Synthetic Seed Production and Physio-Biochemical Studies in Cassia Angustifolia Vahl. — a Medicinal Plant
Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 ∙ 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1–6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (PN), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.
KeywordsAntioxidant enzymes encapsulation rooting synthetic seeds Thidiazuron
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- 2.Agrawal, V., Sardar, P. R. (2003) In vitro organogenesis and histomorphological investigations in Senna (Cassia angustifolia) a medicinally valuable shrub. Physiol. Mol. Biol. Plants. 91, 131–140.Google Scholar
- 7.Anonymous (1992) The wealth of India: A dictionary of Indian raw materials and industrial products. Vol 3, CSIR, New Delhi, pp. 354–363.Google Scholar
- 14.Faisal, M., Ahmad, N., Anis, M. (2006) In vitro plant regeneration from alginate encapsulated microcuttings of Rauvolfia tetraphylla L. Amer. Eurass. J. Agric. Environ. Sci. 1, 1–6.Google Scholar
- 19.Franz, G. (1993) The senna drug and its chemistry. Pharmocology 47, 2–6.Google Scholar
- 23.Mckinney, G. (1941) Absorption of light by chlorophyll solution. J. Biol. Chem. 140, 315–322.Google Scholar
- 27.Pence, V. C. (1999) The application of biotechnology for the conservation of endangered plants. In: Benson, E. E. (ed.) Plant conservation biotechnology, Vol. 15. Taylor and Francis, London, pp. 227–241.Google Scholar
- 28.Pospisilova, J., Haisel, D., Synkova, H., Catsky, J., Wilhelmova, N., Plzakova, S., Prochazkova, D., Sramek, F. (2000) Photosynthetic pigments and gas exchange during ex vitro acclimation of tobacco plants as affected by CO2 supply and abscisic acid. Plant Cell Tiss. Org. Cult. 61, 125–133.CrossRefGoogle Scholar
- 32.Siddique, I., Anis, M. (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J. Biotechnol. 5, 303–308.Google Scholar
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