PtLGBP, a Pattern Recognition Receptor in Portunus Trituberculatus Involved in the Immune Response Against Different Challenges
Lipopolysaccharide and b-1,3-glucan binding protein (LGBP) is a pattern recognition receptor that can recognize and bind LPS and b-1,3-glucan. LGBP has crucial roles in innate immune defense against Gram-negative bacteria and fungi. In this study, LGBP functions in Portunus trituberculatus innate immunity were analyzed. First, the mRNA expression of PtLGBP in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (PAMPs) stimulations were examined using real-time PCR. Results show that the overall trend of relative expressions of the LGBP gene in three tissues is consistent, showing up–down trend. In each group, the highest expression of the LGBP gene was at 3 and 12 h post-injection. The LGBP gene is also expressed significantly higher in the hemocytes and hepatopancreas than in the muscle. The highest level of LGBP was in the lipopolysaccharides (LPS) and glucan-injected group, whereas the lowest level was in the PGN-injected group. Furthermore, bacterial agglutination assay with polyclonal antibody specifically for PtLGBP proved that the recombinant PtLGBP (designated as rPtLGBP) could exhibit obvious agglutination activity toward Gramnegative bacteria Escherichia coli, Vibrio parahaemolyticus, and V. alginolyticus; Gram-positive bacteria Bacillus subtilis; and fungi Saccharomyces cerevisiae. LGBP in Portunus trituberculatus possibly served as a multi-functional PRR. In addition, LGBP is not only involved in the immune response against Gramnegative and fungi, as manifested in other invertebrates, but also has a significant role in anti-Grampositive bacteria infection.
KeywordsPortunus trituberculatus LGBP innate immunity pattern recognition receptor
Unable to display preview. Download preview PDF.
- 9.Kim, Y. S., Ryu, J. H., Han, S. J., Choi, K. H., Nam, K. B., Jang, I. H. (2000) Gram-negative bacteriabinding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells. J. Biol. Chem. 275, 32721–32727.CrossRefGoogle Scholar
- 21.Wang, G. L., Shan, J., Chen, Y. E., Li, Z. (2006) Study on pathogens and pathogenesis of emulsification disease of Portunus trituberculatus. Adv. Marine Sci. 24, 527–531.Google Scholar
- 23.Yeh, M. S., Chang, C. C., Cheng, W. (2009) Molecular cloning and characterization of lipopolysaccharide- and beta-1,3-glucan-binding protein from the giant freshwater prawn Macrobrachium rosenbergii and its transcription in relation to foreign material injection and the molt stage. Fish Shellfish Immunol. 27, 701–706.CrossRefGoogle Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.