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Acta Biologica Hungarica

, Volume 66, Issue 1, pp 52–65 | Cite as

Pretreatment with Polyamines Alleviate the Deleterious Effects of Diuron in Maize Leaves

  • Nuran DurmuşEmail author
  • Tuba Bekircan
Article
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Abstract

The effects of diuron, a photosystem II inhibiting herbicide, on lipid peroxidation, photosynthetic pigments, soluble protein, proline contents and some antioxidant enzymes in maize leaves were studied and protective effects of polyamines against diuron toxicity were investigated. Diuron significantly increased lipid peroxidation, suggesting oxidative damage in the plants and proline content, while it decreased total chlorophyll, carotenoid and soluble protein levels in the leaves during the experiments. Pretreatment with polyamines statistically decreased lipid peroxidation induced by diuron and spermine (SPM) proved to be the most effective polyamine. Also, pretreatment with polyamines significantly prevented the losses of total chlorophyll, carotenoid and soluble protein induced by diuron. On the other hand, pretreatment with polyamines significantly increased proline contents of the leaves in comparison with the leaves treated with diuron. Superoxide dismutase (SOD), guaiacol peroxidase (GPX) and glutathione reductase (GR) activities increased in the leaves treated with diuron while catalase (CAT ) activity decreased. Pretreatment with spermidine (SPD) did not change significantly SOD activity a. 24 an. 72 hrs of diuron treatment but prevented the increase in SOD activity induced by diuron a. 48 h. However, pretreatment with SPD increased GPX activity a. 24 h and GR activity a. 48 an. 72 hrs. CAT activity in the leaves pretreated with SPD was similar to that of the leaves treated with diuron. Pretreatment with SPM prevented the increase in SOD activity induced by diuron a. 48 h but significantly increased it a. 72 h of diuron treatment. However, pretreatment with SPM did not significantly change GPX and GR activities during the experiments but reversed the decrease in CAT activity induced by diuron a. 72 h. Pretreatment with putrescine (PUT) prevented the increase in SOD activity induced by diuron a. 48 an. 72 hrs while it increased GPX and GR activities a. 48 h of diuron treatment. Also, the decrease in CAT activity induced by diuron a. 72 h was completely prevented by PUT. It can be concluded that pretreatment of maize leaves with polyamines reduced the damage produced by diuron and the protective effects of polyamines against diuron toxicity were closely associated with antioxidant system.

Keywords

Diuron polyamines antioxidant enzymes proline lipid peroxidation 

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Arts and SciencesRecep Tayyip Erdogan UniversityRizeTurkey

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