Acta Biologica Hungarica

, Volume 65, Issue 2, pp 205–217 | Cite as

Routine Sample Preparation and HPLC Analysis for Ascorbic Acid (Vitamin C) Determination in Wheat Plants and Arabidopsis Leaf Tissues

  • Gabriella SzalaiEmail author
  • T. Janda
  • Magda Pál


Plants have developed various mechanisms to protect themselves against oxidative stress. One of the most important non-enzymatic antioxidants is ascorbic acid. There is thus a need for a rapid, sensitive method for the analysis of the reduced and oxidised forms of ascorbic acid in crop plants. In this paper a simple, economic, selective, precise and stable HPLC method is presented for the detection of ascorbate in plant tissue. The sensitivity, the short retention time and the simple isocratic elution mean that the method is suitable for the routine quantification of ascorbate in a high daily sample number. The method has been found to be better than previously reported methods, because of the use of an economical, readily available mobile phase, UV detection and the lack of complicated extraction procedures. The method has been tested on Arabidopsis plants with different ascorbate levels and on wheat plants during Cd stress.


Ascorbic acid HPLC analysis plant tissue stress UV detection 



ascorbic acid, reduced form


dehydroascorbic acid, oxidised form


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© Akadémiai Kiadó Zrt. 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Plant Physiology, Agricultural Institute, Centre for Agricultural ResearchHungarian Academy of SciencesMartonvásárHungary

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