The interaction between sodium chloride and Trichoderma harzianum (T24) on growth parameters, ion contents, MDA content, proline, soluble proteins as well as SDS page protein profile were studied in Vicia faba Giza 429. A sharp reduction was found in fresh and dry mass of shoots and roots with increasing salinity. Trichoderma treatments promoted the growth criteria as compared with corresponding salinized plants. The water content and leaf area exhibited a marked decrease with increasing salinity. Trichoderma treatments induced a progressive increase in both parameters. Both proline and MDA contents were increased progressively as the salinity rose in the soil. Trichoderma treatments considerably retarded the accumulation of both parameters in shoots and roots. Both Na+ and K+ concentration increased in both organs by enhancing salinity levels. The treatment with Trichoderma harzianum enhanced the accumulation of both ions. Exposure of plants to different concentrations of salinity, or others treated with Trichoderma harzianum produced marked changes in their protein pattern. Three types of alterations were observed: the synthesis of certain proteins declined significantly, specific synthesis of certain other proteins were markedly observed and synthesis of a set specific protein was induced de novo in plant treated with Trichoderma harzianum.
Altintas, S., Bal, U. (2008) Effects of the commercial product based on Trichoderma harzianum on plant, bulb and yield characteristics of onion. Sci. Hortic. 116, 219–222.
Aust, S. D., Morehouse, L. A., Thomas, C. (1985) Role of metals in oxygen radical reactions. J. Free Radic. Biol. Med. 1, 3–26.
Bates, L. S., Waldern, R. P., Teare, I. D. (1973) Rapid determination of free proline for water stress studies. Plant Soil 39, 205–207.
Bewlfey, J. D., Oliver, M. J. (1983) Responses to a changing environment at the molecular level: Does desiccation, modulate protein synthesis at the transcriptional or translational level in a tolerant plant? Current Topics Plant Biochem. Biophys. 2, 145–146.
Chang, Y. C., Baker, R., Kleifeld, O., Chet, I. (1986) Increased growth of plants in the presence of the biological control agent Trichoderma harzianum, Plant Disease 70, 145–148.
Egberongbe, H. O., Akintokun, A. K., Babalola, O. O., Bankole, M. O. (2010) The effect of Glomus mosseae and Tricoderma harizianum on proximate analysis of soybean (Glycine max. (L.) Merrill) Seed grown in sterilized and unsterilized soil. J. Agric. Extension Rural Development 2, 54–58.
FAO (2000) Global network on integrated soil management for sustainable use of salt effected soils, Available in: http://www.fao.org/ag/AGL/agll/spush/intro.htm.
Foyer, C. H., Kiddle, G., Antoniw, J., Bernard, S., Verrier, P. J., Pastori, G. M., Noctor, G. (2003) The role of antioxidant-mediated signal transduction during stress. Mol. Cell. Proteomics 2, 682.
Gachomo, E. W., Kotchoni, S. O. (2008) The use of T. harzianum and T. viride as potential biocontrol agents against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. Biotechnol. 7, 439–447.
Grattan, S. R., Grieve, C. M. (1999) Salinity–mineral nutrient relations in horticultural crops. Sci. Hortic. 78, 127–157.
Greenway, H., Munns, H. (1980) Mechanisms of salt tolerance in non halophytes. Annu. Rev. Plant Physiol. 31, 149–190.
Grichko, V. P., Glick, B. R. (2001) Amelioration of flooding stress by ACC deaminase containing plant growth-promoting bacteria. Plant Physiol. Biochem. 39, 11–17.
Harman, G. E., Mattick, L. R. (1976) Association of lipid oxidation with seed aging and death. Nature 260, 323–324.
Harman, G. E. (2000) Myths and dogmas of biocontrol–changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Disease 84, 377–393.
Harman, G. E., Björkman, T. (1998) Potential and existing uses of Trichoderma and Gliocladium for plant disease control and plant growth enhancement. In: Harman, G. E., Kubicek, C. P. (eds), Trichoderma and Gliocladium. 2, Taylor & Francis, London, United Kingdom, pp. 229–265.
Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast. 1. Kinetics and stiochiometry of fatty acid peroxidation. Arch. Bioch. Biophys. 125, 189–198.
Hong, Z. L., Lakkineni, K., Zhang, Z. M., Verma, D. P. S. (2000) Removal of feedback inhibition of delta (1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol. 122, 1129–1136.
Howell, C. R. (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis. 87, 4–10.
Khan, M. H., Panda, S. K. (2008) Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl salinity stress. Acta Physiol. Plant. 30, 91–98.
Laemmli, U. K. (1970) Cleavage of structure proteins during assembly of the head of bacteriophage T4. Nature 277, 680–685.
Lowery, O. H., Rosebrough, N. H., Farr, A. L., Randall, R. J. (1951) Protein measurements with the folin phenol reagent. J. Biol. Chem. 193, 291–297.
Mastouri, F., Björkman, T., Harman, G. E. (2010) Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathol. 100, 1213–1221.
Michal, S. G., Harman, E. (2008) The molecular basis of shoot responses of maize seedling to Trichoderma harizianum T22 inoculation of the root. Plant Physiol. 147, 2147–2163.
Munns, R., Termaat, A. (1986) Whole plant responses to salinity. Aust. J. Plant Physiol. 13, 143–160.
Navazio, L., Baldan, B., Moscatiello, R., Zuppini, A., Woo, S. L., Mariani, P., Lorito, M. (2007) Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride. BMC Plant Biol. 7, 41–49.
Shiqing, S., Shirong, G., Qingmao, S., Zhigang, Z. (2006) Physiological effects of exogenous salicylic acid on cucumber seedling under salt stress. Acta Hortic. Sin. 33, 68–72.
Sivritepe, N., Sivritepe, H. O., Eris, A. (2003) The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions. Sci. Hortic. 97, 229–237.
Tammam, A. A. (2003) Response of Vicia faba plants to the interactive effect of sodium chloride salinity and salicylic acid treatment. Acta Agron. Hungarica 51, 239–248.
Tucci, M., Ruocco, M., De Masi, L., De Palma, M., Lorito, M. (2011) The beneficial effect of Trichoderma spp. on tomato is modulated by the plant genotype. Mol. Plant Pathol. 12, 341–354.
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Barbetti, M. J., Li, H., Woo, S. L., Lorito, M. (2008) A novel role for R. Hermosa and others Trichoderma secondary metabolites in the interactions with plants. Physiol. Mol. Plant Pathol. 72, 80–86.
Wiersma, T. V., Bailey, T. B. (1975) Estimation of leaflet, trifoliate and total leaf area of soybean. Agron. J. 176, 26–30.
Williams, S., Twine, M. (1960) Flame photometric method for sodium, potassium and calcium. In: Peach, K., Tracey, M. V. (eds) Modern Methods of Plant Analysis. Vol. 5. Springer Verlag, Berlin, pp. 3–5.
The authors would like to thank Prof. Dr. M. A. A. Shaddad, Assuit University for his valuable advices and revising the manuscript.
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El-Baki, G.K.A., Mostafa, D. The Potentiality of Trichoderma Harzianum in Alleviation the Adverse Effects of Salinity in Faba Bean Plants. BIOLOGIA FUTURA 65, 451–468 (2014). https://doi.org/10.1556/ABiol.65.2014.4.9
- Lipid peroxidation