural selection of allozyme polymorphism: A microsite test revealing ecological genetic differentiation in wild barley. Evolution 40:13–20.CrossRefGoogle Scholar
  26. Nevo, E., Payne, P.I. 1987. Wheat storage proteins: diversity of HMW glutenin sub-units in wild emmer from Israel. Theor. Appl. Genet. 74:827–836.CrossRefGoogle Scholar
  27. Nevo, E. 1988. Genetic diversity in nature: patterns and theory. BMC Evolutionary Biology 23:217–246.CrossRefGoogle Scholar
  28. Nevo, E., Beiles, A., Krugman, T. 1988. Natural selection of allozyme polymorphisms: a microgeographical differentiation by edaphic, topographical, and temporal factors in wild emmer wheat (Triticum dicoccoides). Theor. Appl. Genet. 76:737–752.CrossRefGoogle Scholar
  29. Nevo, E., Beiles, A. 1989. Genetic diversity of wild emmer wheat in Israel and Turkey. Theor. Appl. Genet. 77:421–455.CrossRefGoogle Scholar
  30. Nevo, E., Noy-Meir, I., Beiles, A., Krugman, T., Agami, M. 1991. Natural selection of allozyme polymorphisms: Micro-geographical spatial and temporal ecological differentiations in wild emmer wheat. Israel Journal of Botany 40:419–449.Google Scholar
  31. Nevo, E. 2001. Evolution of genome-phenome diversity under environmental stress. Proc. of the National Academy of Sciences 98:6233–6240.CrossRefGoogle Scholar
  32. Nybom, H. 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13:1143–1155.CrossRefGoogle Scholar
  33. Oliver, R.E., Cai, X., Friesen, T.L., Halley, S., Stack, R.W., Xu, S.S. 2008. Evaluation of Fusarium head blight resistance in tetraploid wheat (Triticum turgidum L.). Crop Sci. 48:213–222.CrossRefGoogle Scholar
  34. Özbek, Ö., Taşkén Göçmen, B., Şan Keskin, S., Eser, V., Arslan, O. 2011. Gliadin polymorphism in Turkish cultivated emmer wheat [Triticum turgidum L. ssp. dicoccon (Schrank) Thell.] landraces. Plant Systematics and Evolution 296:121–135.CrossRefGoogle Scholar
  35. Özbek, Ö., Taşkén Göçmen, B., Şan Keskin, S., Eser, V., Arslan, O. 2012. High-molecular-weight glutenin subunit variation in Turkish emmer wheat [Triticum turgidum L. ssp. dicoccon (Schrank) Thell.] landraces. Plant. Syst. Evol. 298:1795–1804. DOI Scholar
  36. Pagnotta, M.A., Impiglia, A., Tanzarella, O.A., Nachit, M.M., Porceddu, E. 2004. Genetic variation of the durum wheat landrace Haurani from different agro-ecological regions. Genetic Resources and Crop Evolution 51:863–869.CrossRefGoogle Scholar
  37. Scarascia-Mugnozza, G.T. 1995. The protection of biodiversity and the conservation and use of genetic resources for food and agriculture: Potential and perspectives. 19th McDougall Memorial Lecture, FAO, Rome, Italy.Google Scholar
  38. Smith-Huerta, N.L., Huerta, A.J, Barnhart, D., Waines, J.G. 1989. Genetic diversity in wild diploid wheats Triticum monococcum var. boeticum and T. urartu (Poaceae). Theor. Appl. Genet. 78:260–264.CrossRefGoogle Scholar
  39. Tripp, R., Heide, W. 1996. The erosion of crop genetic diversity: Challenges, strategies and uncertainties. Natural Resources Perspectives. Number 7, March 1996.
  40. Vapa, L., Hart, G.E. 1987. Genetic variation in enzyme phenotypes among Yugoslav wheat cultivars. Plant Breeding 98:273–280.CrossRefGoogle Scholar
  41. Yeh, F.C., Yang, R.C., Boyle, T., Ye, Z.H., Mao, J.X. 1997. POPGENE (version 1.32): The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada.Google Scholar

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© Akadémiai Kiadó, Budapest 2013

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Authors and Affiliations

  • Ö. Özbek
    • 1
    Email author
  • B. Göçmen Taşkin
    • 2
  • S. Keskin Şan
    • 3
  • V. Eser
    • 3
  • O. Arslan
    • 4
  1. 1.Faculty of Art and Sciences, Department of BiologyHitit UniversityÇorumTurkey
  2. 2.Faculty of Art and Sciences, Department of BiologyMugla UniversityMuglaTurkey
  3. 3.Central Research Institute for Field CropsUlusTurkey
  4. 4.Faculty of Education, Department of BiologyGazi UniversityAnkaraTurkey

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