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Acta Biologica Hungarica

, Volume 66, Issue 1, pp 93–102 | Cite as

Identification of New SSR Markers Linked to Leaf Chlorophyll Content, Flag Leaf Senescence and Cell Membrane Stability Traits in Wheat under Water Stressed Condition

  • Mohamed N. BarakatEmail author
  • Mohamed Saleh
  • Abdullah A. Al-Doss
  • Khaled A. Moustafa
  • Adel A. Elshafei
  • Fahed H. Al-Qurainy
Open Access
Article

Abstract

Segregating F4 families from the cross between drought sensitive (Yecora Rojo) and drought tolerant (Pavon 76) genotypes were made to identify SSR markers linked to leaf chlorophyll content, flag leaf senescence and cell membrane stability traits in wheat (Triticum aestivum L.) under water-stressed condition and to map quantitative trait locus (QTL) for the three physiological traits. The parents and 150 F4 families were evaluated phenotypically for drought tolerance using two irrigation treatments (2500 and 7500 m3/ha). Using 400 SSR primers tested for polymorphism in testing parental and F4 families genotypes, the results revealed that QTL for leaf chlorophyll content, flag leaf senescence and cell membrane stability traits were associated with 12, 5 and 12 SSR markers, respectively and explained phenotypic variation ranged from 6 to 42%. The SSR markers for physiological traits had genetic distances ranged from 12.5 to 25.5 cM. These SSR markers can be further used in breeding programs for drought tolerance in wheat.

Keywords

Physiological traits QTL SSR markers Triticum aestivum 

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Mohamed N. Barakat
    • 1
    • 2
    Email author
  • Mohamed Saleh
    • 3
  • Abdullah A. Al-Doss
    • 1
  • Khaled A. Moustafa
    • 1
  • Adel A. Elshafei
    • 1
    • 4
  • Fahed H. Al-Qurainy
    • 3
  1. 1.Plant Production DepartmentCollege of Food and Agriculture Sciences, King Saud UniversityRiyadhSaudi Arabia
  2. 2.Biotechnology Laboratory, Crop Science Department, Faculty of AgricultureUniversity of AlexandriaAlexandriaEgypt
  3. 3.Botany and Microbiology Department, College of Pure ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Genetics and Cytology DepartmentGenetic Engineering and Biotechnology Division, National Research CentreEl-Dokki, CairoEgypt

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