In Vitro Antifungal Activity of Medicinal Plant Extracts against Fusarium Oxysporum f. sp. Lycopersici Race 3 the Causal Agent of Tomato Wilt
Medicinal plant extracts of five plants; Adhatoda vasica, Eucalyptus globulus, Lantana camara, Nerium oleander and Ocimum basilicum collected from Cairo, Egypt were evaluated against Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. The results revealed that cold distilled water extracts of O. basilicum and E. globulus were the most effective ones for inhibiting the growth of F. oxysporum f. sp. lycopersici. Butanolic and ethanolic extracts of the tested plants inhibited the pathogen growth to a higher extent than water extracts. Butanolic extract of O. basilicum completely inhibited the growth of F. oxysporum f. sp. lycopersici at concentrations 1.5 and 2.0% (v/v). Butanolic extracts (2.0%) of tested plants had a strong inhibitory effect on hydrolytic enzymes; ß-glucosidase, pectin lyase and protease of F. oxysporum f. sp. lycopersici. This study has confirmed that the application of plant extracts, especially from O. basilicum for controlling F. oxysporum f. sp. lycopersici is environmentally safe, cost effective and does not disturb ecological balance. Investigations are in progress to test the efficacy of O. basilicum extract under in vivo conditions.
KeywordsOcimum basilicum Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions antifungal activity hydrolytic enzymes
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- 7.Asthana, A., Chandra, H., Dikshit, A., Dixit, S. N. (1982) Volatile fungitoxicants from leaves of some higher plants against Helminthosporium oryzae. Z. Pflanzenkr. Pflanzenschutz. 89, 475–479.Google Scholar
- 8.Asthana, A., Dixit, K., Tripathi, N. N., Dixit, S. N. (1989) Efficacy of Ocimum oil against fungi attacking chilli seed during storage. Trop. Sci. 29, 15–20.Google Scholar
- 9.Bansod, S., Rai, M. (2008) Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and A. niger. World J. Med. Sci. 3, 81–88.Google Scholar
- 10.Baraka, M. A., Omar, S. A., El-Barougy, E., Zian, A. H. (2006) Controlling seedling damping-off, root rot and wilt diseases of lupine (Lupinus albus L.). Agric. Res. J. Suez Canal Univ. 6, 57–68.Google Scholar
- 12.Dissanayake, M. L. M. C., Kumari, W. K. M. T. (2012) Efficacy of various plant extracts to control Fusarium wilt of variety Marginata Fusarium Polyscias Balfouriana. Asian J. Exp. Biol. Sci. 3, 129–135.Google Scholar
- 14.Fawzi, E. M., Khalil, A. A., Afifi, A. F. (2009) Antifungal effect of some plant extracts on Alternaria alternata and Fusarium oxysporum. Afr. J. Biotechnol. 8, 2590–2597.Google Scholar
- 16.Ibrahim, G. H. (1997) Studies on some wheat disease with reference to crown root-rot disease caused by Fusarium culmorum. PhD Thesis, Faculty of Agriculture, Alexandria University, Egypt.Google Scholar
- 17.Kagale, S., Marimuthu, T., Thayumanavan, B., Nandakumar, R., Samiyappan, R. (2004) Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiol. Mol. Plant Pathol. 65, 91–100.CrossRefGoogle Scholar
- 18.Kiran, K., Linguraju, S., Adiver, S. (2006) Effect of plant extract on Sclerotium rolfsii, the incitant of stem rot of ground nut. J. Mycol. Plant Pathol. 36, 77–79.Google Scholar
- 19.Kumar, A., Roy, S. K., Saxena, D. C., Saxena, A. R. (1995) In vitro control of Escherichia coli by herbal treatment. Neo Botanica 3, 1–2.Google Scholar
- 21.List, P. H., Hörhammer, L. (1977) Hagers Handbuch der Pharmazeutischen Praxis. 4. Aufl., Band VI A, Springer-Verlag, Berlin-Heidelberg, Germany.Google Scholar
- 25.Mohamed, N. H., El-Hadidy, A. M. (2008) Studies of biologically active constituents of Verbascum eremobium Murb. and its inducing resistance against some diseases of cucumber. Egypt. J. Phytopathol. 36, 133–150.Google Scholar
- 26.Nahak, G., Mishra, R. C., Sahu, R. K. (2011) Taxonomic distribution, medicinal properties and drug development potentiality of Ocimum (Tulsi). Drug Inven. Today 3, 95–113.Google Scholar
- 27.Nduagu, C., Ekefan, E. J., Nwankiti, A. O. (2008) Effect of some crude plant extracts on growth of Colletotrichum capsici (Synd) Butler & Bisby, causal agent of pepper anthracnose. J. Appl. Biosci. 6, 184–190.Google Scholar
- 30.Saxena, A. R., Sahni, R. K., Yadav, H. L., Upadhyay, S. K., Saxena, M. (2005) Antifungal activity of some higher plants against Fusarium oxysporum f. sp. pisi. J. Liv. World 12, 32–39.Google Scholar
- 31.Serra, J. L., Alkorta, I., Llama, M. J., Alaña, A. (1992) Aplication industrial de los enzimas pecticos. Produccion, purificacion, inmovilizacion yalgunas propiedades de la pectina liasa de Penicillium italicum. Alimentacion, Equipos y Technologia 8, 127–133.Google Scholar
- 32.Shrivastava, A., Rizvi, G., Paijwar, M. S. (2011) Antifungal activity of some wild medicinal plants against growth of Fusarium oxysporum f. sp. Zingiber fficinales Rosc. J. Pharm. Herb. Form. 1, 24–27.Google Scholar
- 33.Somda, I., Leth, V., Seeme, P. (2007) Evaluation of lemongrass, eucalyptus and neem aqueous extracts for controlling seed-borne fungi of sorghum grown in Burkina Faso. World J. Agric. Sci. 3, 218–223.Google Scholar
- 34.Viorica, H. (1987) Polyphenols of Ocimum basilicum L. Clujul Med. 60, 340–344.Google Scholar
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