Acta Biologica Hungarica

, Volume 65, Issue 3, pp 319–330 | Cite as

Investigation of the Effects of Cadmium by Micro Analytical Methods on Lycopersicon esculentum Mill. Roots

  • G. ColakEmail author
  • M. C. Baykul
  • R. Gürler
  • E. Catak
  • N. Caner


The interactions between cadmium stress and plant nutritional elements have been investigated on complete plant or at the level of organs. This study was undertaken to contribute to the exploration of the physiological basis of cadmium phytotoxicity. We examined the changes in the nutritional element compositions of the root epidermal cells of the seedlings of Lycopersicon esculentum Mill. at the initial growth stages that is known as the most sensitive stage to the stress. Effects of cadmium stress on the seedlings of Lycopersicon esculentum Mill. were examined by EDX (Energy Dispersive X-Ray Microanalysis) assay performed with using low vacuum (≈ 24 Pascal) Scanning Electron Microscopy. In the analysis performed at the level of root epidermal cells, some of the macro- and micronutrient contents of the cells (carbon, oxygen, nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, copper, and zinc levels) were found to change when the applying toxic concentrations of cadmium. There was no change in the manganese and sodium content of the epidermal cells. It was concluded that the changes in nutritional element composition of the cells can be considered as an effective parameter in explaining the physiological mechanisms of cadmium-induced growth inhibition.


Lycopersicon esculentum cadmium toxicity plant nutrients SEM EDX 


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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • G. Colak
    • 1
    Email author
  • M. C. Baykul
    • 2
  • R. Gürler
    • 4
  • E. Catak
    • 1
  • N. Caner
    • 3
  1. 1.Science Faculty Department of BiologyEskisehir Osmangazi UniversityEskisehirTurkey
  2. 2.Science Faculty Department of PhysicsEskisehir Osmangazi UniversityEskisehirTurkey
  3. 3.Science Faculty Department of ChemistryEskisehir Osmangazi UniversityEskisehirTurkey
  4. 4.Metallurgy InstituteEskisehir Osmangazi UniversityEskisehirTurkey

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