Multiplication and Growth of Hybrid Poplar (Populus Alba × P. Tremula) Shoots on a Hormone-Free Medium
- 1 Downloads
The present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.
KeywordsCulture tube explant micropropagation restricted ventilation white poplar
Unable to display preview. Download preview PDF.
- 1.Benschop, J. J., Bou, J., Peeters, A. J. M., Wagemaker, N., Gühl, K., Ward, D., Hedden, P., Moritz, T., Voesenek, L. A. C. J. (2006) Long-term submergence-induced elongation in Rumex palustris requires abscisic acid-dependent biosynthesis of gibberellin1. Plant Physiol. 141, 1644–1652.CrossRefGoogle Scholar
- 5.Castiglione, S., Todeschini, V., Franchin, C., Torrigiani, P., Gastaldi, D., Cicatelli, A., Rinaudo, C., Berta, G., Biondi, S., Lingua, G. (2009) Clonal differences in survival capacity, copper and zinc accumulation, and correlation with leaf polyamine levels in poplar: a large-scale field trial on heavily polluted soil. Environ. Pollut. 157, 2108–2117.CrossRefGoogle Scholar
- 13.Jackson, M. B. (2003) Aeration stress in plant tissue cultures. Bulg. J. Plant Physiol. 29, 96–109.Google Scholar
- 15.Khattab, S. (2011) Effect of different media and growth regulators on the in vitro shoot proliferation of aspen, hybrid aspen and white poplar male tree and molecular analysis of variants in micropropagated plants. Life Sci. J. 8, 177–184.Google Scholar
- 16.Lai, C. C., Yeh, S. D., Yang, J. S. (2000) Enhancement of papaya axillary shoot proliferation in vitro by controlling the available ethylene. Bot. Bull. Acad. Sinica 41, 203–212.Google Scholar
- 19.McCown, B. H., Lloyd, G. (1981) Woody plant medium (WPM)–a mineral nutrient formulation for microculture of woody plant species. HortScience 16, 453.Google Scholar
- 24.Pintarić, B. (2008) Micropropagation of white poplar (Populus alba L.). Šumarski List 132, 343–354. (In Croatian)Google Scholar
- 27.Rédei, K., Keserű, Z., Gábor, S. (2010) Early evaluation of promising white poplar (Populus alba L.) clones in Hungary. Acta Silv. Lign. Hung. 6, 9–16.Google Scholar
- 28.Rijnders, J. G. H. M., Yang, Y. Y., Kamiya, Y., Takahashi, N., Barendse, G. W. M., Blom, C. W. P. M., Voesenek, L. A. C. J. (1997) Ethylene enhances gibberellin levels and petiole sensitivity in floodingtolerant Rumex palustris but not in flooding intolerant R. acetosa. Planta 203, 20–25.CrossRefGoogle Scholar
- 32.Tsvetkov, I., Jouve, L., Hoffmann, L., Hausman, J. F. (2007) The medium composition differentially affects regrowth characteristics in in vitro-derived encapsulated shoot tips of Populus euphratica Oliv. Propag. Ornam. Plants 7, 180–183.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.