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Acta Biologica Hungarica

, Volume 65, Issue 2, pp 132–143 | Cite as

PCR-based identification of Adriatic specimen of three scorpionfish species (Scorpaenidae, Teleostei)

  • J. M. Saju
  • Sz. Németh
  • Réka Szűcs
  • Rashmi Sukumaran
  • Z. Lim
  • L. Wong
  • L. OrbánEmail author
  • M. BercsényiEmail author
Article

Abstract

The identification of three scorpionfish species, the black scorpionfish (Scorpaena porcus Linnaeus, 1758), the large-scaled scorpionfish (S. scrofa Linnaeus, 1758) and the small red scorpionfish (S. notata Rafinesque, 1810) is possible in adults by morphometry, but often problematic in juveniles due to their similar phenotypes. To develop a molecular species identification tool, first, we have analyzed the genetic similarity of the three species by a PCR-based ‘blind method’ that amplified bands from various locations of the genome. We found high levels of nucleotide similarity between S. porcus and S. scrofa, whereas S. notata showed a higher level of divergence from the other two species. Then, we have searched these patterns for differences between the genomes of Adriatic specimen of these three species and identified several species-specific products in two of them. For the third one a species-specific primer pair amplifying from the 16S ribosomal DNA was designed. One marker for each species was cloned, sequenced and converted into Sequence Characterized Amplified Region (SCAR) markers amplified by specific primer pairs. The SCAR markers amplified robust bands of limited variability from the target species, while no or only occasional weak products were obtained from the other two, proving that they can be used for molecular identification of these three species. These markers can help the conservation and future analysis of these three species as well as their possible selection programs for aquaculture purposes.

Keywords

Scorpaena molecular species ID 16S rDNA fluoMEP SCAR molecular aquaculture 

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Copyright information

© Akadémiai Kiadó Zrt. 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • J. M. Saju
    • 1
  • Sz. Németh
    • 2
  • Réka Szűcs
    • 2
  • Rashmi Sukumaran
    • 1
    • 3
  • Z. Lim
    • 1
    • 4
  • L. Wong
    • 3
  • L. Orbán
    • 1
    • 2
    • 4
    Email author
  • M. Bercsényi
    • 2
    Email author
  1. 1.Reproductive Genomics Group, Strategic Research Program, Temasek Life Sciences Laboratory, 1 Research LinkNational University of SingaporeSingapore
  2. 2.Department of Animal Sciences and Breeding, Georgikon FacultyUniversity of PannoniaKeszthelyHungary
  3. 3.School of Computing, National University of SingaporeSingapore
  4. 4.Department of Biological SciencesNational University of SingaporeSingapore

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