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Acta Biologica Hungarica

, Volume 64, Issue 3, pp 279–288 | Cite as

Comparative Study Concerning Mistletoe Viscotoxins Antitumor Activity

  • Roxana Liana StanEmail author
  • Adriana Corina Hangan
  • Lucia Dican
  • B. Sevastre
  • Daniela Hanganu
  • C. Catoi
  • Orsolya Sarpataki
  • Corina Maria Ionescu
Article

Abstract

Viscum album L. (Santalaceae) (VA) - a parasitic plant that grows on various trees - has proved a significant anticancer effect in both experimental studies and clinical trials. The present study assesses the influence of oxidative stress in mistletoe induced cytotoxicity in tumor cells, in relation to classic cytostatic therapy. VA ethanolic extract was administered alone and combined with doxorubicin (chloride) in Swiss female mice previously intraperitoneally (i.p.) inoculated with Ehrlich tumor cells (1×106/animal) that consequently developed Ehrlich ascites carcinoma (EAC). The administered doses were of 50 mg/kg on the 1st, 3rd and 6th day for the VA extract, respectively of 2.5 mg/kg on the 1st and 6th day for doxorubicin, after tumor cell implantation. Fourteen days later all mice were euthanized, ascites of the EAC were collected in order to analyze the tumor proliferation parameters, as well as blood samples, in order to evaluate the antioxidant status in plasma. Tumor development was associated with increased activity of plasma enzymes; classic doxorubicin therapy not only prevents the accumulation of ascitic fluid, but also significantly reduces the activity of plasma antioxidant enzymes. Furthermore, in association with VA extract, the protective effect is improved. Oxidative changes in Ehrlich tumor cells consisted in decreased catalase activity and amplified xanthine oxidase and peroxidase activities.

Keywords

Mistletoe mice cancer oxidative stress doxorubicin 

Abbreviations

b.w.

body weight

CAT

catalase

EAC

Ehrlich Ascites Carcinoma

i.p.

intra-peritoneal

Px

peroxidase

XOD

xanthine oxidase

VA

Viscum album

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Notes

Acknowledgements

This study was partially supported by POSDRU/89/1.5/S/62371.

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Copyright information

© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Roxana Liana Stan
    • 1
    Email author
  • Adriana Corina Hangan
    • 2
  • Lucia Dican
    • 3
  • B. Sevastre
    • 4
  • Daniela Hanganu
    • 5
  • C. Catoi
    • 4
  • Orsolya Sarpataki
    • 4
  • Corina Maria Ionescu
    • 1
  1. 1.Department of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania
  2. 2.Department of Inorganic Chemistry, Faculty of Pharmacy“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania
  3. 3.Department of Biochemical Medicine, Faculty of Medicine“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania
  4. 4.Faculty of Veterinary MedicineUniversity of Agricultural Science and Veterinary MedicineCluj-NapocaRomania
  5. 5.Department of Pharmacognosy, Faculty of Pharmacy“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania

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