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Acta Biologica Hungarica

, Volume 65, Issue 2, pp 144–155 | Cite as

Polyphenol Composition and Antioxidant Capacity from Different Extracts of Aster Scaber

  • M. Thiruvengadam
  • N. Praveen
  • B. R. Yu
  • S. H. Kim
  • I. M. ChungEmail author
Article

Abstract

Phenolic contents and antioxidant capacities from different solvent extracts (petroleum ether, ethyl acetate, methanol, butanol and water) of Aster scaber leaf were investigated. Antioxidant activity was evaluated by three different methods, namely DPPH radical scavenging activity, reducing power assay and phosphomolybdenum activity. A total of twenty-three polyphenolic compounds were identified and quantified from A. scaber leaf extracts, including hydroxybenzoic acids, hydroxycinnamic acids, flavonols and other groups of phenolic compounds. Ultra high performance liquid chromatography (UHPLC) analysis of the leaf extract revealed that myricetin (4850.45 μg/g) was the most dominant flavonols, compared to quercetin and kaempferol. Caffeic acid was the dominant phenolic compound in A. scaber leaf extracts, it constituted about 104.20 μg/g, followed by gentisic acid (84.50 μg/g), gallic acid (61.05 μg/g) and homogentisic acid (55.65 μg/g). The total phenolic and flavonoid content was the highest in ethyl acetate extract (322.43 and 6.51 mg/g). The decreasing order of antioxidant activity among the A. scaber leaf extracts assayed through all the three methods was found to be ethyl acetate > butanol > methanol > petroleum ether > water extract.

Keywords

Leafy vegetables Aster scaber antioxidant activity phenolic compounds flavonoids 

Abbreviations

BHT

butylated hydroxytoluene

DPPH

1,1-diphenyl-2-picrylhydrazyl

GAE

gallic acid equivalents

QE

quercetin equivalents

UHPLC

ultra high performance liquid chromatography

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© Akadémiai Kiadó Zrt. 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. Thiruvengadam
    • 1
  • N. Praveen
    • 1
  • B. R. Yu
    • 1
  • S. H. Kim
    • 1
  • I. M. Chung
    • 1
    Email author
  1. 1.Department of Applied Bioscience, College of Life and Environmental ScienceKonkuk UniversitySeoulSouth Korea

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