Effects of Ascorbic Acid on Some Physiological Changes of Pepino (Solanum Muricatum Ait.) under Chilling Stress

Abstract

In this study, the changes caused by chilling stress on some physiological parameters of pepino (Solanum muricatum Ait.) plant and the effects of ascorbic acid (100 mM) applied exogenously on these changes were examined. For this purpose, the photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophylls and carotenoids), ascorbic acid, total phenolic compounds, malondialdehyde and proline contents in leaves of pepino taken on 5th and 10th days were determined. As a result of chilling stress, it was found that while the photosynthetic pigments and proline contents decreased in pepino leaves, the ascorbic acid, total phenolic compounds and malondialdehyde contents increased. In plants which were subjected to pre-treatment of ascorbic acid on the 10th day of stress, ascorbic acid and proline contents increased while a decrease was observed in malondialdehyde content, compared to stress group without pre-treated. This study may be important for explaining resistance induced by treatment of exogenous ascorbic acid in pepino exposed to chilling stress.

References

  1. 1.

    Ahumada, M., Cantwell, M. (1996) Postharvest studies on pepino dulce (Solanum muricatum Ait.): maturity at harvest and storage behavior. Postharvest Biol. Tec. 7, 129–136.

    Article  Google Scholar 

  2. 2.

    Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of proline for water-studies. Plant Soil. 39, 205–207.

    CAS  Article  Google Scholar 

  3. 3.

    Bravo, M. A., Arias, E. (1983) Cultivo del pepino dulce. antecedentes agronómicos y económicos. El Campesino. 114(3), 15–34.

    Google Scholar 

  4. 4.

    Bybordi, A. (2012) Effect of ascorbic acid and silicium on photosynthesis, antioxidant enzyme activity, and fatty acid contents in canola exposure to salt stress. J. Integrative Agri. 11, 1610–1620.

    CAS  Article  Google Scholar 

  5. 5.

    Cao, S., Cai, Y., Yang, Z., Zheng, Y. (2012) MeJA induces chilling tolerance in loquat fruit by regulating proline and γ-aminobutyric acid contents. Food Chem. 133, 1466–1470.

    CAS  Article  Google Scholar 

  6. 6.

    Chandler, S. F., Dodds, J. H. (1983) The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum. Plant Cell Rep. 2, 105–110.

    Article  Google Scholar 

  7. 7.

    De Kok, L. J., Graham, M. (1989) Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senesence. Plant Physiol. Biochem. 27, 203–209.

    Google Scholar 

  8. 8.

    Delauney, A. J., Verma, D. P. S. (1993) Proline biosynthesis and osmoregulation in plants. Plant J. 4, 215–223.

    CAS  Article  Google Scholar 

  9. 9.

    Erdal, S. (2012) Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress. Plant Physiol. Biochem. 57, 1–7.

    CAS  Article  Google Scholar 

  10. 10.

    Foyer, C. H., Vanacker, H., Goméz, L. D., Harbinson, J. (2002) Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: review. Plant Physiol. Biochem. 40, 659–668.

    CAS  Article  Google Scholar 

  11. 11.

    Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Narusaka, Y., Yamaguchi-Shinozaki, K., Shinozaki, K. (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling Networks. Curr. Opin. Plant Biol. 9, 436–442.

    Article  Google Scholar 

  12. 12.

    Griffith, M., Yaish, M. W. F. (2004) Antifreeze proteins in overwintering plants: a tale of two activities. Trends Plant Sci. 9, 399–405.

    CAS  Article  Google Scholar 

  13. 13.

    Guo, Z., Tan, H., Zhu, Z., Lu, S., Zhou, B. (2005) Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance. Plant Physiol. Biochem. 43, 955–962.

    CAS  Article  Google Scholar 

  14. 14.

    Hamilton, E. W., Heckathorn, S. A. (2001) Mitochondrial adaptations to NaCl complex I is protected by antioxidants and small heat shock proteins, whereas complex II is protected by proline and betaine. Plant Physiol. 126, 1266–1274.

    CAS  Article  Google Scholar 

  15. 15.

    Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast, I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 180–198.

    Article  Google Scholar 

  16. 16.

    Heerden, P. D. R., Krüger, G. H. J. (2002) Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean. J. Plant Physiol. 159, 1077–1086.

    Article  Google Scholar 

  17. 17.

    Holá, D., Kočová, M., Rothová, O., Wilhelmová, N., Benešova, M. (2007) Recovery of maize (Zea mays L.) inbreds and hybrids from chilling stress of various duration: Photosynthesis and antioxidant enzymes. J. Plant Physiol. 164, 868–877.

    Article  Google Scholar 

  18. 18.

    Kader, D. Z. A., Saleh, A. A. H., Elmeleigy, S. A., Dosoky, N. S. (2011) Chilling-induced oxidative stress and polyamines regulatory role in two wheat varieties. Jtusci. 5, 14–24.

    Google Scholar 

  19. 19.

    Kaur, G., Kumar, S., Thakur, P., Malik, J. A., Bhandhari, K., Sharma, K. D., Nayyar, H. (2011) Involvement of proline in response of chickpea (Cicer arietinum L.) to chilling stress at reproductive stage. Sci. Hortic. 128, 174–181.

    CAS  Article  Google Scholar 

  20. 20.

    Labudda, M. (2013) Lipid peroxidation as a biochemical marker for oxidative stress during drought. An effective tool for plant breeding. https://doi.org/www.e-wydawnictwo.eu/Document/Documentpreview/3342.

