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Acta Biologica Hungarica

, Volume 65, Issue 3, pp 355–367 | Cite as

Synthetic Seed Production and Physio-Biochemical Studies in Cassia Angustifolia Vahl. — a Medicinal Plant

  • N. A. W. Bukhari
  • Iram SiddiqueEmail author
  • K. Perveen
  • I. Siddiqui
  • M. S. Alwahibi
Article

Abstract

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 ∙ 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1–6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (PN), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.

Keywords

Antioxidant enzymes encapsulation rooting synthetic seeds Thidiazuron 

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • N. A. W. Bukhari
    • 1
  • Iram Siddique
    • 1
    Email author
  • K. Perveen
    • 1
  • I. Siddiqui
    • 1
  • M. S. Alwahibi
    • 1
  1. 1.Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical CollegesKing Saud UniversityRiyadhSaudi Arabia

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