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Acta Biologica Hungarica

, Volume 65, Issue 2, pp 156–164 | Cite as

A Preliminary Report on the Genetic Variation in Pointed Gourd (Trichosanthes Dioica Roxb.) as Assessed by Random Amplified Polymorphic DNA

  • S. Adhikari
  • A. Biswas
  • T. K. Bandyopadhyay
  • P. D. GhoshEmail author
Article

Abstract

Pointed gourd (Trichosanthes dioica Roxb.) is an economically important cucurbit and is extensively propagated through vegetative means, viz vine and root cuttings. As the accessions are poorly characterized it is important at the beginning of a breeding programme to discriminate among available genotypes to establish the level of genetic diversity. The genetic diversity of 10 pointed gourd races, referred to as accessions was evaluated. DNA profiling was generated using 10 sequence independent RAPD markers. A total of 58 scorable loci were observed out of which 18 (31.03%) loci were considered polymorphic. Genetic diversity parameters [average and effective number of alleles, Shannon’s index, percent polymorphism, Nei’s gene diversity, polymorphic information content (PIC)] for RAPD along with UPGMA clustering based on Jaccard’s coefficient were estimated. The UPGMA dendogram constructed based on RAPD analysis in 10 pointed gourd accessions were found to be grouped in a single cluster and may represent members of one heterotic group. RAPD analysis showed promise as an effective tool in estimating genetic polymorphism in different accessions of pointed gourd.

Keywords

DNA marker genetic diversity pointed gourd RAPD Trichosanthes dioica 

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© Akadémiai Kiadó Zrt. 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. Adhikari
    • 1
  • A. Biswas
    • 1
  • T. K. Bandyopadhyay
    • 2
  • P. D. Ghosh
    • 1
    Email author
  1. 1.Department of BotanyUniversity of KalyaniKalyaniIndia
  2. 2.Department of Molecular Biology and BiotechnologyUniversity of KalyaniKalyaniIndia

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