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Acta Biologica Hungarica

, Volume 65, Issue 1, pp 47–60 | Cite as

Waterlogging and Nitric Oxide Induce Gene Expression and Increase Antioxidant Enzyme Activity in Wheat (Triticum Aestivum L.)

  • S. Özçubukçu
  • N. ErgünEmail author
  • E. İlhan
Article

Abstract

The effects of waterlogging (WL) and WL plus nitric oxide (WL+NO) were investigated in seedlings of one wheat cultivars (Triticum aestivum cv. Dogankent) and one wheat line (Triticum aestivum cv. Ducula-4). Under WL conditions, catalase activity was greater in Ducula-4 than in Dogankent. Glutathione reductase activity increased in Ducula-4 seedlings under WL+NO conditions, especially at 48 and 72 hours of treatment. Myb2 expression increased during the early hours of treatment in both wheat varieties exposed to WL, with 40-fold higher levels in Ducula-4, gradually decreasing to control levels. Under WL+NO treatment, Myb2 expression increased 44-fold at 12 hours and high levels of expression were still observed at 72 hours. When Ducula-4 seedlings were subjected to WL+NO treatment, PDPK expression increased approximately 15-fold at 3 hours and decreased to control levels at 72 hours. Under the same conditions, SST1 expression increased 3-fold at 3 and 12 hours and reached control levels during the subsequent hours. Among the genes studied, the highest level of expression was observed for Myb2. Moreover, gene expression was altered most by waterlogging in Ducula-4 seedlings.