    Google Scholar 

  21. 21.

    Lattanzio, V., Lattanzio, V. M. T., Cardinali, A. (2006) Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Imperato, F. (ed.) Phytochemistry: Advances in Research. ISBN: 81-308-0034-9. India, pp. 23–67.

    Google Scholar 

  22. 22.

    Li, Q., Yu, B., Gao, Y., Dai, A. H., Bai, J. G. (2011) Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity. J. Plant Physiol. 168, 927–934.

    CAS  Article  Google Scholar 

  23. 23.

    Lichtenthaler, H. K., Wellburn, A. R. (1983) Determinations of total carotenoids and chlorophylls a and b of leaf exracts in different solvents. Biochem. Soc. Trans. 11, 591–592.

    CAS  Article  Google Scholar 

  24. 24.

    Mandal, S., Yadav, S., Yadav, S., Nema, R. K. (2009) Antioxidants: A review. J. Chem. Pharm. Res. 1, 102–104.

    Google Scholar 

  25. 25.

    Mukherjee, S. P., Choudhuri, M. A. (1983) Implication of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plantarum 58, 166–170.

    CAS  Article  Google Scholar 

  26. 26.

    Padda, M. S., Picha, D. H. (2008) Effect of low temperature storage on phenolic composition and antioxidant activity of sweetpotatoes. Postharvest Biol. Tec. 47, 176–180.

    CAS  Article  Google Scholar 

  27. 27.

    Perez-Ilzarbe, J., Hernandez, T., Estrella I., Vendrell M. (1997) Cold storage of apples (cv. Granny Smith) and changes in phenolic compounds. Z. Lebensm. Unters. Forsch. 204, 52–55.

    CAS  Article  Google Scholar 

  28. 28.

    Prohens, J., Ruiz, J. J., Nuez, F. (2000) Growing cycles for a new crop, the pepino, in the Spanish mediterranean. Acta Hort. 523, 53–58.

    Article  Google Scholar 

  29. 29.

    Ramakrishna, A., Ravishankar, G. A. (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 6, 1720–1731.

    CAS  Article  Google Scholar 

  30. 30.

    Rasheed, R., Wahid, A., Ashraf, M., Basra, S. M. A. (2010) Role of proline and glycinebetaine in improving chilling stresstolerance in sugarcane buds at sprouting. International J. Agric. Biol. 12, 1–8.

    CAS  Google Scholar 

  31. 31.

    Ruiz, J. J., Nuez, F. (1997) The pepino (Solanum muricatum Ait): an alternative crop for areas affected by moderate salinity. Hort. Sci. 32, 649–652.

    Article  Google Scholar 

  32. 32.

    Sevengor, S., Yasar, F., Kusvuran, S., Ellialtıoglu, S. (2011) The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. Afr. J. Agric. Res. 6, 4920–4924.

    Google Scholar 

  33. 33.

    Slinkard, K., Singleton, V. L. (1977) Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49–55.

    CAS  Google Scholar 

  34. 34.

    Smirnoff, N. (2000) Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr. Opin. Plant Biol. 3, 229–235.

    CAS  Article  Google Scholar 

  35. 35.

    Tambussi, E. A., Bartoli, C. G., Guiamet, J. J., Beltrano, J., Araus, J. L. (2004) Oxidative stress and photodamage at low temperatures in soybean (Glycine max L. Merr.) leaves. Plant Sci. 167, 19–26.

    CAS  Article  Google Scholar 

  36. 36.

    Thakur, P., Nayyar, H. (2013) Facing the cold stress by plants in the changing environment: sensing, signaling, and defending mechanisms. In: Tuteja, N., Gill, S. S. (eds) Plant Acclimation to Environmental Stress. Chapter 2. DOI 10.1007/978-1-4614-5001-6_2, © Springer Science+Business Media, New York, pp. 29–69.

    Chapter  Google Scholar 

  37. 37.

    Xu, S., Li, Y., Hu, J., Guan, Y., Ma, W., Zheng, Y., Zhu, S. (2010) Responses of antioxidant enzymes to chilling stress in tobacco seedlings. Agr. Sci. China. 9, 1594–1601.

    CAS  Article  Google Scholar 

  38. 38.

    Yalçin, H. (2010) Effect of ripening period on composition of pepino (Solanum muricatum) fruit grown in Turkey. Afr. J. Biotechnol. 9, 3901–3903.

    Google Scholar 

  39. 39.

    Zhang, W., Jiang, B., Li, W., Song, H., Yu, Y., Chen, J. (2009) Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Sci. Hortic. 122, 200–208.

    CAS  Article  Google Scholar 

  40. 40.

    Zhu, J. J., Li, Y. R., Liao, J. X. (2013) Involvement of anthocyanins in the resistance to chillinginduced oxidative stress in Saccharum officinarum L. leaves. Plant Physiol. Bioch. 73, 427–433.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to A. Kaya.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sivaci, A., Kaya, A. & Duman, S. Effects of Ascorbic Acid on Some Physiological Changes of Pepino (Solanum Muricatum Ait.) under Chilling Stress. BIOLOGIA FUTURA 65, 305–318 (2014). https://doi.org/10.1556/ABiol.65.2014.3.7

Download citation

Keywords

  • Pepino (Solanum muricatum Ait.)
  • ascorbic acid
  • chilling stress
  • physiological parameters