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Growth Regulator Requirement for In Vitro Embryogenic Cultures of Snowdrop (Galanthus Nivalis L.) Suitable for Germplasm Preservation

Acta Biologica Hungarica volume 65, pages 165–177 (2014)Cite this article

Abstract

In this study, we report on the production of bulb scale-derived tissue cultures capable of efficient shoot and plant regeneration in three genotypes of snowdrop (Galanthus nivalis L., Amaryllidaceae), a protected ornamental plant. For culture line A, high auxin and low cytokinin concentration is required for callus production and plant regeneration. The type of auxin is of key importance: α-naphthaleneacetic acid (NA) in combination with indole-3-acetic acid (IA) at concentrations of 2 mg L–1 or 2–10 mg L–1 NA with 1 mg L–1 N6-benzyladenine (BA), a cytokinin on full-strength media are required for regeneration. Cultures showing regeneration were embryogenic. When lines B and C were induced and maintained with 2 mg L–1 NA and 1 mg L–1 BA, they produced mature bulblets with shoots, without roots. Line A produced immature bulblets with shoots under the above culture condition. Amplified Fragment Length Polymorphism (AFLP) analysis showed that (i) genetic differences between line A and its bulb explants were not significant, therefore these tissue cultures are suitable for germplasm preservation, and (ii) different morphogenetic responses of lines A, B and C originated from genetic differences. Culture line A is suitable for field-growing, cultivation and germplasm preservation of G. nivalis and for the production of Amaryllidaceae alkaloids.

Abbreviations

AA:

ascorbic acid

AFLP:

Amplified Fragment Length Polymorphism

BA:

N6-benzyladenine

CH:

casein hydrolysate

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS medium:

Murashige-Skoog medium

NAA:

α-naphthaleneacetic acid

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Author information

Affiliations

  1. Department of Botany, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032, Debrecen, Hungary

    Anna Resetár, Zita Demeter, Ágnes Mosolygó, S. Gonda, G. Surányi & C. Máthé

  2. Department of Pharmacognosy, Semmelweis University, Üllői út 26, H-1085, Budapest, Hungary

    Emese Ficsor, Andrea Balázs & Éva Szőke

  3. Botanical Garden, University of Debrecen, Egyetem tér 1, H-4032, Debrecen, Hungary

    L. Pap

Authors
  1. Anna Resetár
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  2. Zita Demeter
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  3. Emese Ficsor
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  4. Andrea Balázs
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  5. Ágnes Mosolygó
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  6. Éva Szőke
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  7. S. Gonda
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  8. L. Pap
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  9. G. Surányi
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  10. C. Máthé
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Correspondence to C. Máthé.

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Resetár, A., Demeter, Z., Ficsor, E. et al. Growth Regulator Requirement for In Vitro Embryogenic Cultures of Snowdrop (Galanthus Nivalis L.) Suitable for Germplasm Preservation. BIOLOGIA FUTURA 65, 165–177 (2014). https://doi.org/10.1556/ABiol.65.2014.2.5

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Keywords

  • Galanthus nivalis
  • bulb callus
  • somatic embryogenesis
  • plant regeneration
  • germplasm preservation