Keywords

Wheat waterlogging nitric oxide quantitative RT-PCR Myb2 SST PDPK 

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References

  1. 1.
    Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y., Sakuratani, T. (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean/subjected to waterlogging, Plant Sci. 163, 117–123.CrossRefGoogle Scholar
  2. 2.
    Allen, R. (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol. 107, 1049–1054.CrossRefGoogle Scholar
  3. 3.
    Baxter-Burrell, A., Chang, R., Springer, P., Bailey-Serres, J. (2003) Gene and enhancer trap transposable elements reveal oxygen deprivation regulated genes and their complex patterns of expression in Arabidopsis. Annals Bot. 91, 129–141.CrossRefGoogle Scholar
  4. 4.
    Beligni, M. V., Lamattina, L. (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210, 215–221.CrossRefGoogle Scholar
  5. 5.
    Bowler, C., Montagu, M. V., Inze, D. (1992) Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 83–116.CrossRefGoogle Scholar
  6. 6.
    Chaitanya, K.V., Sundar, D., Masilamani, S., Ramachandra Reddy, A. (2002) Variation in heat stressinduced antioxidant enzyme activities among three mulberry cultivars. Plant Growth Regul. 36, 175–180.Google Scholar
  7. 7.
    Clarke, A., Desikan, R., Hurst, R. D., Hancock, J. T., Neill, S. J. (2000) NO way back: nitric oxide and programmed cell death in Arabidopsis thaliana suspension cultures. The Plant Journal 24, 1–13.CrossRefGoogle Scholar
  8. 8.
    Correa-Aragunde, N., Graziano, M., Chevalier, C., Lamattina, L. (2006) Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato. J. Exp. Bot. 57, 581–588.CrossRefGoogle Scholar
  9. 9.
    Çakmak, I., Marschner, H. (1992) Magnesium deficiency and high-light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol. 98, 1222–1227.CrossRefGoogle Scholar
  10. 10.
    Çakmak, I. (1994) Activity of ascorbate-dependet H2O2 scavenging enzymes and leaf cholorosis are enhanced in magnesium and potassium deficient leaves, but not in phosphorus deficient leaves. J. Exp. Bot. 45, 1259–1266.CrossRefGoogle Scholar
  11. 11.
    Dat, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D., Van Breusegem, F. (2000) Dual action of the active oxygen species during plant stress responses. Cell. Mol. Life. Sci. 57, 779–795.CrossRefGoogle Scholar
  12. 12.
    Delledonne, M., Zeier, J., Marocco, A., Lamb, C. (2001) Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. PNAS 98, 13454–13459.CrossRefGoogle Scholar
  13. 13.
    D olferus, R., Klok, E. J., Delessert, C., Wilson, S., Ismond, K. P., Good, A. G., Peacock, W. J., Dennis, E. S. (2003) Enhancing the anaerobic response. Ann. Bot. 91, 111–117.CrossRefGoogle Scholar
  14. 14.
    Dordas, C., Rivoal, J., Hill, R. D. (2003) Plant haemoglobins, nitric oxide and hypoxic stress. Ann. Bot. 91, 173–178.CrossRefGoogle Scholar
  15. 15.
    Durner, J., Wendehenne, D., Klessig, D. F. (1998) Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclicADPribose. PNAS 95, 10328–10333.CrossRefGoogle Scholar
  16. 16.
    Food and Agriculture Organization of the United Nations (FAO), (2005) FAO Statistical Databases, https://doi.org/www.apps.fao.orgGoogle Scholar
  17. 17.
    Gerendás, J., Ratcliffe, R. G. (2002) Root pH control. In: Y. Waisel, A. Eshel, U. Kafkafi (eds) Plant Roots the Hidden Half. Marcel Deker Inc., New York, pp. 553–570.CrossRefGoogle Scholar
  18. 18.
    Keles, Y., Ünyayar, S. (2004) Responses of antioxidant defence system of Helianthus annuus to abscisic acid treatment under drought and waterlogging, Acta Physiol. Plant. 26, 149–156.CrossRefGoogle Scholar
  19. 19.
    Lee, T. G., Jang, C. S., Kim, J. Y., Dong Sub Kim, D. S., Park, J. H., Kim, D. Y., Seo, Y. W. (2007) A Myb transcription factor (TaMyb1) from wheat roots is expressed during hypoxia:roles in response to the oxygen concentration in root environment and abiotic stresses. Physiol. Plant. 129, 375–385.CrossRefGoogle Scholar
  20. 20.
    Li, C., Jianga, D., Wollenweberb, B., Li, Y., Daia, T., Caoa, W. (2011) Waterlogging pretreatment during vegetative growth improves tolerance to waterlogging after anthesis in wheat. Plant Sci. 180, 672–678.CrossRefGoogle Scholar
  21. 21.
    Neill, S. J., Desikan, R., Hancock, J. T. (2002) Nitric oxide signalling in plants. New Phytol. 159, 11–35.Google Scholar
  22. 22.
    Polle, A. (2001) Dissecting the superoxide dismutase-ascorbate glutathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiol. 126, 445–462.CrossRefGoogle Scholar
  23. 23.
    Polverari, A., Molesini, B., Pezzotti, M., Buonaurio, R., Marte, M., Delledonne, M. (2003) Nitric oxide-mediated transcriptional changes in Arabidopsis thaliana. Mol. Plant-Microbe Interact. 16, 1094–1105.CrossRefGoogle Scholar
  24. 24.
    Radi, R., Beckam, J. S., Bash, K. M., Freeman, R. A. (1991) Peroxynitrite induced membrane lipid peroxidation: cytotoxic potential of superoxide and nitric oxide. Arch. Biochem. Biophys. 228, 481–487.CrossRefGoogle Scholar
  25. 25.
    Rahaie, M., Xue, G. P., Naghavi, M. R., Alizadeh, H., Schenk, P. M. (2010) A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Rep. 29, 835–844.CrossRefGoogle Scholar
  26. 26.
    Sairam, R. K., Dharmar, K., Chinnusamy, V., Meena, R. C. (2009) Waterlogging-induced increase in sugar mobilization, fermentation, and related gene expression in the roots of mung bean (Vigna radiata). J. Plant Physiol. 166, 602–616.CrossRefGoogle Scholar
  27. 27.
    Sayre, K. D., van Ginkel, M., Rajaram, S., Ortiz-Monasterio, I. (1994) Tolerance to water-logging losses in spring bread wheat: effect of time of onset on expression. In: Annual Wheat Newsletter No. 40. Colorado State University, pp. 165–171.Google Scholar
  28. 28.
    Van Der Mescht, A., De Ronde, J. A., Van Der Merwe, T., Rossouw, F. T. (1998). Changes in free proline concentrations and polyamine levels in potato leaves during drought stress. S. African J. Sci. 94, 347–350.Google Scholar

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© Akadémiai Kiadó, Budapest 2014

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Biology Department, Science and Art FacultyMustafa Kemal UniversityAntakya, HatayTurkey
  2. 2.Altinözü Vocational SchoolMustafa Kemal UniversityAltinözü, HatayTurkey

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